Effect of condensed tannin ingestion in sheep and goat parotid saliva proteome
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10174/3888 |
Resumo: | Saliva appears as a defence mechanism, against potential negative effects of tannins, in some species of animals which have to deal with these plant secondary metabolites in their regular diets. This study was carried out to investigate changes in parotid saliva protein profiles of sheep Ovis aries)and goats (Capra hircus), induced by condensed tannin ingestion. Five Merino sheep and five Serpentina goats were maintained on a quebracho tannin enriched diet for 10 days. Saliva was collected through catheters inserted on parotid ducts and salivary proteins were separated by two dimensional gel electrophoresis. Matrix assisted Laser desorption ionization – time of flight(MALDI-TOF)and liquid chromatography tandem mass spectrometry (LC-MS/MS) were used to identify the proteins whose expression levels changed after tannin consumption. Although no new proteins appeared, quebracho tannin consumption increased saliva total protein concentration and produced changes in the proteome of both species. While some proteins were similarly altered in both species parotid salivary protein profile, sheep and goats also presented species-specific differences in response to tannin consumption. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Effect of condensed tannin ingestion in sheep and goat parotid saliva proteomecondensed tanningoats (Capra hircus)quebrachoparotid saliva proteomesheep (Ovis aries)Saliva appears as a defence mechanism, against potential negative effects of tannins, in some species of animals which have to deal with these plant secondary metabolites in their regular diets. This study was carried out to investigate changes in parotid saliva protein profiles of sheep Ovis aries)and goats (Capra hircus), induced by condensed tannin ingestion. Five Merino sheep and five Serpentina goats were maintained on a quebracho tannin enriched diet for 10 days. Saliva was collected through catheters inserted on parotid ducts and salivary proteins were separated by two dimensional gel electrophoresis. Matrix assisted Laser desorption ionization – time of flight(MALDI-TOF)and liquid chromatography tandem mass spectrometry (LC-MS/MS) were used to identify the proteins whose expression levels changed after tannin consumption. Although no new proteins appeared, quebracho tannin consumption increased saliva total protein concentration and produced changes in the proteome of both species. While some proteins were similarly altered in both species parotid salivary protein profile, sheep and goats also presented species-specific differences in response to tannin consumption.Wiley2012-01-19T16:22:30Z2012-01-192011-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10174/3888http://hdl.handle.net/10174/3888porLamy E, da Costa G, Santos R, Capela e Silva F, Potes J, Pereira A, Coelho AV, Sales Baptista E. Effect of condensed tannin ingestion in sheep and goat parotid saliva proteome. Journal of Animal Physiology and Animal Nutrition 2011; 95: 304-312.304-312http://onlinelibrary.wiley.com/doi/10.1111/j.1439-0396.2010.01055.x/abstract95Journal of Animal Physiology and Animal NutritionBIOecsl@uevora.ptndndfcs@uevora.ptjacpotes@uevora.ptapereira@uevora.ptvarela@itqb.unl.ptelsaba@uevora.pt379Lamy, Eda Costa, GSantos, RCapela e Silva, FPotes, JPereira, ACoelho, AVSales Baptista, Einfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T18:41:23Zoai:dspace.uevora.pt:10174/3888Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:59:15.219104Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Effect of condensed tannin ingestion in sheep and goat parotid saliva proteome |
title |
Effect of condensed tannin ingestion in sheep and goat parotid saliva proteome |
spellingShingle |
Effect of condensed tannin ingestion in sheep and goat parotid saliva proteome Lamy, E condensed tannin goats (Capra hircus) quebracho parotid saliva proteome sheep (Ovis aries) |
title_short |
Effect of condensed tannin ingestion in sheep and goat parotid saliva proteome |
title_full |
Effect of condensed tannin ingestion in sheep and goat parotid saliva proteome |
title_fullStr |
Effect of condensed tannin ingestion in sheep and goat parotid saliva proteome |
title_full_unstemmed |
Effect of condensed tannin ingestion in sheep and goat parotid saliva proteome |
title_sort |
Effect of condensed tannin ingestion in sheep and goat parotid saliva proteome |
author |
Lamy, E |
author_facet |
Lamy, E da Costa, G Santos, R Capela e Silva, F Potes, J Pereira, A Coelho, AV Sales Baptista, E |
author_role |
author |
author2 |
da Costa, G Santos, R Capela e Silva, F Potes, J Pereira, A Coelho, AV Sales Baptista, E |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Lamy, E da Costa, G Santos, R Capela e Silva, F Potes, J Pereira, A Coelho, AV Sales Baptista, E |
dc.subject.por.fl_str_mv |
condensed tannin goats (Capra hircus) quebracho parotid saliva proteome sheep (Ovis aries) |
topic |
condensed tannin goats (Capra hircus) quebracho parotid saliva proteome sheep (Ovis aries) |
description |
Saliva appears as a defence mechanism, against potential negative effects of tannins, in some species of animals which have to deal with these plant secondary metabolites in their regular diets. This study was carried out to investigate changes in parotid saliva protein profiles of sheep Ovis aries)and goats (Capra hircus), induced by condensed tannin ingestion. Five Merino sheep and five Serpentina goats were maintained on a quebracho tannin enriched diet for 10 days. Saliva was collected through catheters inserted on parotid ducts and salivary proteins were separated by two dimensional gel electrophoresis. Matrix assisted Laser desorption ionization – time of flight(MALDI-TOF)and liquid chromatography tandem mass spectrometry (LC-MS/MS) were used to identify the proteins whose expression levels changed after tannin consumption. Although no new proteins appeared, quebracho tannin consumption increased saliva total protein concentration and produced changes in the proteome of both species. While some proteins were similarly altered in both species parotid salivary protein profile, sheep and goats also presented species-specific differences in response to tannin consumption. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-01-01T00:00:00Z 2012-01-19T16:22:30Z 2012-01-19 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10174/3888 http://hdl.handle.net/10174/3888 |
url |
http://hdl.handle.net/10174/3888 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Lamy E, da Costa G, Santos R, Capela e Silva F, Potes J, Pereira A, Coelho AV, Sales Baptista E. Effect of condensed tannin ingestion in sheep and goat parotid saliva proteome. Journal of Animal Physiology and Animal Nutrition 2011; 95: 304-312. 304-312 http://onlinelibrary.wiley.com/doi/10.1111/j.1439-0396.2010.01055.x/abstract 95 Journal of Animal Physiology and Animal Nutrition BIO ecsl@uevora.pt nd nd fcs@uevora.pt jacpotes@uevora.pt apereira@uevora.pt varela@itqb.unl.pt elsaba@uevora.pt 379 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Wiley |
publisher.none.fl_str_mv |
Wiley |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799136475304951808 |