Flavones, flavonols, and glycosylated derivatives—impact on candida albicans growth and virulence, expression of cdr1 and erg11, cytotoxicity
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10198/24281 |
Resumo: | Due to the high incidence of fungal infections worldwide, there is an increasing demand for the development of novel therapeutic approaches. A wide range of natural products has been extensively studied, with considerable focus on flavonoids. The antifungal capacity of selected flavones (luteolin, apigenin), flavonols (quercetin), and their glycosylated derivatives (quercitrin, isoquercitrin, rutin, and apigetrin) along with their impact on genes encoding efflux pumps (CDR1) and ergosterol biosynthesis enzyme (ERG11) has been the subject of this study. Cytotoxi-city of flavonoids towards primary liver cells has also been addressed. Luteolin, quercitrin, isoquercitrin, and rutin inhibited growth of Candida albicans with the minimal inhibitory concentration of 37.5 µg/mL. The application of isoquercitrin has reduced C. albicans biofilm establishing capacities for 76%, and hyphal formation by yeast. In vitro treatment with apigenin, apigetrin, and quercitrin has downregulated CDR1. Contrary to rutin and apigenin, isoquercitrin has upregulated ERG11. Except apigetrin and quercitrin (90 µg/mL and 73 µg/mL, respectively inhibited 50% of the net cell growth), the examined flavonoids did not exhibit cytotoxicity. The reduction of both fungal virulence and expression of antifungal resistance-linked genes was the most pronounced for apigenin and apigetrin; these results indicate flavonoids’ indispensable capacity for further development as part of an anticandidal therapy or prevention strategy. |
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Flavones, flavonols, and glycosylated derivatives—impact on candida albicans growth and virulence, expression of cdr1 and erg11, cytotoxicityAntifungalAntivirulenceBiofilmCytotoxicityEfflux pumpsFlavonoidsIsoquercitrinDue to the high incidence of fungal infections worldwide, there is an increasing demand for the development of novel therapeutic approaches. A wide range of natural products has been extensively studied, with considerable focus on flavonoids. The antifungal capacity of selected flavones (luteolin, apigenin), flavonols (quercetin), and their glycosylated derivatives (quercitrin, isoquercitrin, rutin, and apigetrin) along with their impact on genes encoding efflux pumps (CDR1) and ergosterol biosynthesis enzyme (ERG11) has been the subject of this study. Cytotoxi-city of flavonoids towards primary liver cells has also been addressed. Luteolin, quercitrin, isoquercitrin, and rutin inhibited growth of Candida albicans with the minimal inhibitory concentration of 37.5 µg/mL. The application of isoquercitrin has reduced C. albicans biofilm establishing capacities for 76%, and hyphal formation by yeast. In vitro treatment with apigenin, apigetrin, and quercitrin has downregulated CDR1. Contrary to rutin and apigenin, isoquercitrin has upregulated ERG11. Except apigetrin and quercitrin (90 µg/mL and 73 µg/mL, respectively inhibited 50% of the net cell growth), the examined flavonoids did not exhibit cytotoxicity. The reduction of both fungal virulence and expression of antifungal resistance-linked genes was the most pronounced for apigenin and apigetrin; these results indicate flavonoids’ indispensable capacity for further development as part of an anticandidal therapy or prevention strategy.This research is funded by the Serbian Ministry of Education, Science and Technological Development [Contract No. 451-03-68/2020-14/200007]. The authors are grateful to the FEMS for providing FEMS Research and Training Grant [FEMS-GO-2017-015] to Marija Ivanov for her visit to Institute of Microbiology, University Hospital Lausanne and University Hospital Center, Rue du Bugnon 48, Lausanne, Switzerland. The authors are also grateful to the Foundation for Science and Technology (FCT, Portugal) for financial support through national funds FCT/MCTES to CIMO [UIDB/00690/2020] and to the national funding by FCT, P.I., through the institutional scientific employment program-contract for R. Calhelha’s contract.Biblioteca Digital do IPBIvanov, MarijaKannan, AbhilashStojković, DejanGlamočlija, JasminaCalhelha, Ricardo C.Ferreira, Isabel C.F.R.Sanglard, DominiqueSoković, Marina2018-01-19T10:00:00Z20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/24281engIvanov, Marija; Kannan, Abhilash; Stojković, Dejan S.; Glamočlija, Jasmina; Calhelha, Ricardo C.; Ferreira, Isabel C.F.R.; Sanglard, Dominique; Soković, Marina (2021). Flavones, flavonols, and glycosylated derivatives—impact on candida albicans growth and virulence, expression of cdr1 and erg11, cytotoxicity. Pharmaceuticals. ISSN 1424-8247. 14:1, p. 1-1210.3390/ph14010027info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:54:17Zoai:bibliotecadigital.ipb.pt:10198/24281Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:15:07.722802Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Flavones, flavonols, and glycosylated derivatives—impact on candida albicans growth and virulence, expression of cdr1 and erg11, cytotoxicity |
