Bioburden assessment by passive methods on a clinical pathology service in one central hospital from Lisbon: what can it tell us regarding patients and staff exposure?

Detalhes bibliográficos
Autor(a) principal: Viegas, Carla
Data de Publicação: 2020
Outros Autores: Twarużek, Magdalena, Lourenço, Raquel, Dias, Marta, Almeida, Beatriz, Aranha Caetano, Liliana, Carolino, Elisabete, Gomes, Anita Q., Kosicki, Robert, Soszczyńska, Ewelina, Viegas, Susana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.21/11415
Resumo: Project EXPOsE. Project nº 23222 (02/SAICT/2016).
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spelling Bioburden assessment by passive methods on a clinical pathology service in one central hospital from Lisbon: what can it tell us regarding patients and staff exposure?Occupational healthOccupational exposureBioburdenPassive methodsAspergillusAzole-resistance profileCytotoxicity analysisProject EXPOsEProject nº 23222 (02/SAICT/2016)PortugalLisboaProject EXPOsE. Project nº 23222 (02/SAICT/2016).The assessment and control of microbial contamination in healthcare facilities is presently a mandatory and vital part of strategies to prevent and control hospital-acquired infections. This study aims to assess the bioburden with two passive sampling methods (30 ventilations grids swabs and 16 electrostatic dust collectors (EDCs)) at Clinical Pathology Services. The fungal burden was characterized through molecular tools, antifungal resistance, and the mycotoxins and cytotoxicity profile. Total bacteria presented the highest prevalence in both matrixes, whereas Gram-bacteria presented the lowest. Swabs presented a higher prevalence (27.6%) for fungal burden. Chrysonilia sitophila presented the highest prevalence in swabs, whereas for EDCs, C. sitophila and Mucor sp. were the most prevalent. Concerning Aspergillus genera on swabs, section Flavi was the one with the highest prevalence (58.02%), whereas, for EDCs, section Versicolores was the only section observed (100%). Aspergillus section Fumigati was detected in 10 swabs and 7 EDC samples and Aspergillus section Versicolores were detected in one EDC sample. Fungal growth on azole-supplemented media was observed in eight EDC samples. No mycotoxins were detected in any of the samples. A low cytotoxic effect was observed in two sites upon incubation of collected samples with A549 and SK cells and in two other sites upon incubation of collected samples with SK cells only. A medium cytotoxic effect was observed with one EDC sample upon incubation with A549 cells. This study reinforces the need for determination of the azole resistance profile for fungal species and allowed a preliminary risk characterization regarding the cytotoxicity. An intervention including the use of ultraviolet with a wavelength between 200 nm and 280 nm (UVC)—emitting device and increased maintenance and cleaning of the central heating, ventilation, and air conditioning (HVAC) systems should be ensured to promote the reduction of microbial contamination.MDPIRCIPLViegas, CarlaTwarużek, MagdalenaLourenço, RaquelDias, MartaAlmeida, BeatrizAranha Caetano, LilianaCarolino, ElisabeteGomes, Anita Q.Kosicki, RobertSoszczyńska, EwelinaViegas, Susana2020-04-06T11:28:28Z2020-042020-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.21/11415engViegas C, Twarużek M, Caetano LA, Carolino E, Gomes AQ, Viegas S, et al. Bioburden assessment by passive methods on a clinical pathology service in one central hospital from Lisbon: what can it tell us regarding patients and staff exposure? Atmosphere. 2020;11(4):351.10.3390/atmos11040351info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-08-03T10:02:38ZPortal AgregadorONG
dc.title.none.fl_str_mv Bioburden assessment by passive methods on a clinical pathology service in one central hospital from Lisbon: what can it tell us regarding patients and staff exposure?
title Bioburden assessment by passive methods on a clinical pathology service in one central hospital from Lisbon: what can it tell us regarding patients and staff exposure?
spellingShingle Bioburden assessment by passive methods on a clinical pathology service in one central hospital from Lisbon: what can it tell us regarding patients and staff exposure?
Viegas, Carla
Occupational health
Occupational exposure
Bioburden
Passive methods
Aspergillus
Azole-resistance profile
Cytotoxicity analysis
Project EXPOsE
Project nº 23222 (02/SAICT/2016)
Portugal
Lisboa
title_short Bioburden assessment by passive methods on a clinical pathology service in one central hospital from Lisbon: what can it tell us regarding patients and staff exposure?
title_full Bioburden assessment by passive methods on a clinical pathology service in one central hospital from Lisbon: what can it tell us regarding patients and staff exposure?
title_fullStr Bioburden assessment by passive methods on a clinical pathology service in one central hospital from Lisbon: what can it tell us regarding patients and staff exposure?
title_full_unstemmed Bioburden assessment by passive methods on a clinical pathology service in one central hospital from Lisbon: what can it tell us regarding patients and staff exposure?
title_sort Bioburden assessment by passive methods on a clinical pathology service in one central hospital from Lisbon: what can it tell us regarding patients and staff exposure?
author Viegas, Carla
author_facet Viegas, Carla
Twarużek, Magdalena
Lourenço, Raquel
Dias, Marta
Almeida, Beatriz
Aranha Caetano, Liliana
Carolino, Elisabete
Gomes, Anita Q.
Kosicki, Robert
Soszczyńska, Ewelina
Viegas, Susana
author_role author
author2 Twarużek, Magdalena
Lourenço, Raquel
Dias, Marta
Almeida, Beatriz
Aranha Caetano, Liliana
Carolino, Elisabete
Gomes, Anita Q.
Kosicki, Robert
Soszczyńska, Ewelina
Viegas, Susana
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv RCIPL
dc.contributor.author.fl_str_mv Viegas, Carla
Twarużek, Magdalena
Lourenço, Raquel
Dias, Marta
Almeida, Beatriz
Aranha Caetano, Liliana
Carolino, Elisabete
Gomes, Anita Q.
Kosicki, Robert
Soszczyńska, Ewelina
Viegas, Susana
dc.subject.por.fl_str_mv Occupational health
Occupational exposure
Bioburden
Passive methods
Aspergillus
Azole-resistance profile
Cytotoxicity analysis
Project EXPOsE
Project nº 23222 (02/SAICT/2016)
Portugal
Lisboa
topic Occupational health
Occupational exposure
Bioburden
Passive methods
Aspergillus
Azole-resistance profile
Cytotoxicity analysis
Project EXPOsE
Project nº 23222 (02/SAICT/2016)
Portugal
Lisboa
description Project EXPOsE. Project nº 23222 (02/SAICT/2016).
publishDate 2020
dc.date.none.fl_str_mv 2020-04-06T11:28:28Z
2020-04
2020-04-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.21/11415
url http://hdl.handle.net/10400.21/11415
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Viegas C, Twarużek M, Caetano LA, Carolino E, Gomes AQ, Viegas S, et al. Bioburden assessment by passive methods on a clinical pathology service in one central hospital from Lisbon: what can it tell us regarding patients and staff exposure? Atmosphere. 2020;11(4):351.
10.3390/atmos11040351
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv
repository.mail.fl_str_mv
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