Novel pectin and nanocellulose based bioinks for 3D bioprinting applications

Detalhes bibliográficos
Autor(a) principal: Guincho, Pedro Batalha
Data de Publicação: 2021
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10773/30763
Resumo: The implementation of personalized and efficient strategies for damaged tissue/organ replacement is becoming increasingly urgent, being observed significant advancements in the field of tissue engineering in recent years, namely in 3D bioprinting of functional living tissue analogues. This automatized technology allows a wide control over the cellular environment and the overall tissue organization. To obtain a fully developed and functional tissue/organ, the bioprinting method and the bioink need to have adequate properties and functionalities, for this reason, a thoughtful choice is crucial. Recently, there have been great developments in the field of bioinks for bioprinting applications. However, the developed bioinks still present several limitations, such as weak mechanical properties, low viability, or high production costs. In this context, the objective of this dissertation is the development of a pectinnanocellulose fibers hydrogel-based bioink laden with human keratinocyte cells (HaCaT cells) for 3D bioprinting. These biopolymers were chosen due to their great potential for 3D bioprinting applications. Pectin is biodegradable, hydrophilic, and can form hydrogels in the presence of divalent cations, such as calcium. Nanocellulose has also already demonstrated its potentialities in 3Dbioprinting, revealing excellent mechanical properties and biocompatibility. However, this combination of biopolymers has never been explored for 3Dbioprinting. The developed bioinks were characterized in terms of their rheology, chemical structure/composition, and morphology. Furthermore, the bioprinting parameters were optimized and the cytotoxicity of the hydrogels evaluated. Rheologically, the inks presented a shear-thinning behavior, which is extremely important for 3D bioprinting applications, furthermore, an increase of three levels of magnitude in viscosity and shear stress was observed after a pre-crosslinking procedure. The inks were able to be printed with the optimal result being obtained for the ink with the highest content of NFC and prereticulated with 1% (m/v) CaCl2, which allowed the printing of up to 8 layers without losing resolution. The fully-crosslinked hydrogels were considered noncytotoxic towards HaCaT cells, however higher cell viability values were observed for the hydrogels with higher NFC content. This study can be considered a great step towards the use of pectin-NFC-based bioinks in 3Dbioprinting applications.
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spelling Novel pectin and nanocellulose based bioinks for 3D bioprinting applications3D-bioprintingHydrogelsPectinCellulose nanofibersTissue engineeringThe implementation of personalized and efficient strategies for damaged tissue/organ replacement is becoming increasingly urgent, being observed significant advancements in the field of tissue engineering in recent years, namely in 3D bioprinting of functional living tissue analogues. This automatized technology allows a wide control over the cellular environment and the overall tissue organization. To obtain a fully developed and functional tissue/organ, the bioprinting method and the bioink need to have adequate properties and functionalities, for this reason, a thoughtful choice is crucial. Recently, there have been great developments in the field of bioinks for bioprinting applications. However, the developed bioinks still present several limitations, such as weak mechanical properties, low viability, or high production costs. In this context, the objective of this dissertation is the development of a pectinnanocellulose fibers hydrogel-based bioink laden with human keratinocyte cells (HaCaT cells) for 3D bioprinting. These biopolymers were chosen due to their great potential for 3D bioprinting applications. Pectin is biodegradable, hydrophilic, and can form hydrogels in the presence of divalent cations, such as calcium. Nanocellulose has also already demonstrated its potentialities in 3Dbioprinting, revealing excellent mechanical properties and biocompatibility. However, this combination of biopolymers has never been explored for 3Dbioprinting. The developed bioinks were characterized in terms of their rheology, chemical structure/composition, and morphology. Furthermore, the bioprinting parameters were optimized and the cytotoxicity of the hydrogels evaluated. Rheologically, the inks presented a shear-thinning behavior, which is extremely important for 3D bioprinting applications, furthermore, an increase of three levels of magnitude in viscosity and shear stress was observed after a pre-crosslinking procedure. The inks were able to be printed with the optimal result being obtained for the ink with the highest content of NFC and prereticulated with 1% (m/v) CaCl2, which allowed the printing of up to 8 layers without losing resolution. The fully-crosslinked hydrogels were considered noncytotoxic towards HaCaT cells, however higher cell viability values were observed for the hydrogels with higher NFC content. This study can be considered a great step towards the use of pectin-NFC-based bioinks in 3Dbioprinting applications.A implementação de estratégias personalizadas e eficientes para a regeneração de tecidos/órgãos danificados é cada vez mais importante, enos últimos anos têm-se observado avanços significativos no campo da engenharia de tecidos, nomeadamente, na bio-impressão 3D de análogos de tecidos vivos funcionais. Esta tecnologia automatizada permite o controlo do ambiente celular e também da organização tecidular. Para que o tecido impresso seja funcional e adequado para a aplicação em causa, o método de bio-impressão e a biotinta necessitam de ter propriedades e funcionalidades adequadas, sendo a sua seleção um passo fundamental. Recentemente, tem havido um grande progresso no