Seasonal dynamics of microcystis spp. and their toxigenicity as assessed by qPCR in a temperate reservoir
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.22/18591 |
Resumo: | Blooms of toxic cyanobacteria are becoming increasingly frequent, mainly due to water quality degradation. This work applied qPCR as a tool for early warning of microcystin(MC)-producer cyanobacteria and risk assessment of water supplies. Specific marker genes for cyanobacteria, Microcystis and MC-producing Microcystis, were quantified to determine the genotypic composition of the natural Microcystis population. Correlations between limnological parameters, pH, water temperature, dissolved oxygen and conductivity and MC concentrations as well as Microcystis abundance were assessed. A negative significant correlation was observed between toxic (with mcy genes) to non-toxic (without mcy genes) genotypes ratio and the overall Microcystis density. The highest proportions of toxic Microcystis genotypes were found 4-6 weeks before and 8-10 weeks after the peak of the bloom, with the lowest being observed at its peak. These results suggest positive selection of non-toxic genotypes under favorable environmental growth conditions. Significant positive correlations could be found between quantity of toxic genotypes and MC concentration, suggesting that the method applied can be useful to predict potential MC toxicity risk. No significant correlation was found between the limnological parameters measured and MC concentrations or toxic genotypes proportions indicating that other abiotic and biotic factors should be governing MC production and toxic genotypes dynamics. The qPCR method here applied is useful to rapidly estimate the potential toxicity of environmental samples and so, it may contribute to the more efficient management of water use in eutrophic systems. |
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Seasonal dynamics of microcystis spp. and their toxigenicity as assessed by qPCR in a temperate reservoirMicrocystinsMicrocystisqPCRWater quality monitoringWater managementBlooms of toxic cyanobacteria are becoming increasingly frequent, mainly due to water quality degradation. This work applied qPCR as a tool for early warning of microcystin(MC)-producer cyanobacteria and risk assessment of water supplies. Specific marker genes for cyanobacteria, Microcystis and MC-producing Microcystis, were quantified to determine the genotypic composition of the natural Microcystis population. Correlations between limnological parameters, pH, water temperature, dissolved oxygen and conductivity and MC concentrations as well as Microcystis abundance were assessed. A negative significant correlation was observed between toxic (with mcy genes) to non-toxic (without mcy genes) genotypes ratio and the overall Microcystis density. The highest proportions of toxic Microcystis genotypes were found 4-6 weeks before and 8-10 weeks after the peak of the bloom, with the lowest being observed at its peak. These results suggest positive selection of non-toxic genotypes under favorable environmental growth conditions. Significant positive correlations could be found between quantity of toxic genotypes and MC concentration, suggesting that the method applied can be useful to predict potential MC toxicity risk. No significant correlation was found between the limnological parameters measured and MC concentrations or toxic genotypes proportions indicating that other abiotic and biotic factors should be governing MC production and toxic genotypes dynamics. The qPCR method here applied is useful to rapidly estimate the potential toxicity of environmental samples and so, it may contribute to the more efficient management of water use in eutrophic systems.MDPIRepositório Científico do Instituto Politécnico do PortoMartins, AntónioMoreira, CristianaVale, MicaelaFreitas, MarisaRegueiras, AnaAntunes, AgostinhoVasconcelos, Vítor2021-09-28T08:57:08Z2011-092011-09-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.22/18591engMartins, A., Moreira, C., Vale, M., Freitas, M., Regueiras, A., Antunes, A., & Vasconcelos, V. (2011). Seasonal dynamics of Microcystis spp. and their toxigenicity as assessed by qPCR in a temperate reservoir. Marine Drugs, 9(10), 1715-1730. https://www.mdpi.com/1660-3397/9/10/17151660-339710.3390/md9101715info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-13T13:10:21Zoai:recipp.ipp.pt:10400.22/18591Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:38:08.318236Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Seasonal dynamics of microcystis spp. and their toxigenicity as assessed by qPCR in a temperate reservoir |
title |
Seasonal dynamics of microcystis spp. and their toxigenicity as assessed by qPCR in a temperate reservoir |
spellingShingle |
Seasonal dynamics of microcystis spp. and their toxigenicity as assessed by qPCR in a temperate reservoir Martins, António Microcystins Microcystis qPCR Water quality monitoring Water management |
title_short |
Seasonal dynamics of microcystis spp. and their toxigenicity as assessed by qPCR in a temperate reservoir |
title_full |
Seasonal dynamics of microcystis spp. and their toxigenicity as assessed by qPCR in a temperate reservoir |
title_fullStr |
Seasonal dynamics of microcystis spp. and their toxigenicity as assessed by qPCR in a temperate reservoir |
title_full_unstemmed |
Seasonal dynamics of microcystis spp. and their toxigenicity as assessed by qPCR in a temperate reservoir |
title_sort |
Seasonal dynamics of microcystis spp. and their toxigenicity as assessed by qPCR in a temperate reservoir |
author |
Martins, António |
author_facet |
Martins, António Moreira, Cristiana Vale, Micaela Freitas, Marisa Regueiras, Ana Antunes, Agostinho Vasconcelos, Vítor |
author_role |
author |
author2 |
Moreira, Cristiana Vale, Micaela Freitas, Marisa Regueiras, Ana Antunes, Agostinho Vasconcelos, Vítor |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Politécnico do Porto |
dc.contributor.author.fl_str_mv |
Martins, António Moreira, Cristiana Vale, Micaela Freitas, Marisa Regueiras, Ana Antunes, Agostinho Vasconcelos, Vítor |
dc.subject.por.fl_str_mv |
Microcystins Microcystis qPCR Water quality monitoring Water management |
topic |
Microcystins Microcystis qPCR Water quality monitoring Water management |
description |
Blooms of toxic cyanobacteria are becoming increasingly frequent, mainly due to water quality degradation. This work applied qPCR as a tool for early warning of microcystin(MC)-producer cyanobacteria and risk assessment of water supplies. Specific marker genes for cyanobacteria, Microcystis and MC-producing Microcystis, were quantified to determine the genotypic composition of the natural Microcystis population. Correlations between limnological parameters, pH, water temperature, dissolved oxygen and conductivity and MC concentrations as well as Microcystis abundance were assessed. A negative significant correlation was observed between toxic (with mcy genes) to non-toxic (without mcy genes) genotypes ratio and the overall Microcystis density. The highest proportions of toxic Microcystis genotypes were found 4-6 weeks before and 8-10 weeks after the peak of the bloom, with the lowest being observed at its peak. These results suggest positive selection of non-toxic genotypes under favorable environmental growth conditions. Significant positive correlations could be found between quantity of toxic genotypes and MC concentration, suggesting that the method applied can be useful to predict potential MC toxicity risk. No significant correlation was found between the limnological parameters measured and MC concentrations or toxic genotypes proportions indicating that other abiotic and biotic factors should be governing MC production and toxic genotypes dynamics. The qPCR method here applied is useful to rapidly estimate the potential toxicity of environmental samples and so, it may contribute to the more efficient management of water use in eutrophic systems. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-09 2011-09-01T00:00:00Z 2021-09-28T08:57:08Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.22/18591 |
url |
http://hdl.handle.net/10400.22/18591 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Martins, A., Moreira, C., Vale, M., Freitas, M., Regueiras, A., Antunes, A., & Vasconcelos, V. (2011). Seasonal dynamics of Microcystis spp. and their toxigenicity as assessed by qPCR in a temperate reservoir. Marine Drugs, 9(10), 1715-1730. https://www.mdpi.com/1660-3397/9/10/1715 1660-3397 10.3390/md9101715 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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