Preliminary Studies Towards the Development of DNA Biosensors for Detection of Cylindrospermopsin - A Cyanobacterial Toxin

Detalhes bibliográficos
Autor(a) principal: Valério,E.
Data de Publicação: 2008
Outros Autores: Tenreiro,A., Abrantes,L.M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://scielo.pt/scielo.php?script=sci_arttext&pid=S0872-19042008000100006
Resumo: The suitability of polytyramine (PTy) modified electrodes for the development of DNA biosensors is analysed in this work. PTy films were synthesised potentiodynamically on Pt electrode, from acidic aqueous solution. A cylindrospermopsin coding sequence (PKSM4 probe 5’-phosphate modified) was covalently bound on the polytyramine matrix using N-hydroxysuccinimide (NHS) and 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC) as coupling agents. Changes of the open circuit potential were registered as a first approach to monitorise the immobilization and hybridization processes. The modified electrodes have been characterised by cyclic voltammetry and square wave voltammetry, using methylene blue (MB), as electroactive indicator and the successful immobilization and hybridization steps are revealed by the increase of the peak currents. The hybridization has also been confirmed by measuring the fluorescence of the modified electrodes after staining with PicoGreen® DNA dye.
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spelling Preliminary Studies Towards the Development of DNA Biosensors for Detection of Cylindrospermopsin - A Cyanobacterial ToxincylindrospermopsinDNA biosensorPicoGreen®polytyraminemethylene blueThe suitability of polytyramine (PTy) modified electrodes for the development of DNA biosensors is analysed in this work. PTy films were synthesised potentiodynamically on Pt electrode, from acidic aqueous solution. A cylindrospermopsin coding sequence (PKSM4 probe 5’-phosphate modified) was covalently bound on the polytyramine matrix using N-hydroxysuccinimide (NHS) and 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC) as coupling agents. Changes of the open circuit potential were registered as a first approach to monitorise the immobilization and hybridization processes. The modified electrodes have been characterised by cyclic voltammetry and square wave voltammetry, using methylene blue (MB), as electroactive indicator and the successful immobilization and hybridization steps are revealed by the increase of the peak currents. The hybridization has also been confirmed by measuring the fluorescence of the modified electrodes after staining with PicoGreen® DNA dye.Sociedade Portuguesa de Electroquímica2008-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articletext/htmlhttp://scielo.pt/scielo.php?script=sci_arttext&pid=S0872-19042008000100006Portugaliae Electrochimica Acta v.26 n.1 2008reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAPenghttp://scielo.pt/scielo.php?script=sci_arttext&pid=S0872-19042008000100006Valério,E.Tenreiro,A.Abrantes,L.M.info:eu-repo/semantics/openAccess2024-02-06T17:06:54Zoai:scielo:S0872-19042008000100006Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T02:20:03.686346Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Preliminary Studies Towards the Development of DNA Biosensors for Detection of Cylindrospermopsin - A Cyanobacterial Toxin
title Preliminary Studies Towards the Development of DNA Biosensors for Detection of Cylindrospermopsin - A Cyanobacterial Toxin
spellingShingle Preliminary Studies Towards the Development of DNA Biosensors for Detection of Cylindrospermopsin - A Cyanobacterial Toxin
Valério,E.
cylindrospermopsin
DNA biosensor
PicoGreen®
polytyramine
methylene blue
title_short Preliminary Studies Towards the Development of DNA Biosensors for Detection of Cylindrospermopsin - A Cyanobacterial Toxin
title_full Preliminary Studies Towards the Development of DNA Biosensors for Detection of Cylindrospermopsin - A Cyanobacterial Toxin
title_fullStr Preliminary Studies Towards the Development of DNA Biosensors for Detection of Cylindrospermopsin - A Cyanobacterial Toxin
title_full_unstemmed Preliminary Studies Towards the Development of DNA Biosensors for Detection of Cylindrospermopsin - A Cyanobacterial Toxin
title_sort Preliminary Studies Towards the Development of DNA Biosensors for Detection of Cylindrospermopsin - A Cyanobacterial Toxin
author Valério,E.
author_facet Valério,E.
Tenreiro,A.
Abrantes,L.M.
author_role author
author2 Tenreiro,A.
Abrantes,L.M.
author2_role author
author
dc.contributor.author.fl_str_mv Valério,E.
Tenreiro,A.
Abrantes,L.M.
dc.subject.por.fl_str_mv cylindrospermopsin
DNA biosensor
PicoGreen®
polytyramine
methylene blue
topic cylindrospermopsin
DNA biosensor
PicoGreen®
polytyramine
methylene blue
description The suitability of polytyramine (PTy) modified electrodes for the development of DNA biosensors is analysed in this work. PTy films were synthesised potentiodynamically on Pt electrode, from acidic aqueous solution. A cylindrospermopsin coding sequence (PKSM4 probe 5’-phosphate modified) was covalently bound on the polytyramine matrix using N-hydroxysuccinimide (NHS) and 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC) as coupling agents. Changes of the open circuit potential were registered as a first approach to monitorise the immobilization and hybridization processes. The modified electrodes have been characterised by cyclic voltammetry and square wave voltammetry, using methylene blue (MB), as electroactive indicator and the successful immobilization and hybridization steps are revealed by the increase of the peak currents. The hybridization has also been confirmed by measuring the fluorescence of the modified electrodes after staining with PicoGreen® DNA dye.
publishDate 2008
dc.date.none.fl_str_mv 2008-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://scielo.pt/scielo.php?script=sci_arttext&pid=S0872-19042008000100006
url http://scielo.pt/scielo.php?script=sci_arttext&pid=S0872-19042008000100006
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv http://scielo.pt/scielo.php?script=sci_arttext&pid=S0872-19042008000100006
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Portuguesa de Electroquímica
publisher.none.fl_str_mv Sociedade Portuguesa de Electroquímica
dc.source.none.fl_str_mv Portugaliae Electrochimica Acta v.26 n.1 2008
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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