Design of protein-based liquefied cell-laden capsules with bioinspired adhesion for tissue engineering

Detalhes bibliográficos
Autor(a) principal: Gomes, Maria C.
Data de Publicação: 2021
Outros Autores: Costa, Dora C. S., Oliveira, Cláudia S., Mano, João F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10773/34410
Resumo: Platforms with liquid cores are extensively explored as cell delivery vehicles for cell-based therapies and tissue engineering. However, the recurrence of synthetic materials can impair its translation into the clinic. Inspired by the adhesive proteins secreted by mussels, liquefied capsule is developed using gelatin modified with hydroxypyridinones (Gel-HOPO), a catechol analogue with oxidant-resistant properties. The protein-based liquefied macrocapsule permitted the compartmentalization of living cells by an approachable and non-time-consuming methodology resorting to i) superhydrophobic surfaces as a processing platform of hydrogel beads, ii) gelation of gelatin at temperatures < 25 °C, iii) iron coordination of the hydroxypyridinone (HOPO) moieties at physiological pH, and iv) core liquefaction at 37 °C. With the design of a proteolytically degradable shell, the possibility of encapsulating human adipose-derived mesenchymal stem cells (hASC) with and without the presence of polycaprolactone microparticles (μPCL) is evaluated. Showing prevalence toward adhesion to the inner shell wall, hASC formed a monolayer evidencing the biocompatibility and adequate mechanical properties of these platforms for proliferation, diminishing the need for μPCL as a supporting substrate. This new protein-based liquefied platform can provide biofactories devices of both fundamental and practical importance for tissue engineering and regenerative medicine or in other biotechnology fields.
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spelling Design of protein-based liquefied cell-laden capsules with bioinspired adhesion for tissue engineering3D assemblyCell encapsulationHydroxypyridinonesSuperhydrophobic surfacesPlatforms with liquid cores are extensively explored as cell delivery vehicles for cell-based therapies and tissue engineering. However, the recurrence of synthetic materials can impair its translation into the clinic. Inspired by the adhesive proteins secreted by mussels, liquefied capsule is developed using gelatin modified with hydroxypyridinones (Gel-HOPO), a catechol analogue with oxidant-resistant properties. The protein-based liquefied macrocapsule permitted the compartmentalization of living cells by an approachable and non-time-consuming methodology resorting to i) superhydrophobic surfaces as a processing platform of hydrogel beads, ii) gelation of gelatin at temperatures < 25 °C, iii) iron coordination of the hydroxypyridinone (HOPO) moieties at physiological pH, and iv) core liquefaction at 37 °C. With the design of a proteolytically degradable shell, the possibility of encapsulating human adipose-derived mesenchymal stem cells (hASC) with and without the presence of polycaprolactone microparticles (μPCL) is evaluated. Showing prevalence toward adhesion to the inner shell wall, hASC formed a monolayer evidencing the biocompatibility and adequate mechanical properties of these platforms for proliferation, diminishing the need for μPCL as a supporting substrate. This new protein-based liquefied platform can provide biofactories devices of both fundamental and practical importance for tissue engineering and regenerative medicine or in other biotechnology fields.Wiley2022-10-06T00:00:00Z2021-10-06T00:00:00Z2021-10-06info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10773/34410eng2192-264010.1002/adhm.202100782Gomes, Maria C.Costa, Dora C. S.Oliveira, Cláudia S.Mano, João F.info:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T12:06:28Zoai:ria.ua.pt:10773/34410Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:05:45.045683Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Design of protein-based liquefied cell-laden capsules with bioinspired adhesion for tissue engineering
title Design of protein-based liquefied cell-laden capsules with bioinspired adhesion for tissue engineering
spellingShingle Design of protein-based liquefied cell-laden capsules with bioinspired adhesion for tissue engineering
Gomes, Maria C.
3D assembly
Cell encapsulation
Hydroxypyridinones
Superhydrophobic surfaces
title_short Design of protein-based liquefied cell-laden capsules with bioinspired adhesion for tissue engineering
title_full Design of protein-based liquefied cell-laden capsules with bioinspired adhesion for tissue engineering
title_fullStr Design of protein-based liquefied cell-laden capsules with bioinspired adhesion for tissue engineering
title_full_unstemmed Design of protein-based liquefied cell-laden capsules with bioinspired adhesion for tissue engineering
title_sort Design of protein-based liquefied cell-laden capsules with bioinspired adhesion for tissue engineering
author Gomes, Maria C.
author_facet Gomes, Maria C.
Costa, Dora C. S.
Oliveira, Cláudia S.
Mano, João F.
author_role author
author2 Costa, Dora C. S.
Oliveira, Cláudia S.
Mano, João F.
author2_role author
author
author
dc.contributor.author.fl_str_mv Gomes, Maria C.
Costa, Dora C. S.
Oliveira, Cláudia S.
Mano, João F.
dc.subject.por.fl_str_mv 3D assembly
Cell encapsulation
Hydroxypyridinones
Superhydrophobic surfaces
topic 3D assembly
Cell encapsulation
Hydroxypyridinones
Superhydrophobic surfaces
description Platforms with liquid cores are extensively explored as cell delivery vehicles for cell-based therapies and tissue engineering. However, the recurrence of synthetic materials can impair its translation into the clinic. Inspired by the adhesive proteins secreted by mussels, liquefied capsule is developed using gelatin modified with hydroxypyridinones (Gel-HOPO), a catechol analogue with oxidant-resistant properties. The protein-based liquefied macrocapsule permitted the compartmentalization of living cells by an approachable and non-time-consuming methodology resorting to i) superhydrophobic surfaces as a processing platform of hydrogel beads, ii) gelation of gelatin at temperatures < 25 °C, iii) iron coordination of the hydroxypyridinone (HOPO) moieties at physiological pH, and iv) core liquefaction at 37 °C. With the design of a proteolytically degradable shell, the possibility of encapsulating human adipose-derived mesenchymal stem cells (hASC) with and without the presence of polycaprolactone microparticles (μPCL) is evaluated. Showing prevalence toward adhesion to the inner shell wall, hASC formed a monolayer evidencing the biocompatibility and adequate mechanical properties of these platforms for proliferation, diminishing the need for μPCL as a supporting substrate. This new protein-based liquefied platform can provide biofactories devices of both fundamental and practical importance for tissue engineering and regenerative medicine or in other biotechnology fields.
publishDate 2021
dc.date.none.fl_str_mv 2021-10-06T00:00:00Z
2021-10-06
2022-10-06T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10773/34410
url http://hdl.handle.net/10773/34410
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2192-2640
10.1002/adhm.202100782
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dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
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