A membrane bioreactor for biotransformations of hydrophobic molecules using organic solvent nanofiltration (OSN) membranes
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.14/2719 |
Resumo: | Thiswork reports the application of organic solvent nanofiltration (OSN) membranes to a membrane bioreactor for biotransformations (MBB). An organic solvent phasewas employed, allowing high substrate loadings and efficient product removal. The aqueous and organic phases were separated by an OSN membrane. The biotransformation of geraniol to R-citronellol by baker’s yeast was used as the model reaction, and n-hexadecane and toluene as the organic solvents. The performance of the MBB was compared to that of a direct contact biphasic (DCB) bioreactor. The MBB system resulted in lower productivities than the DCB system due to mass transfer limitations. For the n-hexadecane system, the membrane was the main mass transfer resistance, whereas for the toluene system the contribution of the aqueous liquid film mass transfer resistance became predominant. Further investigations are needed to improve the substrate transfer rates. Despite this, the MBB system prevented aqueous breakthrough, and thus the formation of two-phase emulsions. Toluene toxicity to the biocatalyst was also minimized, although it caused a reduction in the reaction enantiospecificity. This work showed that OSN-MBB systems avoid the formation of emulsions, thus reducing downstream separation and allowing increased substrate loadings. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
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A membrane bioreactor for biotransformations of hydrophobic molecules using organic solvent nanofiltration (OSN) membranesBiphasic membrane bioreactorEnantioselective biotransformationsOrganic solvent nanofiltration membranesMembrane extractionBaker’s yeastThiswork reports the application of organic solvent nanofiltration (OSN) membranes to a membrane bioreactor for biotransformations (MBB). An organic solvent phasewas employed, allowing high substrate loadings and efficient product removal. The aqueous and organic phases were separated by an OSN membrane. The biotransformation of geraniol to R-citronellol by baker’s yeast was used as the model reaction, and n-hexadecane and toluene as the organic solvents. The performance of the MBB was compared to that of a direct contact biphasic (DCB) bioreactor. The MBB system resulted in lower productivities than the DCB system due to mass transfer limitations. For the n-hexadecane system, the membrane was the main mass transfer resistance, whereas for the toluene system the contribution of the aqueous liquid film mass transfer resistance became predominant. Further investigations are needed to improve the substrate transfer rates. Despite this, the MBB system prevented aqueous breakthrough, and thus the formation of two-phase emulsions. Toluene toxicity to the biocatalyst was also minimized, although it caused a reduction in the reaction enantiospecificity. This work showed that OSN-MBB systems avoid the formation of emulsions, thus reducing downstream separation and allowing increased substrate loadings.ElsevierVeritati - Repositório Institucional da Universidade Católica PortuguesaValadez-Blanco, RogelioFerreira, Frederico CasteloJorge, Ruben FerreiraLivingston, Andrew Guy2010-10-08T14:32:06Z20082008-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/2719eng"Journal of Membrane Science". ISSN 0376-7388. 317: 1-2 (2008) 50–64info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-12T17:09:08Zoai:repositorio.ucp.pt:10400.14/2719Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:04:52.391545Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
A membrane bioreactor for biotransformations of hydrophobic molecules using organic solvent nanofiltration (OSN) membranes |
title |
A membrane bioreactor for biotransformations of hydrophobic molecules using organic solvent nanofiltration (OSN) membranes |
spellingShingle |
A membrane bioreactor for biotransformations of hydrophobic molecules using organic solvent nanofiltration (OSN) membranes Valadez-Blanco, Rogelio Biphasic membrane bioreactor Enantioselective biotransformations Organic solvent nanofiltration membranes Membrane extraction Baker’s yeast |
title_short |
A membrane bioreactor for biotransformations of hydrophobic molecules using organic solvent nanofiltration (OSN) membranes |
title_full |
A membrane bioreactor for biotransformations of hydrophobic molecules using organic solvent nanofiltration (OSN) membranes |
title_fullStr |
A membrane bioreactor for biotransformations of hydrophobic molecules using organic solvent nanofiltration (OSN) membranes |
title_full_unstemmed |
A membrane bioreactor for biotransformations of hydrophobic molecules using organic solvent nanofiltration (OSN) membranes |
title_sort |
A membrane bioreactor for biotransformations of hydrophobic molecules using organic solvent nanofiltration (OSN) membranes |
author |
Valadez-Blanco, Rogelio |
author_facet |
Valadez-Blanco, Rogelio Ferreira, Frederico Castelo Jorge, Ruben Ferreira Livingston, Andrew Guy |
author_role |
author |
author2 |
Ferreira, Frederico Castelo Jorge, Ruben Ferreira Livingston, Andrew Guy |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Veritati - Repositório Institucional da Universidade Católica Portuguesa |
dc.contributor.author.fl_str_mv |
Valadez-Blanco, Rogelio Ferreira, Frederico Castelo Jorge, Ruben Ferreira Livingston, Andrew Guy |
dc.subject.por.fl_str_mv |
Biphasic membrane bioreactor Enantioselective biotransformations Organic solvent nanofiltration membranes Membrane extraction Baker’s yeast |
topic |
Biphasic membrane bioreactor Enantioselective biotransformations Organic solvent nanofiltration membranes Membrane extraction Baker’s yeast |
description |
Thiswork reports the application of organic solvent nanofiltration (OSN) membranes to a membrane bioreactor for biotransformations (MBB). An organic solvent phasewas employed, allowing high substrate loadings and efficient product removal. The aqueous and organic phases were separated by an OSN membrane. The biotransformation of geraniol to R-citronellol by baker’s yeast was used as the model reaction, and n-hexadecane and toluene as the organic solvents. The performance of the MBB was compared to that of a direct contact biphasic (DCB) bioreactor. The MBB system resulted in lower productivities than the DCB system due to mass transfer limitations. For the n-hexadecane system, the membrane was the main mass transfer resistance, whereas for the toluene system the contribution of the aqueous liquid film mass transfer resistance became predominant. Further investigations are needed to improve the substrate transfer rates. Despite this, the MBB system prevented aqueous breakthrough, and thus the formation of two-phase emulsions. Toluene toxicity to the biocatalyst was also minimized, although it caused a reduction in the reaction enantiospecificity. This work showed that OSN-MBB systems avoid the formation of emulsions, thus reducing downstream separation and allowing increased substrate loadings. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008 2008-01-01T00:00:00Z 2010-10-08T14:32:06Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.14/2719 |
url |
http://hdl.handle.net/10400.14/2719 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
"Journal of Membrane Science". ISSN 0376-7388. 317: 1-2 (2008) 50–64 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
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1799131710736039936 |