title |
Flavones, flavonols, and glycosylated derivatives—impact on candida albicans growth and virulence, expression of cdr1 and erg11, cytotoxicity |
spellingShingle |
Flavones, flavonols, and glycosylated derivatives—impact on candida albicans growth and virulence, expression of cdr1 and erg11, cytotoxicity Ivanov, Marija Antifungal Antivirulence Biofilm Cytotoxicity Efflux pumps Flavonoids Isoquercitrin |
title_short |
Flavones, flavonols, and glycosylated derivatives—impact on candida albicans growth and virulence, expression of cdr1 and erg11, cytotoxicity |
title_full |
Flavones, flavonols, and glycosylated derivatives—impact on candida albicans growth and virulence, expression of cdr1 and erg11, cytotoxicity |
title_fullStr |
Flavones, flavonols, and glycosylated derivatives—impact on candida albicans growth and virulence, expression of cdr1 and erg11, cytotoxicity |
title_full_unstemmed |
Flavones, flavonols, and glycosylated derivatives—impact on candida albicans growth and virulence, expression of cdr1 and erg11, cytotoxicity |
title_sort |
Flavones, flavonols, and glycosylated derivatives—impact on candida albicans growth and virulence, expression of cdr1 and erg11, cytotoxicity |
author |
Ivanov, Marija |
author_facet |
Ivanov, Marija Kannan, Abhilash Stojković, Dejan Glamočlija, Jasmina Calhelha, Ricardo C. Ferreira, Isabel C.F.R. Sanglard, Dominique Soković, Marina |
author_role |
author |
author2 |
Kannan, Abhilash Stojković, Dejan Glamočlija, Jasmina Calhelha, Ricardo C. Ferreira, Isabel C.F.R. Sanglard, Dominique Soković, Marina |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Biblioteca Digital do IPB |
dc.contributor.author.fl_str_mv |
Ivanov, Marija Kannan, Abhilash Stojković, Dejan Glamočlija, Jasmina Calhelha, Ricardo C. Ferreira, Isabel C.F.R. Sanglard, Dominique Soković, Marina |
dc.subject.por.fl_str_mv |
Antifungal Antivirulence Biofilm Cytotoxicity Efflux pumps Flavonoids Isoquercitrin |
topic |
Antifungal Antivirulence Biofilm Cytotoxicity Efflux pumps Flavonoids Isoquercitrin |
description |
Due to the high incidence of fungal infections worldwide, there is an increasing demand for the development of novel therapeutic approaches. A wide range of natural products has been extensively studied, with considerable focus on flavonoids. The antifungal capacity of selected flavones (luteolin, apigenin), flavonols (quercetin), and their glycosylated derivatives (quercitrin, isoquercitrin, rutin, and apigetrin) along with their impact on genes encoding efflux pumps (CDR1) and ergosterol biosynthesis enzyme (ERG11) has been the subject of this study. Cytotoxi-city of flavonoids towards primary liver cells has also been addressed. Luteolin, quercitrin, isoquercitrin, and rutin inhibited growth of Candida albicans with the minimal inhibitory concentration of 37.5 µg/mL. The application of isoquercitrin has reduced C. albicans biofilm establishing capacities for 76%, and hyphal formation by yeast. In vitro treatment with apigenin, apigetrin, and quercitrin has downregulated CDR1. Contrary to rutin and apigenin, isoquercitrin has upregulated ERG11. Except apigetrin and quercitrin (90 µg/mL and 73 µg/mL, respectively inhibited 50% of the net cell growth), the examined flavonoids did not exhibit cytotoxicity. The reduction of both fungal virulence and expression of antifungal resistance-linked genes was the most pronounced for apigenin and apigetrin; these results indicate flavonoids’ indispensable capacity for further development as part of an anticandidal therapy or prevention strategy. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01-19T10:00:00Z 2021 2021-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10198/24281 |
url |
http://hdl.handle.net/10198/24281 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Ivanov, Marija; Kannan, Abhilash; Stojković, Dejan S.; Glamočlija, Jasmina; Calhelha, Ricardo C.; Ferreira, Isabel C.F.R.; Sanglard, Dominique; Soković, Marina (2021). Flavones, flavonols, and glycosylated derivatives—impact on candida albicans growth and virulence, expression of cdr1 and erg11, cytotoxicity. Pharmaceuticals. ISSN 1424-8247. 14:1, p. 1-12 10.3390/ph14010027 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
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