Metabolic profiling and biological capacity of Pieris brassicae fed with kale (Brassica oleracea L. var. acephala).
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10198/3103 |
Resumo: | Phenolic and organic acid profiles of aqueous extracts from Pieris brassicae material and the host kale (Brassica oleracea L. var. acephala) leaves were determined by HPLC/UV–DAD/MSn-ESI and HPLC–UV, respectively. The identified phenolics included acylated and nonacylated flavonoid glycosides, hydroxycinnamic acyl gentiobiosides, and sulphate phenolics. Kale exhibited the highest content (11 g/kg lyophilized extract), while no phenolics were identified in the butterflies or exuviae. Nine different organic acids were characterized in the materials, with kale showing the highest amount (112 g/kg lyophilized extract). With the exception of the exuviae extract, the rest were screened for bioactivity. Using spectrophotometric microassays, all exhibited antiradical capacity against DPPH and NO in a concentrationdependent way, whereas only kale and excrement extracts were active against superoxide. All displayed activity on intestinal smooth muscle, albeit with distinct relaxation–contraction profiles. Larvae and butterfly extracts were more efficacious for intestinal relaxation than was kale extract, whereas excrement extract evoked only contractions, thus evidencing their different compositions. Collectively, these results show that P. brassicae sequesters and metabolizes kale’s phenolic compounds. Moreover, the extract’s bioactivities suggest that they may constitute an interesting source of bioactive compounds whose complex chemical structures preclude either synthesis or isolation. |
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Metabolic profiling and biological capacity of Pieris brassicae fed with kale (Brassica oleracea L. var. acephala).Pieris brassicae L.Brassica oleracea L. var. acephalaPhenolic compoundsOrganic acidsBiological activityPhenolic and organic acid profiles of aqueous extracts from Pieris brassicae material and the host kale (Brassica oleracea L. var. acephala) leaves were determined by HPLC/UV–DAD/MSn-ESI and HPLC–UV, respectively. The identified phenolics included acylated and nonacylated flavonoid glycosides, hydroxycinnamic acyl gentiobiosides, and sulphate phenolics. Kale exhibited the highest content (11 g/kg lyophilized extract), while no phenolics were identified in the butterflies or exuviae. Nine different organic acids were characterized in the materials, with kale showing the highest amount (112 g/kg lyophilized extract). With the exception of the exuviae extract, the rest were screened for bioactivity. Using spectrophotometric microassays, all exhibited antiradical capacity against DPPH and NO in a concentrationdependent way, whereas only kale and excrement extracts were active against superoxide. All displayed activity on intestinal smooth muscle, albeit with distinct relaxation–contraction profiles. Larvae and butterfly extracts were more efficacious for intestinal relaxation than was kale extract, whereas excrement extract evoked only contractions, thus evidencing their different compositions. Collectively, these results show that P. brassicae sequesters and metabolizes kale’s phenolic compounds. Moreover, the extract’s bioactivities suggest that they may constitute an interesting source of bioactive compounds whose complex chemical structures preclude either synthesis or isolation.FCTElsevierBiblioteca Digital do IPBFerreres, FredericoFernandes, FátimaOliveira, Jorge M.A.Valentão, PatríciaPereira, J.A.Andrade, Paula B.2011-01-13T15:46:42Z20092009-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/3103engFerreres, F.; Fernandes, F.; Oliveira, J.M.A.; Valentão, P.; Pereira, J.A.; Andrade, P.B. (2009). Metabolic profiling and biological capacity of Pieris brassicae fed with kale (Brassica oleracea L. var. acephala). Food and Chemical Toxicology. ISSN 0278-6915. 47:6, p.1209-12200278-691510.1016/j.fct.2009.02.014info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:07:59Zoai:bibliotecadigital.ipb.pt:10198/3103Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:55:59.512568Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Metabolic profiling and biological capacity of Pieris brassicae fed with kale (Brassica oleracea L. var. acephala). |
