Attomolar detection of hepatitis C virus core protein powered by molecular antenna-like effect in a graphene field-effect aptasensor
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/81265 |
Resumo: | Biosensors based on graphene field-effect transistors have become a promising tool for detecting a broad range of analytes. However, their performance is substantially affected by the functionalization protocol. In this work, we use a controlled in-vacuum physical method for the covalent functionalization of graphene to construct ultrasensitive aptamer-based biosensors (aptasensors) able to detect hepatitis C virus core protein. These devices are highly specific and robust, achieving attomolar detection of the viral protein in human blood plasma. Such an improved sensitivity is rationalized by theoretical calculations showing that induced polarization at the graphene interface, caused by the proximity of covalently bound molecular probe, modulates the charge balance at the graphene/aptamer interface. This charge balance causes a net shift of the Dirac cone providing enhanced sensitivity for the attomolar detection of the target proteins. Such an unexpected effect paves the way for using this kind of graphene-based functionalized platforms for ultrasensitive and real-time diagnostics of different diseases. |
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Attomolar detection of hepatitis C virus core protein powered by molecular antenna-like effect in a graphene field-effect aptasensorGrapheneFET-biosensorsAptamersCovalent functionalizationHCVEngenharia e Tecnologia::NanotecnologiaScience & TechnologyBiosensors based on graphene field-effect transistors have become a promising tool for detecting a broad range of analytes. However, their performance is substantially affected by the functionalization protocol. In this work, we use a controlled in-vacuum physical method for the covalent functionalization of graphene to construct ultrasensitive aptamer-based biosensors (aptasensors) able to detect hepatitis C virus core protein. These devices are highly specific and robust, achieving attomolar detection of the viral protein in human blood plasma. Such an improved sensitivity is rationalized by theoretical calculations showing that induced polarization at the graphene interface, caused by the proximity of covalently bound molecular probe, modulates the charge balance at the graphene/aptamer interface. This charge balance causes a net shift of the Dirac cone providing enhanced sensitivity for the attomolar detection of the target proteins. Such an unexpected effect paves the way for using this kind of graphene-based functionalized platforms for ultrasensitive and real-time diagnostics of different diseases.This work has been supported by the EU Graphene Flagship funding (Grant Graphene Core3 881603), the Ministerio de Ciencia e Innovacion of Spain: PID2020-113142RB-C21, the European Structural Funds via FotoArt-CM project (P2018/NMT-4367) and the Portuguese Foundation for Science and Technology (FCT) via the Strategic Funding UIDB/04650/2020. Work at CAB was funded by the Spanish Ministerio de Ciencia e Innovacion (MICINN) grant no. PID2019-104903RB-I00 and the Spanish Agencia Estatal de Investigacion (AEI) Project no. MDM-2017-0737 - Unidad de Excelencia "Maria de Maeztu," and it also benefits from the interdisciplinary framework provided by CSIC through "LifeHUB.CSIC" initiative (PIE 202120E047-CONEXIONES-LIFE). CIBERehd is funded by Instituto de Salud Carlos III (ISCIII). A.N. is supported by the predoctoral fellowship PRE-CAB-BIOMOLECULAS 2 from INTA. B.T-V. is supported by the predoctoral fellowship TS17/16 from INTA and by the CSIC "Garantia Juvenil" contract CAM19_PRE_-CAB_001 funded by Comunidad Autonoma de Madrid (CAM). FCT supports T.D. and P.C. under Ph.D. grants SFRH/BD/08181/2020 and SFRH/BD/128579/2017. M.M. would like to thank Comunidad de Madrid for the predoctoral grant IND2020/BIO-17523. P.A. and C.B. also acknowledge the support provided by La Caixa Foundation through Project LCF/PR/HR21/52410023. L. V. would like to thank Comunidad Autonoma de Madrid (TRANSNANOAVANSENS program: S2018-NMT- 4349) and E.V. Garcia-Frutos for her assistance during the AFM experimentsElsevierUniversidade do MinhoPalacio, IreneMoreno, MiguelNáñez, AlmudenaPurwidyantri, AgnesDomingues, TelmaCabral, Patrícia D.Borme, JérômeMarciello, MarziaMendieta-Moreno, Jesús IgnacioTorres-Vázquez, BeatrizMartínez, José IgnacioLópez, María FranciscaGarcía-Hernández, MarVázquez, LuisJelínek, PavelAlpuim, P.Briones, CarlosMartín-Gago, José Ángel20232023-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfapplication/pdfhttps://hdl.handle.net/1822/81265engPalacio, I., Moreno, M., Náñez, A., Purwidyantri, A., Domingues, T., Cabral, P. D., … Martín-Gago, J. Á. (2023, February). Attomolar detection of hepatitis C virus core protein powered by molecular antenna-like effect in a graphene field-effect aptasensor. Biosensors and Bioelectronics. Elsevier BV. http://doi.org/10.1016/j.bios.2022.1150060956-566310.1016/j.bios.2022.11500636538869https://www.sciencedirect.com/science/article/pii/S0956566322010466info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-12-23T01:27:21Zoai:repositorium.sdum.uminho.pt:1822/81265Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:47:02.640368Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Attomolar detection of hepatitis C virus core protein powered by molecular antenna-like effect in a graphene field-effect aptasensor |
