Skin byproducts of reinhardtius hippoglossoides (Greenland Halibut) as ecosustainable source of marine collagen
Autor(a) principal: | |
---|---|
Data de Publicação: | 2022 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/80564 |
Resumo: | Collagen is a ubiquitous protein present in the extracellular matrix of all major metazoan animals, with approximately 28 different human collagen types described in the literature, each with unique physicochemical properties. Collagens found broad application in the cosmeceutical, pharmaceutical, and biomedical fields and can be isolated from environmentally sustainable sources such as marine byproducts, which are abundant in the fish processing industry and are highly appealing low-cost sources. In this study, marine collagen was isolated from the skins of Greenland halibut (Reinhardtius hippoglossoides), an unexplored byproduct from fish processing plants, using three different collagen extraction methods, due to the use of distinct salting-out methods using a solution of 2.6 M NaCl + 0.05 M Tris-HCl pH = 7.5, (method I); a combination of 0.7 M NaCl followed by a solution of 2.3 M NaCl + 0.05 M Tris-HCl pH = 7.5 (method II); and one method using only 0.9 M NaCl (method III), yielding COLRp_I, COLRp_II, and COLRp_III collagens. These extracted type I collagens were produced with a yield of around 2 and 4% and characterized regarding the physicochemical properties, considering possible biotechnological applications. This work evidenced that the typical triple helix structure conformation was preserved in all extraction methods, but influenced the thermal behavior, intrinsic morphology, and moisture capacity of the collagens, with interest for biotechnological application, as the incorporation as an ingredient in cosmetic formulation. Furthermore, the use of collagen isolated from skin byproducts represents a high economic value with decreasing collagen cost for industrial purposes and is also an environmentally sustainable source for industrial uses. |
id |
RCAP_51f9e1c27ac865139a0aa9e23bc7db44 |
---|---|
oai_identifier_str |
oai:repositorium.sdum.uminho.pt:1822/80564 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Skin byproducts of reinhardtius hippoglossoides (Greenland Halibut) as ecosustainable source of marine collagenMarine collagensFish skinsByproduct valorizationActive ingredientCircular economyScience & TechnologyCollagen is a ubiquitous protein present in the extracellular matrix of all major metazoan animals, with approximately 28 different human collagen types described in the literature, each with unique physicochemical properties. Collagens found broad application in the cosmeceutical, pharmaceutical, and biomedical fields and can be isolated from environmentally sustainable sources such as marine byproducts, which are abundant in the fish processing industry and are highly appealing low-cost sources. In this study, marine collagen was isolated from the skins of Greenland halibut (Reinhardtius hippoglossoides), an unexplored byproduct from fish processing plants, using three different collagen extraction methods, due to the use of distinct salting-out methods using a solution of 2.6 M NaCl + 0.05 M Tris-HCl pH = 7.5, (method I); a combination of 0.7 M NaCl followed by a solution of 2.3 M NaCl + 0.05 M Tris-HCl pH = 7.5 (method II); and one method using only 0.9 M NaCl (method III), yielding COLRp_I, COLRp_II, and COLRp_III collagens. These extracted type I collagens were produced with a yield of around 2 and 4% and characterized regarding the physicochemical properties, considering possible biotechnological applications. This work evidenced that the typical triple helix structure conformation was preserved in all extraction methods, but influenced the thermal behavior, intrinsic morphology, and moisture capacity of the collagens, with interest for biotechnological application, as the incorporation as an ingredient in cosmetic formulation. Furthermore, the use of collagen isolated from skin byproducts represents a high economic value with decreasing collagen cost for industrial purposes and is also an environmentally sustainable source for industrial uses.This research was funded by the European Union Transborder Cooperation Programme Interreg Espana-Portugal 2014-2020 (POCTEP), within the European Regional Development Fund (ERDF), under the project 0302_CVMAR_I_1_P.MDPIUniversidade do MinhoMartins, EvaFernandes, RitaAlves, Ana L.Sousa, Rita O.Reis, R. L.Silva, Tiago H.2022-112022-11-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/80564engMartins E., Fernandes R., Alves A. L., Sousa R. O., Reis R. L., Silva T. H. Skin byproducts of reinhardtius hippoglossoides (Greenland Halibut) as ecosustainable source of marine collagen, Applied Sciences, Vol. 12, pp. 11282, doi:10.3390/app122111282, 20222076-341710.3390/app122111282https://www.mdpi.com/2076-3417/12/21/11282info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:07:50Zoai:repositorium.sdum.uminho.pt:1822/80564Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:58:54.253392Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Skin byproducts of reinhardtius hippoglossoides (Greenland Halibut) as ecosustainable source of marine collagen |
