Characterization of the bacterial communities of aerobic granules in a 2-fluorophenol degrading process
Main Author: | |
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Publication Date: | 2015 |
Other Authors: | , |
Format: | Article |
Language: | eng |
Source: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Download full: | http://hdl.handle.net/10400.14/19905 |
Summary: | Aerobic granular sludge constitutes a novel technology for wastewater treatment. This study focused on the effect of 2-fluorophenol (2-FP) shock loadings on the microbial community diversity present in aerobic granules before and after inoculation with a bacterial strain able to degrade 2-FP, Rhodococcus sp. strain FP1. After bioaugmentation, apart from strain FP1, five culturable bacteria were isolated from the 2-FP degrading granules, belonging to the following genera: Serratia, Chryseobacterium, Xanthomonas, Pimelobacter and Rhodococcus. The latter two isolates are able to degrade 2-FP. Changes in the aerobic granules’ bacterial communities related to 2-FP shock loadings were examined using denaturing gradient gel electrophoresis (DGGE) of 16S rRNA gene pool. Numerical analysis of the DGGE profiles showed high diversity with an even distribution of species. Based on cluster analysis of the DGGE profiles, the bacterial communities present in the aerobic granules changes were related to the sampling time and the 2-FP concentration fed. |
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Characterization of the bacterial communities of aerobic granules in a 2-fluorophenol degrading processAerobic granular sludge2-Fluorophenol (2-FP)Rhodococcus sp. strain FP1Denaturing gradient gel electrophoresis (DGGE)Microbial community diversityAerobic granular sludge constitutes a novel technology for wastewater treatment. This study focused on the effect of 2-fluorophenol (2-FP) shock loadings on the microbial community diversity present in aerobic granules before and after inoculation with a bacterial strain able to degrade 2-FP, Rhodococcus sp. strain FP1. After bioaugmentation, apart from strain FP1, five culturable bacteria were isolated from the 2-FP degrading granules, belonging to the following genera: Serratia, Chryseobacterium, Xanthomonas, Pimelobacter and Rhodococcus. The latter two isolates are able to degrade 2-FP. Changes in the aerobic granules’ bacterial communities related to 2-FP shock loadings were examined using denaturing gradient gel electrophoresis (DGGE) of 16S rRNA gene pool. Numerical analysis of the DGGE profiles showed high diversity with an even distribution of species. Based on cluster analysis of the DGGE profiles, the bacterial communities present in the aerobic granules changes were related to the sampling time and the 2-FP concentration fed.ElsevierVeritati - Repositório Institucional da Universidade Católica PortuguesaDuque, Anouk F.Bessa, Vânia S.Castro, Paula M. L.2016-04-21T14:41:44Z20152015-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/19905engDUQUE, Anouk F.; BESSA, Vânia S.; CASTRO, Paula M.L. - Characterization of the bacterial communities of aerobic granules in a 2-fluorophenol degrading process. Biotechnology Reports. ISSN 2215-017X. Vol. 5 (2015), p. 98–1042215-017X10.1016/j.btre.2014.12.00784926356715PMC546619728626688info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-08-15T01:41:13Zoai:repositorio.ucp.pt:10400.14/19905Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:15:59.830206Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Characterization of the bacterial communities of aerobic granules in a 2-fluorophenol degrading process |
title |
Characterization of the bacterial communities of aerobic granules in a 2-fluorophenol degrading process |
spellingShingle |
Characterization of the bacterial communities of aerobic granules in a 2-fluorophenol degrading process Duque, Anouk F. Aerobic granular sludge 2-Fluorophenol (2-FP) Rhodococcus sp. strain FP1 Denaturing gradient gel electrophoresis (DGGE) Microbial community diversity |
title_short |
Characterization of the bacterial communities of aerobic granules in a 2-fluorophenol degrading process |
title_full |
Characterization of the bacterial communities of aerobic granules in a 2-fluorophenol degrading process |
title_fullStr |
Characterization of the bacterial communities of aerobic granules in a 2-fluorophenol degrading process |
title_full_unstemmed |
Characterization of the bacterial communities of aerobic granules in a 2-fluorophenol degrading process |
title_sort |
Characterization of the bacterial communities of aerobic granules in a 2-fluorophenol degrading process |
author |
Duque, Anouk F. |
author_facet |
Duque, Anouk F. Bessa, Vânia S. Castro, Paula M. L. |
author_role |
author |
author2 |
Bessa, Vânia S. Castro, Paula M. L. |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Veritati - Repositório Institucional da Universidade Católica Portuguesa |
dc.contributor.author.fl_str_mv |
Duque, Anouk F. Bessa, Vânia S. Castro, Paula M. L. |
dc.subject.por.fl_str_mv |
Aerobic granular sludge 2-Fluorophenol (2-FP) Rhodococcus sp. strain FP1 Denaturing gradient gel electrophoresis (DGGE) Microbial community diversity |
topic |
Aerobic granular sludge 2-Fluorophenol (2-FP) Rhodococcus sp. strain FP1 Denaturing gradient gel electrophoresis (DGGE) Microbial community diversity |
description |
Aerobic granular sludge constitutes a novel technology for wastewater treatment. This study focused on the effect of 2-fluorophenol (2-FP) shock loadings on the microbial community diversity present in aerobic granules before and after inoculation with a bacterial strain able to degrade 2-FP, Rhodococcus sp. strain FP1. After bioaugmentation, apart from strain FP1, five culturable bacteria were isolated from the 2-FP degrading granules, belonging to the following genera: Serratia, Chryseobacterium, Xanthomonas, Pimelobacter and Rhodococcus. The latter two isolates are able to degrade 2-FP. Changes in the aerobic granules’ bacterial communities related to 2-FP shock loadings were examined using denaturing gradient gel electrophoresis (DGGE) of 16S rRNA gene pool. Numerical analysis of the DGGE profiles showed high diversity with an even distribution of species. Based on cluster analysis of the DGGE profiles, the bacterial communities present in the aerobic granules changes were related to the sampling time and the 2-FP concentration fed. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015 2015-01-01T00:00:00Z 2016-04-21T14:41:44Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.14/19905 |
url |
http://hdl.handle.net/10400.14/19905 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
DUQUE, Anouk F.; BESSA, Vânia S.; CASTRO, Paula M.L. - Characterization of the bacterial communities of aerobic granules in a 2-fluorophenol degrading process. Biotechnology Reports. ISSN 2215-017X. Vol. 5 (2015), p. 98–104 2215-017X 10.1016/j.btre.2014.12.007 84926356715 PMC5466197 28626688 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799131842604957696 |