title |
Attomolar detection of hepatitis C virus core protein powered by molecular antenna-like effect in a graphene field-effect aptasensor |
spellingShingle |
Attomolar detection of hepatitis C virus core protein powered by molecular antenna-like effect in a graphene field-effect aptasensor Palacio, Irene Graphene FET-biosensors Aptamers Covalent functionalization HCV Engenharia e Tecnologia::Nanotecnologia Science & Technology |
title_short |
Attomolar detection of hepatitis C virus core protein powered by molecular antenna-like effect in a graphene field-effect aptasensor |
title_full |
Attomolar detection of hepatitis C virus core protein powered by molecular antenna-like effect in a graphene field-effect aptasensor |
title_fullStr |
Attomolar detection of hepatitis C virus core protein powered by molecular antenna-like effect in a graphene field-effect aptasensor |
title_full_unstemmed |
Attomolar detection of hepatitis C virus core protein powered by molecular antenna-like effect in a graphene field-effect aptasensor |
title_sort |
Attomolar detection of hepatitis C virus core protein powered by molecular antenna-like effect in a graphene field-effect aptasensor |
author |
Palacio, Irene |
author_facet |
Palacio, Irene Moreno, Miguel Náñez, Almudena Purwidyantri, Agnes Domingues, Telma Cabral, Patrícia D. Borme, Jérôme Marciello, Marzia Mendieta-Moreno, Jesús Ignacio Torres-Vázquez, Beatriz Martínez, José Ignacio López, María Francisca García-Hernández, Mar Vázquez, Luis Jelínek, Pavel Alpuim, P. Briones, Carlos Martín-Gago, José Ángel |
author_role |
author |
author2 |
Moreno, Miguel Náñez, Almudena Purwidyantri, Agnes Domingues, Telma Cabral, Patrícia D. Borme, Jérôme Marciello, Marzia Mendieta-Moreno, Jesús Ignacio Torres-Vázquez, Beatriz Martínez, José Ignacio López, María Francisca García-Hernández, Mar Vázquez, Luis Jelínek, Pavel Alpuim, P. Briones, Carlos Martín-Gago, José Ángel |
author2_role |
author author author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Palacio, Irene Moreno, Miguel Náñez, Almudena Purwidyantri, Agnes Domingues, Telma Cabral, Patrícia D. Borme, Jérôme Marciello, Marzia Mendieta-Moreno, Jesús Ignacio Torres-Vázquez, Beatriz Martínez, José Ignacio López, María Francisca García-Hernández, Mar Vázquez, Luis Jelínek, Pavel Alpuim, P. Briones, Carlos Martín-Gago, José Ángel |
dc.subject.por.fl_str_mv |
Graphene FET-biosensors Aptamers Covalent functionalization HCV Engenharia e Tecnologia::Nanotecnologia Science & Technology |
topic |
Graphene FET-biosensors Aptamers Covalent functionalization HCV Engenharia e Tecnologia::Nanotecnologia Science & Technology |
description |
Biosensors based on graphene field-effect transistors have become a promising tool for detecting a broad range of analytes. However, their performance is substantially affected by the functionalization protocol. In this work, we use a controlled in-vacuum physical method for the covalent functionalization of graphene to construct ultrasensitive aptamer-based biosensors (aptasensors) able to detect hepatitis C virus core protein. These devices are highly specific and robust, achieving attomolar detection of the viral protein in human blood plasma. Such an improved sensitivity is rationalized by theoretical calculations showing that induced polarization at the graphene interface, caused by the proximity of covalently bound molecular probe, modulates the charge balance at the graphene/aptamer interface. This charge balance causes a net shift of the Dirac cone providing enhanced sensitivity for the attomolar detection of the target proteins. Such an unexpected effect paves the way for using this kind of graphene-based functionalized platforms for ultrasensitive and real-time diagnostics of different diseases. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023 2023-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/81265 |
url |
https://hdl.handle.net/1822/81265 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Palacio, I., Moreno, M., Náñez, A., Purwidyantri, A., Domingues, T., Cabral, P. D., … Martín-Gago, J. Á. (2023, February). Attomolar detection of hepatitis C virus core protein powered by molecular antenna-like effect in a graphene field-effect aptasensor. Biosensors and Bioelectronics. Elsevier BV. http://doi.org/10.1016/j.bios.2022.115006 0956-5663 10.1016/j.bios.2022.115006 36538869 https://www.sciencedirect.com/science/article/pii/S0956566322010466 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799132227881140224 |