Vancomycin-Loaded, Nanohydroxyapatite-Based Scaffold for Osteomyelitis Treatment: In Vivo Rabbit Toxicological Tests and In Vivo Efficacy Tests in a Sheep Model
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10174/34656 https://doi.org/Alegrete, N., Sousa, S. R., Padrão, T., Carvalho, Â., Lucas, R., Canadas, R. F., Lavrador, C., Alexandre, N., Gärtner, F., Monteiro, F. J., & Gutierres, M. (2023). Vancomycin-Loaded, Nanohydroxyapatite-Based Scaffold for Osteomyelitis Treatment: In Vivo Rabbit Toxicological Tests and In Vivo Efficacy Tests in a Sheep Model. Bioengineering, 10(2), 206. https://doi.org/10.3390/bioengineering10020206 https://doi.org/10.3390/bioengineering10020206 |
Resumo: | The treatment for osteomyelitis consists of surgical debridement, filling of the dead space, soft tissue coverage, and intravenous administration of antimicrobial (AM) agents for long periods. Biomaterials for local delivery of AM agents, while providing controllable antibiotic release rates and simultaneously acting as a bone scaffold, may be a valuable alternative; thus, avoiding systemic AM side effects. V-HEPHAPC is a heparinized nanohydroxyapatite (nHA)/collagen biocomposite loaded with vancomycin that has been previously studied and tested in vitro. It enables a vancomycin-releasing profile with an intense initial burst, followed by a sustained release with concentrations above the Minimum Inhibitory Concentration (MIC) for MRSA. In vitro results have also shown that cellular viability is not compromised, suggesting that V-HEPHAPC granules may be a promising alternative device for the treatment of osteomyelitis. In the present study, V-HEPHAPC (HEPHAPC with vancomycin) granules were used as a vancomycin carrier to treat MRSA osteomyelitis. First, in vivo Good Laboratory Practice (GLP) toxicological tests were performed in a rabbit model, assuring that HEPHAPC and V-HEPHAPC have no relevant side effects. Second, V-HEPHAPC proved to be an efficient drug carrier and bone substitute to control MRSA infection and simultaneously reconstruct the bone cavity in a sheep model. |
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Vancomycin-Loaded, Nanohydroxyapatite-Based Scaffold for Osteomyelitis Treatment: In Vivo Rabbit Toxicological Tests and In Vivo Efficacy Tests in a Sheep Modeldrug deliverynanohydroxyapatiteosteomyelitisvancomycinThe treatment for osteomyelitis consists of surgical debridement, filling of the dead space, soft tissue coverage, and intravenous administration of antimicrobial (AM) agents for long periods. Biomaterials for local delivery of AM agents, while providing controllable antibiotic release rates and simultaneously acting as a bone scaffold, may be a valuable alternative; thus, avoiding systemic AM side effects. V-HEPHAPC is a heparinized nanohydroxyapatite (nHA)/collagen biocomposite loaded with vancomycin that has been previously studied and tested in vitro. It enables a vancomycin-releasing profile with an intense initial burst, followed by a sustained release with concentrations above the Minimum Inhibitory Concentration (MIC) for MRSA. In vitro results have also shown that cellular viability is not compromised, suggesting that V-HEPHAPC granules may be a promising alternative device for the treatment of osteomyelitis. In the present study, V-HEPHAPC (HEPHAPC with vancomycin) granules were used as a vancomycin carrier to treat MRSA osteomyelitis. First, in vivo Good Laboratory Practice (GLP) toxicological tests were performed in a rabbit model, assuring that HEPHAPC and V-HEPHAPC have no relevant side effects. Second, V-HEPHAPC proved to be an efficient drug carrier and bone substitute to control MRSA infection and simultaneously reconstruct the bone cavity in a sheep model.MDPI2023-02-24T10:34:52Z2023-02-242023-02-04T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10174/34656https://doi.org/Alegrete, N., Sousa, S. R., Padrão, T., Carvalho, Â., Lucas, R., Canadas, R. F., Lavrador, C., Alexandre, N., Gärtner, F., Monteiro, F. J., & Gutierres, M. (2023). Vancomycin-Loaded, Nanohydroxyapatite-Based Scaffold for Osteomyelitis Treatment: In Vivo Rabbit Toxicological Tests and In Vivo Efficacy Tests in a Sheep Model. Bioengineering, 10(2), 206. https://doi.org/10.3390/bioengineering10020206http://hdl.handle.net/10174/34656https://doi.org/10.3390/bioengineering10020206eng1-192306-5354https://www.mdpi.com/2306-5354/10/2/20610Bioengineering206ndndndndndndclavrador@uevora.ptnmla@uevora.ptndndndNext Generation Bioengineered Strategies for Musculoskeletal Regeneration376Alegrete, NunoSousa, Susana R.Padrão, TatianaCarvalho, ÂngelaLucas, RaquelCanadas, Raphael F.Lavrador, CatarinaAlexandre, NunoGärtner, FátimaMonteiro, Fernando J.Gutierres, Manuelinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-08-08T04:51:24ZPortal AgregadorONG |
dc.title.none.fl_str_mv |
Vancomycin-Loaded, Nanohydroxyapatite-Based Scaffold for Osteomyelitis Treatment: In Vivo Rabbit Toxicological Tests and In Vivo Efficacy Tests in a Sheep Model |
title |
