GuttaFlow® Bioseal Cytotoxicity Assessment: In Vitro Study
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/106487 https://doi.org/10.3390/molecules25184297 |
Resumo: | The sealers used for root canal treatment should be biocompatible for the peri-radicular tissues, to evaluate the cytotoxic effects of GuttaFlow® bioseal sealer and to compare them with AH26® epoxy resin. Culture media were conditioned with the GuttaFlow® bioseal and AH26® pellets. MDPC-23 odontoblast cell cultures were treated with conditioned medium and serial dilutions. To evaluate the metabolic activity and cellular viability, the MTT and SRB assays were performed. To determine the production of reactive oxygen species, the DHE and DCF-DA probes were used. Cell cycle and cell-death types were assessed by cytometry, and to evaluate the mineralization capacity, the Alizarin Red S coloration was used. Statistical analysis was performed using analysis of variance (ANOVA) when normality was found and Kruskal-Wallis on the opposite case. For the comparison with normality values, the Student t-test was used. Cells exposed to the GuttaFlow® bioseal conditioned medium maintained high metabolic activities, except at higher concentrations. Likewise, viability was maintained, but a significant decrease was observed after exposure to the highest concentration (p < 0.001), associated with cell death by late apoptosis and necrosis. When cell cultures were exposed to AH26®, metabolic activity was highly compromised, resulting in cell death. An imbalance in the production of peroxides and superoxide anion was observed. GuttaFlow® bioseal showed higher biocompatibility than AH26®. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
spelling |
GuttaFlow® Bioseal Cytotoxicity Assessment: In Vitro Studyepoxy resinstricalciumphosphatecytotoxicitycell viabilityGuttaFlow® biosealAH26®Biocompatible MaterialsCell Cycle CheckpointsCell LineCell SurvivalDimethylpolysiloxanesDrug CombinationsGutta-PerchaHumansOxidative StressRoot Canal Filling MaterialsThe sealers used for root canal treatment should be biocompatible for the peri-radicular tissues, to evaluate the cytotoxic effects of GuttaFlow® bioseal sealer and to compare them with AH26® epoxy resin. Culture media were conditioned with the GuttaFlow® bioseal and AH26® pellets. MDPC-23 odontoblast cell cultures were treated with conditioned medium and serial dilutions. To evaluate the metabolic activity and cellular viability, the MTT and SRB assays were performed. To determine the production of reactive oxygen species, the DHE and DCF-DA probes were used. Cell cycle and cell-death types were assessed by cytometry, and to evaluate the mineralization capacity, the Alizarin Red S coloration was used. Statistical analysis was performed using analysis of variance (ANOVA) when normality was found and Kruskal-Wallis on the opposite case. For the comparison with normality values, the Student t-test was used. Cells exposed to the GuttaFlow® bioseal conditioned medium maintained high metabolic activities, except at higher concentrations. Likewise, viability was maintained, but a significant decrease was observed after exposure to the highest concentration (p < 0.001), associated with cell death by late apoptosis and necrosis. When cell cultures were exposed to AH26®, metabolic activity was highly compromised, resulting in cell death. An imbalance in the production of peroxides and superoxide anion was observed. GuttaFlow® bioseal showed higher biocompatibility than AH26®.MDPI2020-09-19info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/106487http://hdl.handle.net/10316/106487https://doi.org/10.3390/molecules25184297eng1420-3049Ferreira, InêsLaranjo, MafaldaMarto, Carlos MiguelCasalta-Lopes, JoãoSerambeque, BeatrizGonçalves, Ana CristinaSarmento-Ribeiro, Ana BelaCarrilho, EuniceBotelho, Maria FilomenaPaula, Anabela BaptistaFerreira, Manuel Marquesinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-04-05T20:44:13Zoai:estudogeral.uc.pt:10316/106487Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:22:56.182126Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
