Antiproliferative activity of neem leaf extracts obtained by a sequential pressurized liquid extraction
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10198/18725 |
Resumo: | Azadirachta indica A. Juss (neem) extracts have been used in pharmaceutical applications as antitumor agents, due to their terpenes and phenolic compounds. To obtain extracts from neem leaves with potential antiproliferative effect, a sequential process of pressurized liquid extraction was carried out in a fixed bed extractor at 25◦C and 100 bar, using hexane (SH), ethyl acetate (SEA), and ethanol (SE)assolvents. Extractions using only ethanol(EE) was also conducted to compare the characteristics of the fractionated extracts. The results obtained by liquid chromatography-electrospray ionization mass spectrometry suggested a higher concentration of terpenes in the SEA extract in comparison to SH, SE, and EE extracts. Therefore, antiproliferative activity showed that SEA extracts were the most efficient inhibitor to human tumor cells MCF-7, NCI-H460, HeLa, and HepG2. Hepatocellular cells were more resistant to SH, SEA, SE, and EE compared to breast, lung, hepatocellular, and cervical malignant cells. Neem fractioned extracts obtained in the present study seem to be more selective for malignant cells compared to the non-tumor cells. |
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Antiproliferative activity of neem leaf extracts obtained by a sequential pressurized liquid extractionAntiproliferative activityNeem leavesSequential pressurized liquid extractionAzadirachta indica A. Juss (neem) extracts have been used in pharmaceutical applications as antitumor agents, due to their terpenes and phenolic compounds. To obtain extracts from neem leaves with potential antiproliferative effect, a sequential process of pressurized liquid extraction was carried out in a fixed bed extractor at 25◦C and 100 bar, using hexane (SH), ethyl acetate (SEA), and ethanol (SE)assolvents. Extractions using only ethanol(EE) was also conducted to compare the characteristics of the fractionated extracts. The results obtained by liquid chromatography-electrospray ionization mass spectrometry suggested a higher concentration of terpenes in the SEA extract in comparison to SH, SE, and EE extracts. Therefore, antiproliferative activity showed that SEA extracts were the most efficient inhibitor to human tumor cells MCF-7, NCI-H460, HeLa, and HepG2. Hepatocellular cells were more resistant to SH, SEA, SE, and EE compared to breast, lung, hepatocellular, and cervical malignant cells. Neem fractioned extracts obtained in the present study seem to be more selective for malignant cells compared to the non-tumor cells.Klebson Silva Santos thanks CAPES (Process: PDSE 99999.003409/15-5) for the financial support during his Ph.D. studies in Portugal. Authors are grateful to CAPES, and FAPITEC (EDITAL CAPES/FAPITEC Nº 11/2016–PROEF/Processo de AUXPE 88881.157437/2017-01) for the financial support. The study was also carried out with financial support from FEDER, under the Partnership Agreement PT2020.Biblioteca Digital do IPBSantos, Klebson S.Barbosa, Andriele M.Freitas, VictorMuniz, Ana Veruska C.S.Mendonça, Marcelo C.Calhelha, Ricardo C.Ferreira, Isabel C.F.R.Franceschi, EltonPadilha, Francine F.Oliveira, BeatrizDariva, Cláudio2018-01-19T10:00:00Z20182018-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/18725engSantos, Klebson S.; Barbosa, Andriele M.; Freitas, Victor; Muniz, Ana Veruska C.S.; Mendonça, Marcelo C.; Calhelha, Ricardo C.; Ferreira, Isabel C.F.R.; Franceschi, Elton; Padilha, Francine F.; Oliveira, M.B.P.P.; Dariva, Cláudio (2018). Antiproliferative activity of neem leaf extracts obtained by a sequential pressurized liquid extraction. Pharmaceuticals. ISSN 14248247. 11:310.3390/ph11030076info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:42:55Zoai:bibliotecadigital.ipb.pt:10198/18725Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:09:04.345208Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Antiproliferative activity of neem leaf extracts obtained by a sequential pressurized liquid extraction |
