Seasonal dynamics of microcystis spp. and their toxigenicity as assessed by qPCR in a temperate reservoir

Detalhes bibliográficos
Autor(a) principal: Martins, António
Data de Publicação: 2011
Outros Autores: Moreira, Cristiana, Vale, Micaela, Freitas, Marisa, Regueiras, Ana, Antunes, Agostinho, Vasconcelos, Vítor
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.22/18591
Resumo: Blooms of toxic cyanobacteria are becoming increasingly frequent, mainly due to water quality degradation. This work applied qPCR as a tool for early warning of microcystin(MC)-producer cyanobacteria and risk assessment of water supplies. Specific marker genes for cyanobacteria, Microcystis and MC-producing Microcystis, were quantified to determine the genotypic composition of the natural Microcystis population. Correlations between limnological parameters, pH, water temperature, dissolved oxygen and conductivity and MC concentrations as well as Microcystis abundance were assessed. A negative significant correlation was observed between toxic (with mcy genes) to non-toxic (without mcy genes) genotypes ratio and the overall Microcystis density. The highest proportions of toxic Microcystis genotypes were found 4-6 weeks before and 8-10 weeks after the peak of the bloom, with the lowest being observed at its peak. These results suggest positive selection of non-toxic genotypes under favorable environmental growth conditions. Significant positive correlations could be found between quantity of toxic genotypes and MC concentration, suggesting that the method applied can be useful to predict potential MC toxicity risk. No significant correlation was found between the limnological parameters measured and MC concentrations or toxic genotypes proportions indicating that other abiotic and biotic factors should be governing MC production and toxic genotypes dynamics. The qPCR method here applied is useful to rapidly estimate the potential toxicity of environmental samples and so, it may contribute to the more efficient management of water use in eutrophic systems.
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spelling Seasonal dynamics of microcystis spp. and their toxigenicity as assessed by qPCR in a temperate reservoirMicrocystinsMicrocystisqPCRWater quality monitoringWater managementBlooms of toxic cyanobacteria are becoming increasingly frequent, mainly due to water quality degradation. This work applied qPCR as a tool for early warning of microcystin(MC)-producer cyanobacteria and risk assessment of water supplies. Specific marker genes for cyanobacteria, Microcystis and MC-producing Microcystis, were quantified to determine the genotypic composition of the natural Microcystis population. Correlations between limnological parameters, pH, water temperature, dissolved oxygen and conductivity and MC concentrations as well as Microcystis abundance were assessed. A negative significant correlation was observed between toxic (with mcy genes) to non-toxic (without mcy genes) genotypes ratio and the overall Microcystis density. The highest proportions of toxic Microcystis genotypes were found 4-6 weeks before and 8-10 weeks after the peak of the bloom, with the lowest being observed at its peak. These results suggest positive selection of non-toxic genotypes under favorable environmental growth conditions. Significant positive correlations could be found between quantity of toxic genotypes and MC concentration, suggesting that the method applied can be useful to predict potential MC toxicity risk. No significant correlation was found between the limnological parameters measured and MC concentrations or toxic genotypes proportions indicating that other abiotic and biotic factors should be governing MC production and toxic genotypes dynamics. The qPCR method here applied is useful to rapidly estimate the potential toxicity of environmental samples and so, it may contribute to the more efficient management of water use in eutrophic systems.MDPIRepositório Científico do Instituto Politécnico do PortoMartins, AntónioMoreira, CristianaVale, MicaelaFreitas, MarisaRegueiras, AnaAntunes, AgostinhoVasconcelos, Vítor2021-09-28T08:57:08Z2011-092011-09-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.22/18591engMartins, A., Moreira, C., Vale, M., Freitas, M., Regueiras, A., Antunes, A., & Vasconcelos, V. (2011). Seasonal dynamics of Microcystis spp. and their toxigenicity as assessed by qPCR in a temperate reservoir. Marine Drugs, 9(10), 1715-1730. https://www.mdpi.com/1660-3397/9/10/17151660-339710.3390/md9101715info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-13T13:10:21Zoai:recipp.ipp.pt:10400.22/18591Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:38:08.318236Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Seasonal dynamics of microcystis spp. and their toxigenicity as assessed by qPCR in a temperate reservoir
