Glutamine and cystine-enriched diets modulate aquaporins gene expression in the small intestine of piglets

Detalhes bibliográficos
Autor(a) principal: da Silva, Inês V.
Data de Publicação: 2021
Outros Autores: Soares, Bárbara P., Pimpão, Catarina, Pinto, Rui M. A., Costa, Teresa, Freire, João P. B., Corrent, Etienne, Chalvon-Demersay, Tristan, Prates, José A.M, Lopes, Paula A., Soveral, Graça
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/23318
Resumo: Research Areas: Science & Technology ; Other Topics
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spelling Glutamine and cystine-enriched diets modulate aquaporins gene expression in the small intestine of pigletsBasolateral membrane-vesiclesBrush-borderWater channelPermeabilityGlutathionePlasmaPigsSupplementationMetabolismDiseaseResearch Areas: Science & Technology ; Other TopicsABSTRACT - The regulation of glycerol permeability in the gastrointestinal tract is crucial to control fat deposition, lipolysis and gluconeogenesis. Knowing that the amino acid glutamine is a physiological regulator of gluconeogenesis, whereas cystine promotes adiposity, herein we investigated the effects of dietary supplementation with glutamine and cystine on the serum biochemical parameters of piglets fed on amino acid-enriched diets, as well as on the transcriptional profile of membrane water and glycerol channels aquaporins (AQPs) in the ileum portion of the small intestine and its impact on intestinal permeability. Twenty male piglets with an initial body weight of 8.8 ± 0.89 kg were allocated to four dietary treatments (n = 5) and received, during a four week-period, a basal diet without supplementation (control) or supplemented with 8 kg/ton of glutamine (Gln), cystine (Cys) or the combination of the two amino acids in equal proportions (Gln + Cys). Most biochemical parameters were found improved in piglets fed Gln and Cys diet. mRNA levels of AQP3 were found predominant over the others. Both amino acids, individually or combined, were responsible for a consistent downregulation of AQP1, AQP7 and AQP10, without impacting on water permeability. Conversely, Cys enriched diet upregulated AQP3 enhancing basolateral membranes glycerol permeability and downregulating glycerol kinase (GK) of intestinal cells. Altogether, our data reveal that amino acids dietary supplementation can modulate intestinal AQPs expression and unveil AQP3 as a promising target for adipogenesis regulation.Public Library ScienceRepositório da Universidade de Lisboada Silva, Inês V.Soares, Bárbara P.Pimpão, CatarinaPinto, Rui M. A.Costa, TeresaFreire, João P. B.Corrent, EtienneChalvon-Demersay, TristanPrates, José A.MLopes, Paula A.Soveral, Graça2022-01-30T22:52:12Z2021-01-192021-01-19T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/23318engDa Silva IV, Soares BP, Pimpão C, Pinto RMA, Costa T, Freire JPB, Corrent E, Chalvon-Demersay T, Prates JAM, et al. 2021. Glutamine and cystine-enriched diets modulate aquaporins gene expression in the small intestine of piglets. PLoS ONE 16(1):e0245739. Doi: 10.1371/journal.pone.02457391932-620310.1371/journal.pone.0245739info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:52:50ZPortal AgregadorONG
dc.title.none.fl_str_mv Glutamine and cystine-enriched diets modulate aquaporins gene expression in the small intestine of piglets
title Glutamine and cystine-enriched diets modulate aquaporins gene expression in the small intestine of piglets
spellingShingle Glutamine and cystine-enriched diets modulate aquaporins gene expression in the small intestine of piglets
da Silva, Inês V.
Basolateral membrane-vesicles
Brush-border
Water channel
Permeability
Glutathione
Plasma
Pigs
Supplementation
Metabolism
Disease
title_short Glutamine and cystine-enriched diets modulate aquaporins gene expression in the small intestine of piglets
title_full Glutamine and cystine-enriched diets modulate aquaporins gene expression in the small intestine of piglets
title_fullStr Glutamine and cystine-enriched diets modulate aquaporins gene expression in the small intestine of piglets
title_full_unstemmed Glutamine and cystine-enriched diets modulate aquaporins gene expression in the small intestine of piglets
title_sort Glutamine and cystine-enriched diets modulate aquaporins gene expression in the small intestine of piglets
author da Silva, Inês V.
author_facet da Silva, Inês V.
Soares, Bárbara P.
Pimpão, Catarina
Pinto, Rui M. A.
Costa, Teresa
Freire, João P. B.
Corrent, Etienne
Chalvon-Demersay, Tristan
Prates, José A.M
Lopes, Paula A.
Soveral, Graça
author_role author
author2 Soares, Bárbara P.
Pimpão, Catarina
Pinto, Rui M. A.
Costa, Teresa
Freire, João P. B.
Corrent, Etienne
Chalvon-Demersay, Tristan
Prates, José A.M
Lopes, Paula A.
Soveral, Graça
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv da Silva, Inês V.
Soares, Bárbara P.
Pimpão, Catarina
Pinto, Rui M. A.
Costa, Teresa
Freire, João P. B.
Corrent, Etienne
Chalvon-Demersay, Tristan
Prates, José A.M
Lopes, Paula A.
Soveral, Graça
dc.subject.por.fl_str_mv Basolateral membrane-vesicles
Brush-border
Water channel
Permeability
Glutathione
Plasma
Pigs
Supplementation
Metabolism
Disease
topic Basolateral membrane-vesicles
Brush-border
Water channel
Permeability
Glutathione
Plasma
Pigs
Supplementation
Metabolism
Disease
description Research Areas: Science & Technology ; Other Topics
publishDate 2021
dc.date.none.fl_str_mv 2021-01-19
2021-01-19T00:00:00Z
2022-01-30T22:52:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/23318
url http://hdl.handle.net/10400.5/23318
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Da Silva IV, Soares BP, Pimpão C, Pinto RMA, Costa T, Freire JPB, Corrent E, Chalvon-Demersay T, Prates JAM, et al. 2021. Glutamine and cystine-enriched diets modulate aquaporins gene expression in the small intestine of piglets. PLoS ONE 16(1):e0245739. Doi: 10.1371/journal.pone.0245739
1932-6203
10.1371/journal.pone.0245739
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Public Library Science
publisher.none.fl_str_mv Public Library Science
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv
repository.mail.fl_str_mv
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