title |
Metabolic profiling and biological capacity of Pieris brassicae fed with kale (Brassica oleracea L. var. acephala). |
spellingShingle |
Metabolic profiling and biological capacity of Pieris brassicae fed with kale (Brassica oleracea L. var. acephala). Ferreres, Frederico Pieris brassicae L. Brassica oleracea L. var. acephala Phenolic compounds Organic acids Biological activity |
title_short |
Metabolic profiling and biological capacity of Pieris brassicae fed with kale (Brassica oleracea L. var. acephala). |
title_full |
Metabolic profiling and biological capacity of Pieris brassicae fed with kale (Brassica oleracea L. var. acephala). |
title_fullStr |
Metabolic profiling and biological capacity of Pieris brassicae fed with kale (Brassica oleracea L. var. acephala). |
title_full_unstemmed |
Metabolic profiling and biological capacity of Pieris brassicae fed with kale (Brassica oleracea L. var. acephala). |
title_sort |
Metabolic profiling and biological capacity of Pieris brassicae fed with kale (Brassica oleracea L. var. acephala). |
author |
Ferreres, Frederico |
author_facet |
Ferreres, Frederico Fernandes, Fátima Oliveira, Jorge M.A. Valentão, Patrícia Pereira, J.A. Andrade, Paula B. |
author_role |
author |
author2 |
Fernandes, Fátima Oliveira, Jorge M.A. Valentão, Patrícia Pereira, J.A. Andrade, Paula B. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Biblioteca Digital do IPB |
dc.contributor.author.fl_str_mv |
Ferreres, Frederico Fernandes, Fátima Oliveira, Jorge M.A. Valentão, Patrícia Pereira, J.A. Andrade, Paula B. |
dc.subject.por.fl_str_mv |
Pieris brassicae L. Brassica oleracea L. var. acephala Phenolic compounds Organic acids Biological activity |
topic |
Pieris brassicae L. Brassica oleracea L. var. acephala Phenolic compounds Organic acids Biological activity |
description |
Phenolic and organic acid profiles of aqueous extracts from Pieris brassicae material and the host kale (Brassica oleracea L. var. acephala) leaves were determined by HPLC/UV–DAD/MSn-ESI and HPLC–UV, respectively. The identified phenolics included acylated and nonacylated flavonoid glycosides, hydroxycinnamic acyl gentiobiosides, and sulphate phenolics. Kale exhibited the highest content (11 g/kg lyophilized extract), while no phenolics were identified in the butterflies or exuviae. Nine different organic acids were characterized in the materials, with kale showing the highest amount (112 g/kg lyophilized extract). With the exception of the exuviae extract, the rest were screened for bioactivity. Using spectrophotometric microassays, all exhibited antiradical capacity against DPPH and NO in a concentrationdependent way, whereas only kale and excrement extracts were active against superoxide. All displayed activity on intestinal smooth muscle, albeit with distinct relaxation–contraction profiles. Larvae and butterfly extracts were more efficacious for intestinal relaxation than was kale extract, whereas excrement extract evoked only contractions, thus evidencing their different compositions. Collectively, these results show that P. brassicae sequesters and metabolizes kale’s phenolic compounds. Moreover, the extract’s bioactivities suggest that they may constitute an interesting source of bioactive compounds whose complex chemical structures preclude either synthesis or isolation. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009 2009-01-01T00:00:00Z 2011-01-13T15:46:42Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10198/3103 |
url |
http://hdl.handle.net/10198/3103 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Ferreres, F.; Fernandes, F.; Oliveira, J.M.A.; Valentão, P.; Pereira, J.A.; Andrade, P.B. (2009). Metabolic profiling and biological capacity of Pieris brassicae fed with kale (Brassica oleracea L. var. acephala). Food and Chemical Toxicology. ISSN 0278-6915. 47:6, p.1209-1220 0278-6915 10.1016/j.fct.2009.02.014 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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