title Seasonal dynamics of microcystis spp. and their toxigenicity as assessed by qPCR in a temperate reservoir
spellingShingle Seasonal dynamics of microcystis spp. and their toxigenicity as assessed by qPCR in a temperate reservoir
Martins, António
Microcystins
Microcystis
qPCR
Water quality monitoring
Water management
title_short Seasonal dynamics of microcystis spp. and their toxigenicity as assessed by qPCR in a temperate reservoir
title_full Seasonal dynamics of microcystis spp. and their toxigenicity as assessed by qPCR in a temperate reservoir
title_fullStr Seasonal dynamics of microcystis spp. and their toxigenicity as assessed by qPCR in a temperate reservoir
title_full_unstemmed Seasonal dynamics of microcystis spp. and their toxigenicity as assessed by qPCR in a temperate reservoir
title_sort Seasonal dynamics of microcystis spp. and their toxigenicity as assessed by qPCR in a temperate reservoir
author Martins, António
author_facet Martins, António
Moreira, Cristiana
Vale, Micaela
Freitas, Marisa
Regueiras, Ana
Antunes, Agostinho
Vasconcelos, Vítor
author_role author
author2 Moreira, Cristiana
Vale, Micaela
Freitas, Marisa
Regueiras, Ana
Antunes, Agostinho
Vasconcelos, Vítor
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Politécnico do Porto
dc.contributor.author.fl_str_mv Martins, António
Moreira, Cristiana
Vale, Micaela
Freitas, Marisa
Regueiras, Ana
Antunes, Agostinho
Vasconcelos, Vítor
dc.subject.por.fl_str_mv Microcystins
Microcystis
qPCR
Water quality monitoring
Water management
topic Microcystins
Microcystis
qPCR
Water quality monitoring
Water management
description Blooms of toxic cyanobacteria are becoming increasingly frequent, mainly due to water quality degradation. This work applied qPCR as a tool for early warning of microcystin(MC)-producer cyanobacteria and risk assessment of water supplies. Specific marker genes for cyanobacteria, Microcystis and MC-producing Microcystis, were quantified to determine the genotypic composition of the natural Microcystis population. Correlations between limnological parameters, pH, water temperature, dissolved oxygen and conductivity and MC concentrations as well as Microcystis abundance were assessed. A negative significant correlation was observed between toxic (with mcy genes) to non-toxic (without mcy genes) genotypes ratio and the overall Microcystis density. The highest proportions of toxic Microcystis genotypes were found 4-6 weeks before and 8-10 weeks after the peak of the bloom, with the lowest being observed at its peak. These results suggest positive selection of non-toxic genotypes under favorable environmental growth conditions. Significant positive correlations could be found between quantity of toxic genotypes and MC concentration, suggesting that the method applied can be useful to predict potential MC toxicity risk. No significant correlation was found between the limnological parameters measured and MC concentrations or toxic genotypes proportions indicating that other abiotic and biotic factors should be governing MC production and toxic genotypes dynamics. The qPCR method here applied is useful to rapidly estimate the potential toxicity of environmental samples and so, it may contribute to the more efficient management of water use in eutrophic systems.
publishDate 2011
dc.date.none.fl_str_mv 2011-09
2011-09-01T00:00:00Z
2021-09-28T08:57:08Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.22/18591
url http://hdl.handle.net/10400.22/18591
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Martins, A., Moreira, C., Vale, M., Freitas, M., Regueiras, A., Antunes, A., & Vasconcelos, V. (2011). Seasonal dynamics of Microcystis spp. and their toxigenicity as assessed by qPCR in a temperate reservoir. Marine Drugs, 9(10), 1715-1730. https://www.mdpi.com/1660-3397/9/10/1715
1660-3397
10.3390/md9101715
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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