An aqueous extract of tuberaria lignosa inhibits cell growth, alters the cell cycle profile, and induces apoptosis of NCI-H460 tumor cells
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10198/13486 |
Resumo: | Tuberaria lignosa (Sweet) Samp. is found in European regions, and has antioxidant properties due to its composition in ascorbic acid and phenolic compounds. Given its traditional use and antioxidant properties, the tumor cell growth inhibitory potential of aqueous extracts from T. lignosa (prepared by infusion and decoction) was investigated in three human tumor cell lines: MCF-7 (breast adenocarcinoma), NCI-H460 (non-small cell lung cancer), and HCT-15 (human colorectal adenocarcinoma). Both extracts inhibited the growth of these cell lines; the most potent one being the T. lignosa extract obtained by infusion in the NCI-H460 cells (GI50 of approximately 50 μg/mL). Further assays were carried out with this extract in NCI-H460 cells. At 100 μg/mL or 150 μg/mL it caused an increase in the percentage of cells in the G0/G1 phase and a decrease of cells in S phase of the cell cycle. Additionally, these concentrations caused an increase in the percentage of apoptotic cells. In agreement, a decrease in total poly (ADP-ribose) polymerase (PARP) and pro-caspase 3 levels was found. In conclusion, the T. lignosa extract obtained by infusion was more potent in NCI-H460 cells, altering the cell cycle progression and inducing apoptosis. This work highlights the importance of T. lignosa as a source of bioactive compounds with tumor cell growth inhibitory potential. |
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An aqueous extract of tuberaria lignosa inhibits cell growth, alters the cell cycle profile, and induces apoptosis of NCI-H460 tumor cellsTuberaria lignosa (Sweet) Samp. is found in European regions, and has antioxidant properties due to its composition in ascorbic acid and phenolic compounds. Given its traditional use and antioxidant properties, the tumor cell growth inhibitory potential of aqueous extracts from T. lignosa (prepared by infusion and decoction) was investigated in three human tumor cell lines: MCF-7 (breast adenocarcinoma), NCI-H460 (non-small cell lung cancer), and HCT-15 (human colorectal adenocarcinoma). Both extracts inhibited the growth of these cell lines; the most potent one being the T. lignosa extract obtained by infusion in the NCI-H460 cells (GI50 of approximately 50 μg/mL). Further assays were carried out with this extract in NCI-H460 cells. At 100 μg/mL or 150 μg/mL it caused an increase in the percentage of cells in the G0/G1 phase and a decrease of cells in S phase of the cell cycle. Additionally, these concentrations caused an increase in the percentage of apoptotic cells. In agreement, a decrease in total poly (ADP-ribose) polymerase (PARP) and pro-caspase 3 levels was found. In conclusion, the T. lignosa extract obtained by infusion was more potent in NCI-H460 cells, altering the cell cycle progression and inducing apoptosis. This work highlights the importance of T. lignosa as a source of bioactive compounds with tumor cell growth inhibitory potential.This work was financed by FEDER—Fundo Europeu de Desenvolvimento Regional funds through the COMPETE 2020—Operacional Programme for Competitiveness and Internationalisation (POCI), Portugal 2020, and by Portuguese funds through FCT—Fundação para a Ciência e a Tecnologia/ Ministério da Ciência, Tecnologia e Inovação in the framework of the project "Institute for Research and Innovation in Health Sciences" (POCI-01-0145-FEDER-007274). The authors thank the Portuguese Foundation for Science and Technology (FCT) for the PhD grant of V.L-R. (SFRH/BD/87646/2012) and for the post-doc grant of R.T.L. (SFRH/BPD/68787/2010).Biblioteca Digital do IPBPereira, Joana M.Lopes-Rodrigues, VanessaXavier, CristinaLima, M. JoãoLima, Raquel T.Ferreira, Isabel C.F.R.Vasconcelos, M. Helena2016-11-14T12:17:58Z20162016-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/13486engPereira, Joana M.; Lopes-Rodrigues, Vanessa; Xavier, Cristina; Lima, M. João; Lima, Raquel T.; Ferreira, Isabel C.F.R.; Vasconcelos, M. Helena (2016). An aqueous extract of tuberaria lignosa inhibits cell growth, alters the cell cycle profile, and induces apoptosis of NCI-H460 tumor cells. Molecules. ISSN 1420-3049. 21:5, p. 1-81420-304910.3390/molecules21050595info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-01-16T12:15:40ZPortal AgregadorONG |
