Spontaneous CO release from RuII(CO)2-protein complexes in aqueous solution, cells, and mice
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2015 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10451/51756 |
Resumo: | © 2014 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
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Spontaneous CO release from RuII(CO)2-protein complexes in aqueous solution, cells, and miceCORMAlbuminCarbon monoxideMetalloproteinsProdrugs© 2014 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.We demonstrate that Ru(II)(CO)2-protein complexes, formed by the reaction of the hydrolytic decomposition products of [fac-RuCl(κ(2)-H2NCH2CO2)(CO)3] (CORM-3) with histidine residues exposed on the surface of proteins, spontaneously release CO in aqueous solution, cells, and mice. CO release was detected by mass spectrometry (MS) and confocal microscopy using a CO-responsive turn-on fluorescent probe. These findings support our hypothesis that plasma proteins act as CO carriers after in vivo administration of CORM-3. CO released from a synthetic bovine serum albumin (BSA)-Ru(II)(CO)2 complex leads to downregulation of the cytokines interleukin (IL)-6, IL-10, and tumor necrosis factor (TNF)-α in cancer cells. Finally, administration of BSA-Ru(II)(CO)2 in mice bearing a colon carcinoma tumor results in enhanced CO accumulation at the tumor. Our data suggest the use of Ru(II)(CO)2-protein complexes as viable alternatives for the safe and spatially controlled delivery of therapeutic CO in vivo.WileyRepositório da Universidade de LisboaChaves-Ferreira, MiguelAlbuquerque, Inês S.Matak-Vinkovic, DijanaCoelho, Ana C.Carvalho, Sandra M.Saraiva, Lígia M.Romão, Carlos C.Bernardes, Gonçalo J. L.2022-03-15T16:33:25Z20152015-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/51756engAngew. Chem. Int. Ed. 2015, 54, 1172-11751433-785110.1002/anie.2014093441521-3773info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-14T15:38:42ZPortal AgregadorONG |
| dc.title.none.fl_str_mv |
Spontaneous CO release from RuII(CO)2-protein complexes in aqueous solution, cells, and mice |
| title |
Spontaneous CO release from RuII(CO)2-protein complexes in aqueous solution, cells, and mice |
| spellingShingle |
Spontaneous CO release from RuII(CO)2-protein complexes in aqueous solution, cells, and mice Chaves-Ferreira, Miguel CORM Albumin Carbon monoxide Metalloproteins Prodrugs |
| title_short |
Spontaneous CO release from RuII(CO)2-protein complexes in aqueous solution, cells, and mice |
| title_full |
Spontaneous CO release from RuII(CO)2-protein complexes in aqueous solution, cells, and mice |
| title_fullStr |
Spontaneous CO release from RuII(CO)2-protein complexes in aqueous solution, cells, and mice |
| title_full_unstemmed |
Spontaneous CO release from RuII(CO)2-protein complexes in aqueous solution, cells, and mice |
| title_sort |
Spontaneous CO release from RuII(CO)2-protein complexes in aqueous solution, cells, and mice |
| author |
Chaves-Ferreira, Miguel |
| author_facet |
Chaves-Ferreira, Miguel Albuquerque, Inês S. Matak-Vinkovic, Dijana Coelho, Ana C. Carvalho, Sandra M. Saraiva, Lígia M. Romão, Carlos C. Bernardes, Gonçalo J. L. |
| author_role |
author |
| author2 |
Albuquerque, Inês S. Matak-Vinkovic, Dijana Coelho, Ana C. Carvalho, Sandra M. Saraiva, Lígia M. Romão, Carlos C. Bernardes, Gonçalo J. L. |
| author2_role |
author author author author author author author |
| dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
| dc.contributor.author.fl_str_mv |
Chaves-Ferreira, Miguel Albuquerque, Inês S. Matak-Vinkovic, Dijana Coelho, Ana C. Carvalho, Sandra M. Saraiva, Lígia M. Romão, Carlos C. Bernardes, Gonçalo J. L. |
| dc.subject.por.fl_str_mv |
CORM Albumin Carbon monoxide Metalloproteins Prodrugs |
| topic |
CORM Albumin Carbon monoxide Metalloproteins Prodrugs |
| description |
© 2014 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
| publishDate |
2015 |
| dc.date.none.fl_str_mv |
2015 2015-01-01T00:00:00Z 2022-03-15T16:33:25Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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http://hdl.handle.net/10451/51756 |
| url |
http://hdl.handle.net/10451/51756 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Angew. Chem. Int. Ed. 2015, 54, 1172-1175 1433-7851 10.1002/anie.201409344 1521-3773 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Wiley |
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Wiley |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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