Allergy to grass pollen – Mapping of Dactylis glomerata and Phleum pratense allergens for dogs by two-dimensional immunoblotting.
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10174/22097 https://doi.org/DOI: https://doi.org/10.5114/ada.2017.65623. |
Resumo: | Introduction: Much less is known about grass-pollen allergens to dogs, when compared with humans. Genetic based patterns might play an important role in sensitization profiles, conditioning the success of allergen-specific immunotherapy. Aim: Mapping of Dactylis glomerata (D. glomerata) and Phleum pratense (P. pratense) allergens for grass pollensensitized atopic dogs, for better understanding how individual allergograms may influence the response to grasspollen immunotherapy. Material and methods: To identify D. glomerata and P. pratense allergoms for dogs, 15 individuals allergic to grass pollen and sensitized to D. glomerata and P. pratense were selected. D. glomerata and P. pratense proteomes were separated by isoelectric focusing (IEF), one-dimensional (1-D) and two-dimensional (2-D) sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Separated proteins were blotted onto Polyvinylidene difluoride (PVDF) membranes and allergens were identified by patient sera IgE in Western Blotting (WB). Results: In D. glomerata, 17 allergens were identified from IEF and 11 from 1-D SDS-PAGE, while from P. pratense, 18 and 6 allergens were identified, respectively. From 2-D SDS-PAGE 13 spots were identified from D. glomerata and 27 from P. pratense. Conclusions: Several similarities were found between dog and human D. glomerata and P. pratense sensitization profiles but no relationship between clinical signs and a specific pattern of allergen recognition was observed. Similarities were found in each patient pattern of sensitization between D. glomerata and P. pratense, also suggesting cross-reactive phenomena. Further molecular epidemiology approach is needed to understand the role of the sensitization pattern in allergen-specific immunotherapy effectiveness in grass-pollen allergic dogs. |
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Allergy to grass pollen – Mapping of Dactylis glomerata and Phleum pratense allergens for dogs by two-dimensional immunoblotting.allergensatopydoggrass pollen allergensimmunoblottingIntroduction: Much less is known about grass-pollen allergens to dogs, when compared with humans. Genetic based patterns might play an important role in sensitization profiles, conditioning the success of allergen-specific immunotherapy. Aim: Mapping of Dactylis glomerata (D. glomerata) and Phleum pratense (P. pratense) allergens for grass pollensensitized atopic dogs, for better understanding how individual allergograms may influence the response to grasspollen immunotherapy. Material and methods: To identify D. glomerata and P. pratense allergoms for dogs, 15 individuals allergic to grass pollen and sensitized to D. glomerata and P. pratense were selected. D. glomerata and P. pratense proteomes were separated by isoelectric focusing (IEF), one-dimensional (1-D) and two-dimensional (2-D) sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Separated proteins were blotted onto Polyvinylidene difluoride (PVDF) membranes and allergens were identified by patient sera IgE in Western Blotting (WB). Results: In D. glomerata, 17 allergens were identified from IEF and 11 from 1-D SDS-PAGE, while from P. pratense, 18 and 6 allergens were identified, respectively. From 2-D SDS-PAGE 13 spots were identified from D. glomerata and 27 from P. pratense. Conclusions: Several similarities were found between dog and human D. glomerata and P. pratense sensitization profiles but no relationship between clinical signs and a specific pattern of allergen recognition was observed. Similarities were found in each patient pattern of sensitization between D. glomerata and P. pratense, also suggesting cross-reactive phenomena. Further molecular epidemiology approach is needed to understand the role of the sensitization pattern in allergen-specific immunotherapy effectiveness in grass-pollen allergic dogs.Termedia2018-02-08T12:05:25Z2018-02-082017-02-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10174/22097http://hdl.handle.net/10174/22097https://doi.org/DOI: https://doi.org/10.5114/ada.2017.65623.porMartins L, Marques A, Pereira L, Semião-Santos S, Bento O. Adv Dermatol Allergol 2017; XXXIV (1):60–69https://www.termedia.pl/Allergy-to-grass-pollen-mapping-of-Dactylis-glomerata-and-Phleum-pratense-allergens-for-dogs-by-two-dimensional-immunoblotting,7,29334,1,1.htmllmlm@uevora.ptgrilo.deia@gmail.comluisadsp@sapo.ptsaulix@gmail.comofelia@uevora.pt382Martins, LuísMarques, AndreiaPereira, LuísaSemião-Santos, SaulBento, Oféliainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-08-08T04:28:17ZPortal AgregadorONG |