GuttaFlow® Bioseal Cytotoxicity Assessment: In Vitro Study |
title |
GuttaFlow® Bioseal Cytotoxicity Assessment: In Vitro Study |
spellingShingle |
GuttaFlow® Bioseal Cytotoxicity Assessment: In Vitro Study Ferreira, Inês epoxy resins tricalciumphosphate cytotoxicity cell viability GuttaFlow® bioseal AH26® Biocompatible Materials Cell Cycle Checkpoints Cell Line Cell Survival Dimethylpolysiloxanes Drug Combinations Gutta-Percha Humans Oxidative Stress Root Canal Filling Materials |
title_short |
GuttaFlow® Bioseal Cytotoxicity Assessment: In Vitro Study |
title_full |
GuttaFlow® Bioseal Cytotoxicity Assessment: In Vitro Study |
title_fullStr |
GuttaFlow® Bioseal Cytotoxicity Assessment: In Vitro Study |
title_full_unstemmed |
GuttaFlow® Bioseal Cytotoxicity Assessment: In Vitro Study |
title_sort |
GuttaFlow® Bioseal Cytotoxicity Assessment: In Vitro Study |
author |
Ferreira, Inês |
author_facet |
Ferreira, Inês Laranjo, Mafalda Marto, Carlos Miguel Casalta-Lopes, João Serambeque, Beatriz Gonçalves, Ana Cristina Sarmento-Ribeiro, Ana Bela Carrilho, Eunice Botelho, Maria Filomena Paula, Anabela Baptista Ferreira, Manuel Marques |
author_role |
author |
author2 |
Laranjo, Mafalda Marto, Carlos Miguel Casalta-Lopes, João Serambeque, Beatriz Gonçalves, Ana Cristina Sarmento-Ribeiro, Ana Bela Carrilho, Eunice Botelho, Maria Filomena Paula, Anabela Baptista Ferreira, Manuel Marques |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Ferreira, Inês Laranjo, Mafalda Marto, Carlos Miguel Casalta-Lopes, João Serambeque, Beatriz Gonçalves, Ana Cristina Sarmento-Ribeiro, Ana Bela Carrilho, Eunice Botelho, Maria Filomena Paula, Anabela Baptista Ferreira, Manuel Marques |
dc.subject.por.fl_str_mv |
epoxy resins tricalciumphosphate cytotoxicity cell viability GuttaFlow® bioseal AH26® Biocompatible Materials Cell Cycle Checkpoints Cell Line Cell Survival Dimethylpolysiloxanes Drug Combinations Gutta-Percha Humans Oxidative Stress Root Canal Filling Materials |
topic |
epoxy resins tricalciumphosphate cytotoxicity cell viability GuttaFlow® bioseal AH26® Biocompatible Materials Cell Cycle Checkpoints Cell Line Cell Survival Dimethylpolysiloxanes Drug Combinations Gutta-Percha Humans Oxidative Stress Root Canal Filling Materials |
description |
The sealers used for root canal treatment should be biocompatible for the peri-radicular tissues, to evaluate the cytotoxic effects of GuttaFlow® bioseal sealer and to compare them with AH26® epoxy resin. Culture media were conditioned with the GuttaFlow® bioseal and AH26® pellets. MDPC-23 odontoblast cell cultures were treated with conditioned medium and serial dilutions. To evaluate the metabolic activity and cellular viability, the MTT and SRB assays were performed. To determine the production of reactive oxygen species, the DHE and DCF-DA probes were used. Cell cycle and cell-death types were assessed by cytometry, and to evaluate the mineralization capacity, the Alizarin Red S coloration was used. Statistical analysis was performed using analysis of variance (ANOVA) when normality was found and Kruskal-Wallis on the opposite case. For the comparison with normality values, the Student t-test was used. Cells exposed to the GuttaFlow® bioseal conditioned medium maintained high metabolic activities, except at higher concentrations. Likewise, viability was maintained, but a significant decrease was observed after exposure to the highest concentration (p < 0.001), associated with cell death by late apoptosis and necrosis. When cell cultures were exposed to AH26®, metabolic activity was highly compromised, resulting in cell death. An imbalance in the production of peroxides and superoxide anion was observed. GuttaFlow® bioseal showed higher biocompatibility than AH26®. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-09-19 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/106487 http://hdl.handle.net/10316/106487 https://doi.org/10.3390/molecules25184297 |
url |
http://hdl.handle.net/10316/106487 https://doi.org/10.3390/molecules25184297 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1420-3049 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799134117321768960 |