The role of strong hypoxia in tumors after treatment in the outcome of bacteriochlorin-based photodynamic therapy
Autor(a) principal: | |
---|---|
Data de Publicação: | 2014 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/27908 https://doi.org/10.1016/j.freeradbiomed.2014.05.003 |
Resumo: | Blood flow and pO2 changes after vascular-targeted photodynamic therapy (V-PDT) or cellular-targeted PDT (C-PDT) using 5,10,15,20-tetrakis(2,6-difluoro-3-N-methylsulfamoylphenyl) bacteriochlorin (F2BMet) as photosensitizer were investigated in DBA/2 mice with S91 Cloudman mouse melanoma, and correlated with long-term tumor responses. F2BMet generates both singlet oxygen and hydroxyl radicals under near-infrared radiation, which consume oxygen. Partial oxygen pressure was lowered in PDT-treated tumors and this was ascribed both to oxygen consumption during PDT and to fluctuations in oxygen transport after PDT. Similarly, microcirculatory blood flow changed as a result of the disruption of blood vessels by the treatment. A novel noninvasive approach combining electron paramagnetic resonance oximetry and laser Doppler blood perfusion measurements allowed longitudinal monitoring of hypoxia and vascular function changes in the same animals, after PDT. C-PDT induced parallel changes in tumor pO2 and blood flow, i.e., an initial decrease immediately after treatment, followed by a slow increase. In contrast, V-PDT led to a strong and persistent depletion of pO2, although the microcirculatory blood flow increased. Strong hypoxia after V-PDT led to a slight increase in VEGF level 24 h after treatment. C-PDT caused a ca. 5-day delay in tumor growth, whereas V-PDT was much more efficient and led to tumor growth inhibition in 90% of animals. The tumors of 44% of mice treated with V-PDT regressed completely and did not reappear for over 1 year. In conclusion, mild and transient hypoxia after C-PDT led to intense pO2 compensatory effects and modest tumor inhibition, but strong and persistent local hypoxia after V-PDT caused tumor growth inhibition. |
id |
RCAP_52508bb680cc28f86dc668bb99d073b9 |
---|---|
oai_identifier_str |
oai:estudogeral.uc.pt:10316/27908 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
The role of strong hypoxia in tumors after treatment in the outcome of bacteriochlorin-based photodynamic therapyOxymetryBlood flowVascular-targeted PDTBacteriochlorinsHydroxyl radicalSinglet oxygenSuperoxidePhototoxicityPhotodynamic therapyFree radicalsBlood flow and pO2 changes after vascular-targeted photodynamic therapy (V-PDT) or cellular-targeted PDT (C-PDT) using 5,10,15,20-tetrakis(2,6-difluoro-3-N-methylsulfamoylphenyl) bacteriochlorin (F2BMet) as photosensitizer were investigated in DBA/2 mice with S91 Cloudman mouse melanoma, and correlated with long-term tumor responses. F2BMet generates both singlet oxygen and hydroxyl radicals under near-infrared radiation, which consume oxygen. Partial oxygen pressure was lowered in PDT-treated tumors and this was ascribed both to oxygen consumption during PDT and to fluctuations in oxygen transport after PDT. Similarly, microcirculatory blood flow changed as a result of the disruption of blood vessels by the treatment. A novel noninvasive approach combining electron paramagnetic resonance oximetry and laser Doppler blood perfusion measurements allowed longitudinal monitoring of hypoxia and vascular function changes in the same animals, after PDT. C-PDT induced parallel changes in tumor pO2 and blood flow, i.e., an initial decrease immediately after treatment, followed by a slow increase. In contrast, V-PDT led to a strong and persistent depletion of pO2, although the microcirculatory blood flow increased. Strong hypoxia after V-PDT led to a slight increase in VEGF level 24 h after treatment. C-PDT caused a ca. 5-day delay in tumor growth, whereas V-PDT was much more efficient and led to tumor growth inhibition in 90% of animals. The tumors of 44% of mice treated with V-PDT regressed completely and did not reappear for over 1 year. In conclusion, mild and transient hypoxia after C-PDT led to intense pO2 compensatory effects and modest tumor inhibition, but strong and persistent local hypoxia after V-PDT caused tumor growth inhibition.Elsevier2014-08info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/27908http://hdl.handle.net/10316/27908https://doi.org/10.1016/j.freeradbiomed.2014.05.003engKRZYKAWSKA-SERDA, Martyna [et. al] - The role of strong hypoxia in tumors after treatment in the outcome of bacteriochlorin-based photodynamic therapy. "Free Radical Biology and Medicine". ISSN 0891-5849. Vol. 73 (2014) p. 239–2510891-5849http://www.sciencedirect.com/science/article/pii/S089158491400210XKrzykawska-Serda, MartynaDąbrowski, Janusz M.Arnaut, Luis G.Szczygieł, MałgorzataUrbańska, KrystynaStochel, GrażynaElas, Martynainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2020-05-25T13:18:19Zoai:estudogeral.uc.pt:10316/27908Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:01:50.752921Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