dc.title.none.fl_str_mv |
Allergy to grass pollen – Mapping of Dactylis glomerata and Phleum pratense allergens for dogs by two-dimensional immunoblotting. |
title |
Allergy to grass pollen – Mapping of Dactylis glomerata and Phleum pratense allergens for dogs by two-dimensional immunoblotting. |
spellingShingle |
Allergy to grass pollen – Mapping of Dactylis glomerata and Phleum pratense allergens for dogs by two-dimensional immunoblotting. Martins, Luís allergens atopy dog grass pollen allergens immunoblotting |
title_short |
Allergy to grass pollen – Mapping of Dactylis glomerata and Phleum pratense allergens for dogs by two-dimensional immunoblotting. |
title_full |
Allergy to grass pollen – Mapping of Dactylis glomerata and Phleum pratense allergens for dogs by two-dimensional immunoblotting. |
title_fullStr |
Allergy to grass pollen – Mapping of Dactylis glomerata and Phleum pratense allergens for dogs by two-dimensional immunoblotting. |
title_full_unstemmed |
Allergy to grass pollen – Mapping of Dactylis glomerata and Phleum pratense allergens for dogs by two-dimensional immunoblotting. |
title_sort |
Allergy to grass pollen – Mapping of Dactylis glomerata and Phleum pratense allergens for dogs by two-dimensional immunoblotting. |
author |
Martins, Luís |
author_facet |
Martins, Luís Marques, Andreia Pereira, Luísa Semião-Santos, Saul Bento, Ofélia |
author_role |
author |
author2 |
Marques, Andreia Pereira, Luísa Semião-Santos, Saul Bento, Ofélia |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Martins, Luís Marques, Andreia Pereira, Luísa Semião-Santos, Saul Bento, Ofélia |
dc.subject.por.fl_str_mv |
allergens atopy dog grass pollen allergens immunoblotting |
topic |
allergens atopy dog grass pollen allergens immunoblotting |
description |
Introduction: Much less is known about grass-pollen allergens to dogs, when compared with humans. Genetic based patterns might play an important role in sensitization profiles, conditioning the success of allergen-specific immunotherapy. Aim: Mapping of Dactylis glomerata (D. glomerata) and Phleum pratense (P. pratense) allergens for grass pollensensitized atopic dogs, for better understanding how individual allergograms may influence the response to grasspollen immunotherapy. Material and methods: To identify D. glomerata and P. pratense allergoms for dogs, 15 individuals allergic to grass pollen and sensitized to D. glomerata and P. pratense were selected. D. glomerata and P. pratense proteomes were separated by isoelectric focusing (IEF), one-dimensional (1-D) and two-dimensional (2-D) sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Separated proteins were blotted onto Polyvinylidene difluoride (PVDF) membranes and allergens were identified by patient sera IgE in Western Blotting (WB). Results: In D. glomerata, 17 allergens were identified from IEF and 11 from 1-D SDS-PAGE, while from P. pratense, 18 and 6 allergens were identified, respectively. From 2-D SDS-PAGE 13 spots were identified from D. glomerata and 27 from P. pratense. Conclusions: Several similarities were found between dog and human D. glomerata and P. pratense sensitization profiles but no relationship between clinical signs and a specific pattern of allergen recognition was observed. Similarities were found in each patient pattern of sensitization between D. glomerata and P. pratense, also suggesting cross-reactive phenomena. Further molecular epidemiology approach is needed to understand the role of the sensitization pattern in allergen-specific immunotherapy effectiveness in grass-pollen allergic dogs. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-02-01T00:00:00Z 2018-02-08T12:05:25Z 2018-02-08 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10174/22097 http://hdl.handle.net/10174/22097 https://doi.org/DOI: https://doi.org/10.5114/ada.2017.65623. |
url |
http://hdl.handle.net/10174/22097 https://doi.org/DOI: https://doi.org/10.5114/ada.2017.65623. |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Martins L, Marques A, Pereira L, Semião-Santos S, Bento O. Adv Dermatol Allergol 2017; XXXIV (1):60–69 https://www.termedia.pl/Allergy-to-grass-pollen-mapping-of-Dactylis-glomerata-and-Phleum-pratense-allergens-for-dogs-by-two-dimensional-immunoblotting,7,29334,1,1.html lmlm@uevora.pt grilo.deia@gmail.com luisadsp@sapo.pt saulix@gmail.com ofelia@uevora.pt 382 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Termedia |
publisher.none.fl_str_mv |
Termedia |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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