desenvolvimentode biotintas para aplicação em bio-impressão3D. Contudo, as biotintas desenvolvidas ainda apresentam diversas limitações, tais como fracas propriedades mecânicas, baixa viabilidade celular ou custos de produção elevados. Neste contexto, o objetivo destadissertação é o desenvolvimento de uma biotinta à base de um hidrogel de pectina e nanofibras de celulose (NFC) com incorporação de queratinócitos humanos (células HaCaT) para aplicação em bio-impressão 3D. Estes biopolímeros foram escolhidos devido às suas propriedades intrínsecas e ao seu potencial para impressão 3D. A pectina é biodegradável, hidrofílica e forma hidrogéis na presença de catiões divalentes, como o cálcio. A nanocelulose também já demonstrou as suas potencialidades, revelando excelentes propriedades mecânicas e biocompatibilidade. Contudo, a combinação de pectina e NFC nunca fui explorada no campo da bio-impressão 3D.As tintas desenvolvidas foram caraterizadas em termos da sua reologia, estrutura/composição química e morfologia. Adicionalmente, os parâmetros de impressão foram otimizados e a citotoxicidade dos hidrogéis avaliada. Reologicamente, as tintas apresentaram um comportamento pseudoplástico, que é extremamente importante para aplicações em bio-impressão 3D e, além disso, após pré-reticulação observou-se um aumento de três níveis de magnitude na viscosidade e tensão de cisalhamento. As tintas desenvolvidas têm aptidão para serem imprimidas, tendo o resultado mais promissor sido obtido para a tinta com maior quantidade de NFC e pré-reticulada com1%(m/v) de CaCl2. Nomeadamente, conseguiu-se imprimiraté8 camadas sem perder resolução. Os hidrogéis imprimidos mostraram ser não citotóxicos para as células HaCaT, contudo os valores de viabilidade celular mais elevados foram observados para os hidrogéis com maiores quantidades de nanocelulose. Este estudo considera-se como um grande passo em direção ao uso de biotintas baseadas em pectina e NFC embio-impressão3D.2023-02-17T00:00:00Z2021-02-15T00:00:00Z2021-02-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10773/30763engGuincho, Pedro Batalhainfo:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T11:59:25Zoai:ria.ua.pt:10773/30763Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:02:46.222028Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Novel pectin and nanocellulose based bioinks for 3D bioprinting applications
title Novel pectin and nanocellulose based bioinks for 3D bioprinting applications
spellingShingle Novel pectin and nanocellulose based bioinks for 3D bioprinting applications
Guincho, Pedro Batalha
3D-bioprinting
Hydrogels
Pectin
Cellulose nanofibers
Tissue engineering
title_short Novel pectin and nanocellulose based bioinks for 3D bioprinting applications
title_full Novel pectin and nanocellulose based bioinks for 3D bioprinting applications
title_fullStr Novel pectin and nanocellulose based bioinks for 3D bioprinting applications
title_full_unstemmed Novel pectin and nanocellulose based bioinks for 3D bioprinting applications
title_sort Novel pectin and nanocellulose based bioinks for 3D bioprinting applications
author Guincho, Pedro Batalha
author_facet Guincho, Pedro Batalha
author_role author
dc.contributor.author.fl_str_mv Guincho, Pedro Batalha
dc.subject.por.fl_str_mv 3D-bioprinting
Hydrogels
Pectin
Cellulose nanofibers
Tissue engineering
topic 3D-bioprinting
Hydrogels
Pectin
Cellulose nanofibers
Tissue engineering
description The implementation of personalized and efficient strategies for damaged tissue/organ replacement is becoming increasingly urgent, being observed significant advancements in the field of tissue engineering in recent years, namely in 3D bioprinting of functional living tissue analogues. This automatized technology allows a wide control over the cellular environment and the overall tissue organization. To obtain a fully developed and functional tissue/organ, the bioprinting method and the bioink need to have adequate properties and functionalities, for this reason, a thoughtful choice is crucial. Recently, there have been great developments in the field of bioinks for bioprinting applications. However, the developed bioinks still present several limitations, such as weak mechanical properties, low viability, or high production costs. In this context, the objective of this dissertation is the development of a pectinnanocellulose fibers hydrogel-based bioink laden with human keratinocyte cells (HaCaT cells) for 3D bioprinting. These biopolymers were chosen due to their great potential for 3D bioprinting applications. Pectin is biodegradable, hydrophilic, and can form hydrogels in the presence of divalent cations, such as calcium. Nanocellulose has also already demonstrated its potentialities in 3Dbioprinting, revealing excellent mechanical properties and biocompatibility. However, this combination of biopolymers has never been explored for 3Dbioprinting. The developed bioinks were characterized in terms of their rheology, chemical structure/composition, and morphology. Furthermore, the bioprinting parameters were optimized and the cytotoxicity of the hydrogels evaluated. Rheologically, the inks presented a shear-thinning behavior, which is extremely important for 3D bioprinting applications, furthermore, an increase of three levels of magnitude in viscosity and shear stress was observed after a pre-crosslinking procedure. The inks were able to be printed with the optimal result being obtained for the ink with the highest content of NFC and prereticulated with 1% (m/v) CaCl2, which allowed the printing of up to 8 layers without losing resolution. The fully-crosslinked hydrogels were considered noncytotoxic towards HaCaT cells, however higher cell viability values were observed for the hydrogels with higher NFC content. This study can be considered a great step towards the use of pectin-NFC-based bioinks in 3Dbioprinting applications.
publishDate 2021
dc.date.none.fl_str_mv 2021-02-15T00:00:00Z
2021-02-15
2023-02-17T00:00:00Z
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