title |
Antiproliferative activity of neem leaf extracts obtained by a sequential pressurized liquid extraction |
spellingShingle |
Antiproliferative activity of neem leaf extracts obtained by a sequential pressurized liquid extraction Santos, Klebson S. Antiproliferative activity Neem leaves Sequential pressurized liquid extraction |
title_short |
Antiproliferative activity of neem leaf extracts obtained by a sequential pressurized liquid extraction |
title_full |
Antiproliferative activity of neem leaf extracts obtained by a sequential pressurized liquid extraction |
title_fullStr |
Antiproliferative activity of neem leaf extracts obtained by a sequential pressurized liquid extraction |
title_full_unstemmed |
Antiproliferative activity of neem leaf extracts obtained by a sequential pressurized liquid extraction |
title_sort |
Antiproliferative activity of neem leaf extracts obtained by a sequential pressurized liquid extraction |
author |
Santos, Klebson S. |
author_facet |
Santos, Klebson S. Barbosa, Andriele M. Freitas, Victor Muniz, Ana Veruska C.S. Mendonça, Marcelo C. Calhelha, Ricardo C. Ferreira, Isabel C.F.R. Franceschi, Elton Padilha, Francine F. Oliveira, Beatriz Dariva, Cláudio |
author_role |
author |
author2 |
Barbosa, Andriele M. Freitas, Victor Muniz, Ana Veruska C.S. Mendonça, Marcelo C. Calhelha, Ricardo C. Ferreira, Isabel C.F.R. Franceschi, Elton Padilha, Francine F. Oliveira, Beatriz Dariva, Cláudio |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Biblioteca Digital do IPB |
dc.contributor.author.fl_str_mv |
Santos, Klebson S. Barbosa, Andriele M. Freitas, Victor Muniz, Ana Veruska C.S. Mendonça, Marcelo C. Calhelha, Ricardo C. Ferreira, Isabel C.F.R. Franceschi, Elton Padilha, Francine F. Oliveira, Beatriz Dariva, Cláudio |
dc.subject.por.fl_str_mv |
Antiproliferative activity Neem leaves Sequential pressurized liquid extraction |
topic |
Antiproliferative activity Neem leaves Sequential pressurized liquid extraction |
description |
Azadirachta indica A. Juss (neem) extracts have been used in pharmaceutical applications as antitumor agents, due to their terpenes and phenolic compounds. To obtain extracts from neem leaves with potential antiproliferative effect, a sequential process of pressurized liquid extraction was carried out in a fixed bed extractor at 25◦C and 100 bar, using hexane (SH), ethyl acetate (SEA), and ethanol (SE)assolvents. Extractions using only ethanol(EE) was also conducted to compare the characteristics of the fractionated extracts. The results obtained by liquid chromatography-electrospray ionization mass spectrometry suggested a higher concentration of terpenes in the SEA extract in comparison to SH, SE, and EE extracts. Therefore, antiproliferative activity showed that SEA extracts were the most efficient inhibitor to human tumor cells MCF-7, NCI-H460, HeLa, and HepG2. Hepatocellular cells were more resistant to SH, SEA, SE, and EE compared to breast, lung, hepatocellular, and cervical malignant cells. Neem fractioned extracts obtained in the present study seem to be more selective for malignant cells compared to the non-tumor cells. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01-19T10:00:00Z 2018 2018-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10198/18725 |
url |
http://hdl.handle.net/10198/18725 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Santos, Klebson S.; Barbosa, Andriele M.; Freitas, Victor; Muniz, Ana Veruska C.S.; Mendonça, Marcelo C.; Calhelha, Ricardo C.; Ferreira, Isabel C.F.R.; Franceschi, Elton; Padilha, Francine F.; Oliveira, M.B.P.P.; Dariva, Cláudio (2018). Antiproliferative activity of neem leaf extracts obtained by a sequential pressurized liquid extraction. Pharmaceuticals. ISSN 14248247. 11:3 10.3390/ph11030076 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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