dc.title.none.fl_str_mv |
An aqueous extract of tuberaria lignosa inhibits cell growth, alters the cell cycle profile, and induces apoptosis of NCI-H460 tumor cells |
title |
An aqueous extract of tuberaria lignosa inhibits cell growth, alters the cell cycle profile, and induces apoptosis of NCI-H460 tumor cells |
spellingShingle |
An aqueous extract of tuberaria lignosa inhibits cell growth, alters the cell cycle profile, and induces apoptosis of NCI-H460 tumor cells Pereira, Joana M. |
title_short |
An aqueous extract of tuberaria lignosa inhibits cell growth, alters the cell cycle profile, and induces apoptosis of NCI-H460 tumor cells |
title_full |
An aqueous extract of tuberaria lignosa inhibits cell growth, alters the cell cycle profile, and induces apoptosis of NCI-H460 tumor cells |
title_fullStr |
An aqueous extract of tuberaria lignosa inhibits cell growth, alters the cell cycle profile, and induces apoptosis of NCI-H460 tumor cells |
title_full_unstemmed |
An aqueous extract of tuberaria lignosa inhibits cell growth, alters the cell cycle profile, and induces apoptosis of NCI-H460 tumor cells |
title_sort |
An aqueous extract of tuberaria lignosa inhibits cell growth, alters the cell cycle profile, and induces apoptosis of NCI-H460 tumor cells |
author |
Pereira, Joana M. |
author_facet |
Pereira, Joana M. Lopes-Rodrigues, Vanessa Xavier, Cristina Lima, M. João Lima, Raquel T. Ferreira, Isabel C.F.R. Vasconcelos, M. Helena |
author_role |
author |
author2 |
Lopes-Rodrigues, Vanessa Xavier, Cristina Lima, M. João Lima, Raquel T. Ferreira, Isabel C.F.R. Vasconcelos, M. Helena |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Biblioteca Digital do IPB |
dc.contributor.author.fl_str_mv |
Pereira, Joana M. Lopes-Rodrigues, Vanessa Xavier, Cristina Lima, M. João Lima, Raquel T. Ferreira, Isabel C.F.R. Vasconcelos, M. Helena |
description |
Tuberaria lignosa (Sweet) Samp. is found in European regions, and has antioxidant properties due to its composition in ascorbic acid and phenolic compounds. Given its traditional use and antioxidant properties, the tumor cell growth inhibitory potential of aqueous extracts from T. lignosa (prepared by infusion and decoction) was investigated in three human tumor cell lines: MCF-7 (breast adenocarcinoma), NCI-H460 (non-small cell lung cancer), and HCT-15 (human colorectal adenocarcinoma). Both extracts inhibited the growth of these cell lines; the most potent one being the T. lignosa extract obtained by infusion in the NCI-H460 cells (GI50 of approximately 50 μg/mL). Further assays were carried out with this extract in NCI-H460 cells. At 100 μg/mL or 150 μg/mL it caused an increase in the percentage of cells in the G0/G1 phase and a decrease of cells in S phase of the cell cycle. Additionally, these concentrations caused an increase in the percentage of apoptotic cells. In agreement, a decrease in total poly (ADP-ribose) polymerase (PARP) and pro-caspase 3 levels was found. In conclusion, the T. lignosa extract obtained by infusion was more potent in NCI-H460 cells, altering the cell cycle progression and inducing apoptosis. This work highlights the importance of T. lignosa as a source of bioactive compounds with tumor cell growth inhibitory potential. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-11-14T12:17:58Z 2016 2016-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10198/13486 |
url |
http://hdl.handle.net/10198/13486 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pereira, Joana M.; Lopes-Rodrigues, Vanessa; Xavier, Cristina; Lima, M. João; Lima, Raquel T.; Ferreira, Isabel C.F.R.; Vasconcelos, M. Helena (2016). An aqueous extract of tuberaria lignosa inhibits cell growth, alters the cell cycle profile, and induces apoptosis of NCI-H460 tumor cells. Molecules. ISSN 1420-3049. 21:5, p. 1-8 1420-3049 10.3390/molecules21050595 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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