The role of strong hypoxia in tumors after treatment in the outcome of bacteriochlorin-based photodynamic therapy |
title |
The role of strong hypoxia in tumors after treatment in the outcome of bacteriochlorin-based photodynamic therapy |
spellingShingle |
The role of strong hypoxia in tumors after treatment in the outcome of bacteriochlorin-based photodynamic therapy Krzykawska-Serda, Martyna Oxymetry Blood flow Vascular-targeted PDT Bacteriochlorins Hydroxyl radical Singlet oxygen Superoxide Phototoxicity Photodynamic therapy Free radicals |
title_short |
The role of strong hypoxia in tumors after treatment in the outcome of bacteriochlorin-based photodynamic therapy |
title_full |
The role of strong hypoxia in tumors after treatment in the outcome of bacteriochlorin-based photodynamic therapy |
title_fullStr |
The role of strong hypoxia in tumors after treatment in the outcome of bacteriochlorin-based photodynamic therapy |
title_full_unstemmed |
The role of strong hypoxia in tumors after treatment in the outcome of bacteriochlorin-based photodynamic therapy |
title_sort |
The role of strong hypoxia in tumors after treatment in the outcome of bacteriochlorin-based photodynamic therapy |
author |
Krzykawska-Serda, Martyna |
author_facet |
Krzykawska-Serda, Martyna Dąbrowski, Janusz M. Arnaut, Luis G. Szczygieł, Małgorzata Urbańska, Krystyna Stochel, Grażyna Elas, Martyna |
author_role |
author |
author2 |
Dąbrowski, Janusz M. Arnaut, Luis G. Szczygieł, Małgorzata Urbańska, Krystyna Stochel, Grażyna Elas, Martyna |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Krzykawska-Serda, Martyna Dąbrowski, Janusz M. Arnaut, Luis G. Szczygieł, Małgorzata Urbańska, Krystyna Stochel, Grażyna Elas, Martyna |
dc.subject.por.fl_str_mv |
Oxymetry Blood flow Vascular-targeted PDT Bacteriochlorins Hydroxyl radical Singlet oxygen Superoxide Phototoxicity Photodynamic therapy Free radicals |
topic |
Oxymetry Blood flow Vascular-targeted PDT Bacteriochlorins Hydroxyl radical Singlet oxygen Superoxide Phototoxicity Photodynamic therapy Free radicals |
description |
Blood flow and pO2 changes after vascular-targeted photodynamic therapy (V-PDT) or cellular-targeted PDT (C-PDT) using 5,10,15,20-tetrakis(2,6-difluoro-3-N-methylsulfamoylphenyl) bacteriochlorin (F2BMet) as photosensitizer were investigated in DBA/2 mice with S91 Cloudman mouse melanoma, and correlated with long-term tumor responses. F2BMet generates both singlet oxygen and hydroxyl radicals under near-infrared radiation, which consume oxygen. Partial oxygen pressure was lowered in PDT-treated tumors and this was ascribed both to oxygen consumption during PDT and to fluctuations in oxygen transport after PDT. Similarly, microcirculatory blood flow changed as a result of the disruption of blood vessels by the treatment. A novel noninvasive approach combining electron paramagnetic resonance oximetry and laser Doppler blood perfusion measurements allowed longitudinal monitoring of hypoxia and vascular function changes in the same animals, after PDT. C-PDT induced parallel changes in tumor pO2 and blood flow, i.e., an initial decrease immediately after treatment, followed by a slow increase. In contrast, V-PDT led to a strong and persistent depletion of pO2, although the microcirculatory blood flow increased. Strong hypoxia after V-PDT led to a slight increase in VEGF level 24 h after treatment. C-PDT caused a ca. 5-day delay in tumor growth, whereas V-PDT was much more efficient and led to tumor growth inhibition in 90% of animals. The tumors of 44% of mice treated with V-PDT regressed completely and did not reappear for over 1 year. In conclusion, mild and transient hypoxia after C-PDT led to intense pO2 compensatory effects and modest tumor inhibition, but strong and persistent local hypoxia after V-PDT caused tumor growth inhibition. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-08 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/27908 http://hdl.handle.net/10316/27908 https://doi.org/10.1016/j.freeradbiomed.2014.05.003 |
url |
http://hdl.handle.net/10316/27908 https://doi.org/10.1016/j.freeradbiomed.2014.05.003 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
KRZYKAWSKA-SERDA, Martyna [et. al] - The role of strong hypoxia in tumors after treatment in the outcome of bacteriochlorin-based photodynamic therapy. "Free Radical Biology and Medicine". ISSN 0891-5849. Vol. 73 (2014) p. 239–251 0891-5849 http://www.sciencedirect.com/science/article/pii/S089158491400210X |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799133908850180096 |