Vancomycin-Loaded, Nanohydroxyapatite-Based Scaffold for Osteomyelitis Treatment: In Vivo Rabbit Toxicological Tests and In Vivo Efficacy Tests in a Sheep Model |
spellingShingle |
Vancomycin-Loaded, Nanohydroxyapatite-Based Scaffold for Osteomyelitis Treatment: In Vivo Rabbit Toxicological Tests and In Vivo Efficacy Tests in a Sheep Model Alegrete, Nuno drug delivery nanohydroxyapatite osteomyelitis vancomycin |
title_short |
Vancomycin-Loaded, Nanohydroxyapatite-Based Scaffold for Osteomyelitis Treatment: In Vivo Rabbit Toxicological Tests and In Vivo Efficacy Tests in a Sheep Model |
title_full |
Vancomycin-Loaded, Nanohydroxyapatite-Based Scaffold for Osteomyelitis Treatment: In Vivo Rabbit Toxicological Tests and In Vivo Efficacy Tests in a Sheep Model |
title_fullStr |
Vancomycin-Loaded, Nanohydroxyapatite-Based Scaffold for Osteomyelitis Treatment: In Vivo Rabbit Toxicological Tests and In Vivo Efficacy Tests in a Sheep Model |
title_full_unstemmed |
Vancomycin-Loaded, Nanohydroxyapatite-Based Scaffold for Osteomyelitis Treatment: In Vivo Rabbit Toxicological Tests and In Vivo Efficacy Tests in a Sheep Model |
title_sort |
Vancomycin-Loaded, Nanohydroxyapatite-Based Scaffold for Osteomyelitis Treatment: In Vivo Rabbit Toxicological Tests and In Vivo Efficacy Tests in a Sheep Model |
author |
Alegrete, Nuno |
author_facet |
Alegrete, Nuno Sousa, Susana R. Padrão, Tatiana Carvalho, Ângela Lucas, Raquel Canadas, Raphael F. Lavrador, Catarina Alexandre, Nuno Gärtner, Fátima Monteiro, Fernando J. Gutierres, Manuel |
author_role |
author |
author2 |
Sousa, Susana R. Padrão, Tatiana Carvalho, Ângela Lucas, Raquel Canadas, Raphael F. Lavrador, Catarina Alexandre, Nuno Gärtner, Fátima Monteiro, Fernando J. Gutierres, Manuel |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Alegrete, Nuno Sousa, Susana R. Padrão, Tatiana Carvalho, Ângela Lucas, Raquel Canadas, Raphael F. Lavrador, Catarina Alexandre, Nuno Gärtner, Fátima Monteiro, Fernando J. Gutierres, Manuel |
dc.subject.por.fl_str_mv |
drug delivery nanohydroxyapatite osteomyelitis vancomycin |
topic |
drug delivery nanohydroxyapatite osteomyelitis vancomycin |
description |
The treatment for osteomyelitis consists of surgical debridement, filling of the dead space, soft tissue coverage, and intravenous administration of antimicrobial (AM) agents for long periods. Biomaterials for local delivery of AM agents, while providing controllable antibiotic release rates and simultaneously acting as a bone scaffold, may be a valuable alternative; thus, avoiding systemic AM side effects. V-HEPHAPC is a heparinized nanohydroxyapatite (nHA)/collagen biocomposite loaded with vancomycin that has been previously studied and tested in vitro. It enables a vancomycin-releasing profile with an intense initial burst, followed by a sustained release with concentrations above the Minimum Inhibitory Concentration (MIC) for MRSA. In vitro results have also shown that cellular viability is not compromised, suggesting that V-HEPHAPC granules may be a promising alternative device for the treatment of osteomyelitis. In the present study, V-HEPHAPC (HEPHAPC with vancomycin) granules were used as a vancomycin carrier to treat MRSA osteomyelitis. First, in vivo Good Laboratory Practice (GLP) toxicological tests were performed in a rabbit model, assuring that HEPHAPC and V-HEPHAPC have no relevant side effects. Second, V-HEPHAPC proved to be an efficient drug carrier and bone substitute to control MRSA infection and simultaneously reconstruct the bone cavity in a sheep model. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-02-24T10:34:52Z 2023-02-24 2023-02-04T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10174/34656 https://doi.org/Alegrete, N., Sousa, S. R., Padrão, T., Carvalho, Â., Lucas, R., Canadas, R. F., Lavrador, C., Alexandre, N., Gärtner, F., Monteiro, F. J., & Gutierres, M. (2023). Vancomycin-Loaded, Nanohydroxyapatite-Based Scaffold for Osteomyelitis Treatment: In Vivo Rabbit Toxicological Tests and In Vivo Efficacy Tests in a Sheep Model. Bioengineering, 10(2), 206. https://doi.org/10.3390/bioengineering10020206 http://hdl.handle.net/10174/34656 https://doi.org/10.3390/bioengineering10020206 |
url |
http://hdl.handle.net/10174/34656 https://doi.org/Alegrete, N., Sousa, S. R., Padrão, T., Carvalho, Â., Lucas, R., Canadas, R. F., Lavrador, C., Alexandre, N., Gärtner, F., Monteiro, F. J., & Gutierres, M. (2023). Vancomycin-Loaded, Nanohydroxyapatite-Based Scaffold for Osteomyelitis Treatment: In Vivo Rabbit Toxicological Tests and In Vivo Efficacy Tests in a Sheep Model. Bioengineering, 10(2), 206. https://doi.org/10.3390/bioengineering10020206 https://doi.org/10.3390/bioengineering10020206 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1-19 2306-5354 https://www.mdpi.com/2306-5354/10/2/206 10 Bioengineering 206 nd nd nd nd nd nd clavrador@uevora.pt nmla@uevora.pt nd nd nd Next Generation Bioengineered Strategies for Musculoskeletal Regeneration 376 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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