title |
Skin byproducts of reinhardtius hippoglossoides (Greenland Halibut) as ecosustainable source of marine collagen |
spellingShingle |
Skin byproducts of reinhardtius hippoglossoides (Greenland Halibut) as ecosustainable source of marine collagen Martins, Eva Marine collagens Fish skins Byproduct valorization Active ingredient Circular economy Science & Technology |
title_short |
Skin byproducts of reinhardtius hippoglossoides (Greenland Halibut) as ecosustainable source of marine collagen |
title_full |
Skin byproducts of reinhardtius hippoglossoides (Greenland Halibut) as ecosustainable source of marine collagen |
title_fullStr |
Skin byproducts of reinhardtius hippoglossoides (Greenland Halibut) as ecosustainable source of marine collagen |
title_full_unstemmed |
Skin byproducts of reinhardtius hippoglossoides (Greenland Halibut) as ecosustainable source of marine collagen |
title_sort |
Skin byproducts of reinhardtius hippoglossoides (Greenland Halibut) as ecosustainable source of marine collagen |
author |
Martins, Eva |
author_facet |
Martins, Eva Fernandes, Rita Alves, Ana L. Sousa, Rita O. Reis, R. L. Silva, Tiago H. |
author_role |
author |
author2 |
Fernandes, Rita Alves, Ana L. Sousa, Rita O. Reis, R. L. Silva, Tiago H. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Martins, Eva Fernandes, Rita Alves, Ana L. Sousa, Rita O. Reis, R. L. Silva, Tiago H. |
dc.subject.por.fl_str_mv |
Marine collagens Fish skins Byproduct valorization Active ingredient Circular economy Science & Technology |
topic |
Marine collagens Fish skins Byproduct valorization Active ingredient Circular economy Science & Technology |
description |
Collagen is a ubiquitous protein present in the extracellular matrix of all major metazoan animals, with approximately 28 different human collagen types described in the literature, each with unique physicochemical properties. Collagens found broad application in the cosmeceutical, pharmaceutical, and biomedical fields and can be isolated from environmentally sustainable sources such as marine byproducts, which are abundant in the fish processing industry and are highly appealing low-cost sources. In this study, marine collagen was isolated from the skins of Greenland halibut (Reinhardtius hippoglossoides), an unexplored byproduct from fish processing plants, using three different collagen extraction methods, due to the use of distinct salting-out methods using a solution of 2.6 M NaCl + 0.05 M Tris-HCl pH = 7.5, (method I); a combination of 0.7 M NaCl followed by a solution of 2.3 M NaCl + 0.05 M Tris-HCl pH = 7.5 (method II); and one method using only 0.9 M NaCl (method III), yielding COLRp_I, COLRp_II, and COLRp_III collagens. These extracted type I collagens were produced with a yield of around 2 and 4% and characterized regarding the physicochemical properties, considering possible biotechnological applications. This work evidenced that the typical triple helix structure conformation was preserved in all extraction methods, but influenced the thermal behavior, intrinsic morphology, and moisture capacity of the collagens, with interest for biotechnological application, as the incorporation as an ingredient in cosmetic formulation. Furthermore, the use of collagen isolated from skin byproducts represents a high economic value with decreasing collagen cost for industrial purposes and is also an environmentally sustainable source for industrial uses. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-11 2022-11-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/80564 |
url |
https://hdl.handle.net/1822/80564 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Martins E., Fernandes R., Alves A. L., Sousa R. O., Reis R. L., Silva T. H. Skin byproducts of reinhardtius hippoglossoides (Greenland Halibut) as ecosustainable source of marine collagen, Applied Sciences, Vol. 12, pp. 11282, doi:10.3390/app122111282, 2022 2076-3417 10.3390/app122111282 https://www.mdpi.com/2076-3417/12/21/11282 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799132380365062144 |