ELISA as an effective tool to determine spatial and seasonal occurrence of emerging contaminants in the aquatic environment
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10773/28420 |
Resumo: | During the last two decades, studies related to the occurrence and fate of emerging contaminants in the aquatic environment have received great attention from the international scientific community. The presence of these compounds is particularly important since they are known to induce adverse effects in aquatic environments, even at extremely low concentrations. This work aimed to apply a simple and effective methodology, as enzyme-linked immunosorbent assay (ELISA), in the monitoring of 17α-ethinylestradiol (EE2) and 17β-estradiol (E2) (a synthetic and a natural hormone, respectively), carbamazepine (CBZ, an antiepileptic), cetirizine (CET, an antihistamine) and caffeine (CAF, a stimulant) in water matrices with differing salinity and organic matter contents. ELISA was proven to be a valid and practical tool, especially for screening purposes in contrast to the traditional chromatographic techniques which are prohibitively expensive for an application on a broader base. The main originality of this work was to establish seasonal and spatial effects on the occurrence of the referred contaminants by using the effectiveness of ELISA to screen those compounds in samples with different characteristics. This work reports both the seasonal and spatial quantification of the referred contaminants in the aquatic environment of the central region of Portugal, with concentrations ranging as follows: 5-87 ng L-1, for E2, 2-17 ng L-1, for EE2, 10-1290 ng L-1, for CBZ, 10-190 ng L-1, for CET and 62-6400 ng L-1, for CAF. |
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ELISA as an effective tool to determine spatial and seasonal occurrence of emerging contaminants in the aquatic environmentELISADLLMEAquatic environmentEmerging contaminantsAnthropogenic markersDuring the last two decades, studies related to the occurrence and fate of emerging contaminants in the aquatic environment have received great attention from the international scientific community. The presence of these compounds is particularly important since they are known to induce adverse effects in aquatic environments, even at extremely low concentrations. This work aimed to apply a simple and effective methodology, as enzyme-linked immunosorbent assay (ELISA), in the monitoring of 17α-ethinylestradiol (EE2) and 17β-estradiol (E2) (a synthetic and a natural hormone, respectively), carbamazepine (CBZ, an antiepileptic), cetirizine (CET, an antihistamine) and caffeine (CAF, a stimulant) in water matrices with differing salinity and organic matter contents. ELISA was proven to be a valid and practical tool, especially for screening purposes in contrast to the traditional chromatographic techniques which are prohibitively expensive for an application on a broader base. The main originality of this work was to establish seasonal and spatial effects on the occurrence of the referred contaminants by using the effectiveness of ELISA to screen those compounds in samples with different characteristics. This work reports both the seasonal and spatial quantification of the referred contaminants in the aquatic environment of the central region of Portugal, with concentrations ranging as follows: 5-87 ng L-1, for E2, 2-17 ng L-1, for EE2, 10-1290 ng L-1, for CBZ, 10-190 ng L-1, for CET and 62-6400 ng L-1, for CAF.Royal Society of Chemistry2020-05-07T14:24:09Z2020-05-07T00:00:00Z2020-05-07info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/vnd.openxmlformats-officedocument.wordprocessingml.documenthttp://hdl.handle.net/10773/28420eng1759-966010.1039/D0AY00426JSilva, Carla PatríciaCarvalho, TâniaSchneider, Rudolf J.Esteves, Valdemar I.Lima, Diana L. D.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-17T04:04:23ZPortal AgregadorONG |
dc.title.none.fl_str_mv |
ELISA as an effective tool to determine spatial and seasonal occurrence of emerging contaminants in the aquatic environment |
title |
ELISA as an effective tool to determine spatial and seasonal occurrence of emerging contaminants in the aquatic environment |
spellingShingle |
ELISA as an effective tool to determine spatial and seasonal occurrence of emerging contaminants in the aquatic environment Silva, Carla Patrícia ELISA DLLME Aquatic environment Emerging contaminants Anthropogenic markers |
title_short |
ELISA as an effective tool to determine spatial and seasonal occurrence of emerging contaminants in the aquatic environment |
title_full |
ELISA as an effective tool to determine spatial and seasonal occurrence of emerging contaminants in the aquatic environment |
title_fullStr |
ELISA as an effective tool to determine spatial and seasonal occurrence of emerging contaminants in the aquatic environment |
title_full_unstemmed |
ELISA as an effective tool to determine spatial and seasonal occurrence of emerging contaminants in the aquatic environment |
title_sort |
ELISA as an effective tool to determine spatial and seasonal occurrence of emerging contaminants in the aquatic environment |
author |
Silva, Carla Patrícia |
author_facet |
Silva, Carla Patrícia Carvalho, Tânia Schneider, Rudolf J. Esteves, Valdemar I. Lima, Diana L. D. |
author_role |
author |
author2 |
Carvalho, Tânia Schneider, Rudolf J. Esteves, Valdemar I. Lima, Diana L. D. |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Silva, Carla Patrícia Carvalho, Tânia Schneider, Rudolf J. Esteves, Valdemar I. Lima, Diana L. D. |
dc.subject.por.fl_str_mv |
ELISA DLLME Aquatic environment Emerging contaminants Anthropogenic markers |
topic |
ELISA DLLME Aquatic environment Emerging contaminants Anthropogenic markers |
description |
During the last two decades, studies related to the occurrence and fate of emerging contaminants in the aquatic environment have received great attention from the international scientific community. The presence of these compounds is particularly important since they are known to induce adverse effects in aquatic environments, even at extremely low concentrations. This work aimed to apply a simple and effective methodology, as enzyme-linked immunosorbent assay (ELISA), in the monitoring of 17α-ethinylestradiol (EE2) and 17β-estradiol (E2) (a synthetic and a natural hormone, respectively), carbamazepine (CBZ, an antiepileptic), cetirizine (CET, an antihistamine) and caffeine (CAF, a stimulant) in water matrices with differing salinity and organic matter contents. ELISA was proven to be a valid and practical tool, especially for screening purposes in contrast to the traditional chromatographic techniques which are prohibitively expensive for an application on a broader base. The main originality of this work was to establish seasonal and spatial effects on the occurrence of the referred contaminants by using the effectiveness of ELISA to screen those compounds in samples with different characteristics. This work reports both the seasonal and spatial quantification of the referred contaminants in the aquatic environment of the central region of Portugal, with concentrations ranging as follows: 5-87 ng L-1, for E2, 2-17 ng L-1, for EE2, 10-1290 ng L-1, for CBZ, 10-190 ng L-1, for CET and 62-6400 ng L-1, for CAF. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-05-07T14:24:09Z 2020-05-07T00:00:00Z 2020-05-07 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10773/28420 |
url |
http://hdl.handle.net/10773/28420 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1759-9660 10.1039/D0AY00426J |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/vnd.openxmlformats-officedocument.wordprocessingml.document |
dc.publisher.none.fl_str_mv |
Royal Society of Chemistry |
publisher.none.fl_str_mv |
Royal Society of Chemistry |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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1777303558396313600 |