Photocrosslinkable Kappa-carrageenan hydrogels for tissue engineering applications

Detalhes bibliográficos
Autor(a) principal: Mihaila, Silvia M.
Data de Publicação: 2013
Outros Autores: Gaharwar, Akhilesh K., Reis, R. L., Marques, A. P., Gomes, Manuela E., Khademhosseini, Ali
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/23345
Resumo: Published online
id RCAP_520593b3b6494f0067dd8c2387973c4d
oai_identifier_str oai:repositorium.sdum.uminho.pt:1822/23345
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Photocrosslinkable Kappa-carrageenan hydrogels for tissue engineering applicationsHydrogelsPatterningPhotocrosslinkable Kappa-CarrageenanTissue engineeringkappa-carrageenanmechanical propertiespolymeric materialsScience & TechnologyPublished onlineKappa carrageenan ( κ -CA) is a natural-origin polymer that closely mimics the glycosaminoglycan structure, one of the most important constituents of native tissues extracellular matrix. Previously, it has been shown that κ -CA can crosslink via ionic interactions rendering strong, but brittle hydrogels. In this study, we introduce photocrosslinkable methacrylate moieties on the κ -CA backbone to create physically and chemically crosslinked hydrogels highlighting their use in the context of tissue engineering. By varying the degree of methacrylation, the effect on hydrogel crosslinking was investigated in terms of hydration degree, dissolution profi les, morphological, mechanical, and rheological properties. Furthermore, the viability of fi broblast cells cultured inside the photocrosslinked hydrogels was investigated. The combination of chemical and physical crosslinking procedures enables the formation of hydrogels with highly versatile physical and chemical properties, while maintaining the viability of encapsulated cells. To our best knowledge, this is the fi rst study reporting the synthesis of photocrosslinkable κ -CA with controllable compressive moduli, swelling ratios and pore size distributions. Moreover, by micromolding approaches, spatially controlled geometries and cell distribution patterns could be obtained, thus enabling the development of cell-material platforms that can be applied and tailored to a broad range of tissue engineering strategies.Authors thank Dr. Alpesh Patel, Dr. Shilpa Sant and Hyeongho Shin for valuable scientific discussions. SMM thanks the Portuguese Foundation for Science and Technology (FCT) for the personal grant SFRH/BD/42968/2008 (MIT-Portugal Program) and the financial support of MIT/ECE/0047/2009 project. AKG would like to thank Prof. Robert Langer (MIT) and MIT-Portugal Program for the financial support. Author Contributions : S. M. M., A. G., and A. K. designed the study; S. M. M. synthesized the MA-kappa-CA, produced the hydrogels, and performed the cells studies, swelling and degradation studies. S.M.M. and A.G. performed the rest of the experimental part; S.M.M., A.G. and A.K. wrote the paper. A.P.M., R.L.R., and M.E.G. revised the paper. All authors discussed the results and commented on the manuscript.WileyUniversidade do MinhoMihaila, Silvia M.Gaharwar, Akhilesh K.Reis, R. L.Marques, A. P.Gomes, Manuela E.Khademhosseini, Ali20132013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/23345eng2192-264010.1002/adhm.20120031723281344http://onlinelibrary.wiley.com/doi/10.1002/adhm.201200317/abstractinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T11:55:29Zoai:repositorium.sdum.uminho.pt:1822/23345Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:45:02.629987Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Photocrosslinkable Kappa-carrageenan hydrogels for tissue engineering applications
title Photocrosslinkable Kappa-carrageenan hydrogels for tissue engineering applications
spellingShingle Photocrosslinkable Kappa-carrageenan hydrogels for tissue engineering applications
Mihaila, Silvia M.
Hydrogels
Patterning
Photocrosslinkable Kappa-Carrageenan
Tissue engineering
kappa-carrageenan
mechanical properties
polymeric materials
Science & Technology
title_short Photocrosslinkable Kappa-carrageenan hydrogels for tissue engineering applications
title_full Photocrosslinkable Kappa-carrageenan hydrogels for tissue engineering applications
title_fullStr Photocrosslinkable Kappa-carrageenan hydrogels for tissue engineering applications
title_full_unstemmed Photocrosslinkable Kappa-carrageenan hydrogels for tissue engineering applications
title_sort Photocrosslinkable Kappa-carrageenan hydrogels for tissue engineering applications
author Mihaila, Silvia M.
author_facet Mihaila, Silvia M.
Gaharwar, Akhilesh K.
Reis, R. L.
Marques, A. P.
Gomes, Manuela E.
Khademhosseini, Ali
author_role author
author2 Gaharwar, Akhilesh K.
Reis, R. L.
Marques, A. P.
Gomes, Manuela E.
Khademhosseini, Ali
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Mihaila, Silvia M.
Gaharwar, Akhilesh K.
Reis, R. L.
Marques, A. P.
Gomes, Manuela E.
Khademhosseini, Ali
dc.subject.por.fl_str_mv Hydrogels
Patterning
Photocrosslinkable Kappa-Carrageenan
Tissue engineering
kappa-carrageenan
mechanical properties
polymeric materials
Science & Technology
topic Hydrogels
Patterning
Photocrosslinkable Kappa-Carrageenan
Tissue engineering
kappa-carrageenan
mechanical properties
polymeric materials
Science & Technology
description Published online
publishDate 2013
dc.date.none.fl_str_mv 2013
2013-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/23345
url http://hdl.handle.net/1822/23345
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2192-2640
10.1002/adhm.201200317
23281344
http://onlinelibrary.wiley.com/doi/10.1002/adhm.201200317/abstract
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799132202163765248

Registros relacionados