Polymerase chain reaction-based method for the identification of leishmania (viannia) Braziliensis and leishmania (viannia) guyanensis in mucosal tissues conserved in paraffin

Detalhes bibliográficos
Autor(a) principal: Prestes, Suzane Ribeiro
Data de Publicação: 2015
Outros Autores: Guerra, Jorge Augusto de Oliveira, Romero, Gustavo Adolfo Sierra, Magalhaes, Laylah Kelre Costa, Santana, Rosa Amelia Gonçalves, Maciel, Marcel Gonçalves, Custódio, Ana, Barbosa, Maria das Graças Vale, Silveira, Henrique
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://doi.org/10.1590/0037-8682-0132-2015
Resumo: WOS:000364431200008
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spelling Polymerase chain reaction-based method for the identification of leishmania (viannia) Braziliensis and leishmania (viannia) guyanensis in mucosal tissues conserved in paraffinLeishmania BraziliensisLeishmania guyanensisMolecular diagnosisMucosal leishmaniasisPCRInfectious DiseasesParasitologyGeneticsSDG 3 - Good Health and Well-beingWOS:000364431200008Introduction: In the Americas, mucosal leishmaniasis is primarily associated with infection by Leishmania (Viannia) braziliensis. However, Leishmania (Viannia) guyanensis is another important cause of this disease in the Brazilian Amazon. In this study, we aimed at detecting Leishmania deoxyribonucleic acid (DNA) within paraffi n-embedded fragments of mucosal tissues, and characterizing the infecting parasite species. Methods: We evaluated samples collected from 114 patients treated at a reference center in the Brazilian Amazon by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analyses. Results: Direct examination of biopsy imprints detected parasites in 10 of the 114 samples, while evaluation of hematoxylin and eosin-stained slides detected amastigotes in an additional 17 samples. Meanwhile, 31/114 samples (27.2%) were positive for Leishmania spp. kinetoplast deoxyribonucleic acid (kDNA) by PCR analysis. Of these, 17 (54.8%) yielded amplifi cation of the mini-exon PCR target, thereby allowing for PCR-RFLP-based identifi cation. Six of the samples were identifi ed as L. (V.) braziliensis, while the remaining 11 were identifi ed as L. (V.) guyanensis. Conclusions: The results of this study demonstrate the feasibility of applying molecular techniques for the diagnosis of human parasites within paraffi nembedded tissues. Moreover, our fi ndings confi rm that L. (V.) guyanensis is a relevant causative agent of mucosal leishmaniasis in the Brazilian Amazon.Instituto de Higiene e Medicina Tropical (IHMT)Global Health and Tropical Medicine (GHTM)Vector borne diseases and pathogens (VBD)RUNPrestes, Suzane RibeiroGuerra, Jorge Augusto de OliveiraRomero, Gustavo Adolfo SierraMagalhaes, Laylah Kelre CostaSantana, Rosa Amelia GonçalvesMaciel, Marcel GonçalvesCustódio, AnaBarbosa, Maria das Graças ValeSilveira, Henrique2018-05-11T22:01:59Z2015-09-012015-09-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article5application/pdfhttps://doi.org/10.1590/0037-8682-0132-2015eng0037-8682PURE: 1179380http://www.scopus.com/inward/record.url?scp=84947435381&partnerID=8YFLogxKhttps://doi.org/10.1590/0037-8682-0132-2015info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:20:02Zoai:run.unl.pt:10362/36575Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:30:33.915932Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Polymerase chain reaction-based method for the identification of leishmania (viannia) Braziliensis and leishmania (viannia) guyanensis in mucosal tissues conserved in paraffin
title Polymerase chain reaction-based method for the identification of leishmania (viannia) Braziliensis and leishmania (viannia) guyanensis in mucosal tissues conserved in paraffin
spellingShingle Polymerase chain reaction-based method for the identification of leishmania (viannia) Braziliensis and leishmania (viannia) guyanensis in mucosal tissues conserved in paraffin
Prestes, Suzane Ribeiro
Leishmania Braziliensis
Leishmania guyanensis
Molecular diagnosis
Mucosal leishmaniasis
PCR
Infectious Diseases
Parasitology
Genetics
SDG 3 - Good Health and Well-being
title_short Polymerase chain reaction-based method for the identification of leishmania (viannia) Braziliensis and leishmania (viannia) guyanensis in mucosal tissues conserved in paraffin
title_full Polymerase chain reaction-based method for the identification of leishmania (viannia) Braziliensis and leishmania (viannia) guyanensis in mucosal tissues conserved in paraffin
title_fullStr Polymerase chain reaction-based method for the identification of leishmania (viannia) Braziliensis and leishmania (viannia) guyanensis in mucosal tissues conserved in paraffin
title_full_unstemmed Polymerase chain reaction-based method for the identification of leishmania (viannia) Braziliensis and leishmania (viannia) guyanensis in mucosal tissues conserved in paraffin
title_sort Polymerase chain reaction-based method for the identification of leishmania (viannia) Braziliensis and leishmania (viannia) guyanensis in mucosal tissues conserved in paraffin
author Prestes, Suzane Ribeiro
author_facet Prestes, Suzane Ribeiro
Guerra, Jorge Augusto de Oliveira
Romero, Gustavo Adolfo Sierra
Magalhaes, Laylah Kelre Costa
Santana, Rosa Amelia Gonçalves
Maciel, Marcel Gonçalves
Custódio, Ana
Barbosa, Maria das Graças Vale
Silveira, Henrique
author_role author
author2 Guerra, Jorge Augusto de Oliveira
Romero, Gustavo Adolfo Sierra
Magalhaes, Laylah Kelre Costa
Santana, Rosa Amelia Gonçalves
Maciel, Marcel Gonçalves
Custódio, Ana
Barbosa, Maria das Graças Vale
Silveira, Henrique
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Higiene e Medicina Tropical (IHMT)
Global Health and Tropical Medicine (GHTM)
Vector borne diseases and pathogens (VBD)
RUN
dc.contributor.author.fl_str_mv Prestes, Suzane Ribeiro
Guerra, Jorge Augusto de Oliveira
Romero, Gustavo Adolfo Sierra
Magalhaes, Laylah Kelre Costa
Santana, Rosa Amelia Gonçalves
Maciel, Marcel Gonçalves
Custódio, Ana
Barbosa, Maria das Graças Vale
Silveira, Henrique
dc.subject.por.fl_str_mv Leishmania Braziliensis
Leishmania guyanensis
Molecular diagnosis
Mucosal leishmaniasis
PCR
Infectious Diseases
Parasitology
Genetics
SDG 3 - Good Health and Well-being
topic Leishmania Braziliensis
Leishmania guyanensis
Molecular diagnosis
Mucosal leishmaniasis
PCR
Infectious Diseases
Parasitology
Genetics
SDG 3 - Good Health and Well-being
description WOS:000364431200008
publishDate 2015
dc.date.none.fl_str_mv 2015-09-01
2015-09-01T00:00:00Z
2018-05-11T22:01:59Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://doi.org/10.1590/0037-8682-0132-2015
url https://doi.org/10.1590/0037-8682-0132-2015
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0037-8682
PURE: 1179380
http://www.scopus.com/inward/record.url?scp=84947435381&partnerID=8YFLogxK
https://doi.org/10.1590/0037-8682-0132-2015
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 5
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dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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