Pseudomonas aeruginosa PAO 1 in vitro timekill kinetics using single phages and phage formulationsmodulating death, adaptation, and resistance
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/73778 |
Resumo: | Pseudomonas aeruginosa is responsible for nosocomial and chronic infections in healthcare settings. The major challenge in treating P. aeruginosa-related diseases is its remarkable capacity for antibiotic resistance development. Bacteriophage (phage) therapy is regarded as a possible alternative that has, for years, attracted attention for fighting multidrug-resistant infections. In this work, we characterized five phages showing different lytic spectrums towards clinical isolates. Two of these phages were isolated from the Russian Microgen Sextaphage formulation and belong to the Phikmvviruses, while three Pbunaviruses were isolated from sewage. Different phage formulations for the treatment of P. aeruginosa PAO1 resulted in diversified timekill outcomes. The best result was obtained with a formulation with all phages, prompting a lower frequency of resistant variants and considerable alterations in cell motility, resulting in a loss of 73.7% in swimming motility and a 79% change in swarming motility. These alterations diminished the virulence of the phage-resisting phenotypes but promoted their growth since most became insensitive to a single or even all phages. However, not all combinations drove to enhanced cell killings due to the competition and loss of receptors. This study highlights that more caution is needed when developing cocktail formulations to maximize phage therapy efficacy. Selecting phages for formulations should consider the emergence of phage-resistant bacteria and whether the formulations are intended for short-term or extended antibacterial application. |
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Pseudomonas aeruginosa PAO 1 in vitro timekill kinetics using single phages and phage formulationsmodulating death, adaptation, and resistancePseudomonas aeruginosaBacteriophagestimekillMotilityResistanceScience & TechnologyPseudomonas aeruginosa is responsible for nosocomial and chronic infections in healthcare settings. The major challenge in treating P. aeruginosa-related diseases is its remarkable capacity for antibiotic resistance development. Bacteriophage (phage) therapy is regarded as a possible alternative that has, for years, attracted attention for fighting multidrug-resistant infections. In this work, we characterized five phages showing different lytic spectrums towards clinical isolates. Two of these phages were isolated from the Russian Microgen Sextaphage formulation and belong to the Phikmvviruses, while three Pbunaviruses were isolated from sewage. Different phage formulations for the treatment of P. aeruginosa PAO1 resulted in diversified timekill outcomes. The best result was obtained with a formulation with all phages, prompting a lower frequency of resistant variants and considerable alterations in cell motility, resulting in a loss of 73.7% in swimming motility and a 79% change in swarming motility. These alterations diminished the virulence of the phage-resisting phenotypes but promoted their growth since most became insensitive to a single or even all phages. However, not all combinations drove to enhanced cell killings due to the competition and loss of receptors. This study highlights that more caution is needed when developing cocktail formulations to maximize phage therapy efficacy. Selecting phages for formulations should consider the emergence of phage-resistant bacteria and whether the formulations are intended for short-term or extended antibacterial application.This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2020 unit. S.S. acknowledges funding by FCT through the individual scientific employment program contract (2020.03171.CEECIND).info:eu-repo/semantics/publishedVersionMDPIUniversidade do MinhoPinto, AnaFaustino, AlbertaPastrana, Lorenzo M.Bañobre-López, ManuelSillankorva, Sanna2021-07-192021-07-19T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/73778engPinto, Ana; Faustino, Alberta; Pastrana, Lorenzo M.; Bañobre-López, Manuel; Sillankorva, Sanna, Pseudomonas aeruginosa PAO 1 in vitro timekill kinetics using single phages and phage formulationsmodulating death, adaptation, and resistance. Antibiotics, 10(7), 877, 20212079-63822079-638210.3390/antibiotics10070877https://www.mdpi.com/2079-6382/10/7/877info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:37:53ZPortal AgregadorONG |
dc.title.none.fl_str_mv |
Pseudomonas aeruginosa PAO 1 in vitro timekill kinetics using single phages and phage formulationsmodulating death, adaptation, and resistance |
title |
Pseudomonas aeruginosa PAO 1 in vitro timekill kinetics using single phages and phage formulationsmodulating death, adaptation, and resistance |
spellingShingle |
Pseudomonas aeruginosa PAO 1 in vitro timekill kinetics using single phages and phage formulationsmodulating death, adaptation, and resistance Pinto, Ana Pseudomonas aeruginosa Bacteriophages timekill Motility Resistance Science & Technology |
title_short |
Pseudomonas aeruginosa PAO 1 in vitro timekill kinetics using single phages and phage formulationsmodulating death, adaptation, and resistance |
title_full |
Pseudomonas aeruginosa PAO 1 in vitro timekill kinetics using single phages and phage formulationsmodulating death, adaptation, and resistance |
title_fullStr |
Pseudomonas aeruginosa PAO 1 in vitro timekill kinetics using single phages and phage formulationsmodulating death, adaptation, and resistance |
title_full_unstemmed |
Pseudomonas aeruginosa PAO 1 in vitro timekill kinetics using single phages and phage formulationsmodulating death, adaptation, and resistance |
title_sort |
Pseudomonas aeruginosa PAO 1 in vitro timekill kinetics using single phages and phage formulationsmodulating death, adaptation, and resistance |
author |
Pinto, Ana |
author_facet |
Pinto, Ana Faustino, Alberta Pastrana, Lorenzo M. Bañobre-López, Manuel Sillankorva, Sanna |
author_role |
author |
author2 |
Faustino, Alberta Pastrana, Lorenzo M. Bañobre-López, Manuel Sillankorva, Sanna |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Pinto, Ana Faustino, Alberta Pastrana, Lorenzo M. Bañobre-López, Manuel Sillankorva, Sanna |
dc.subject.por.fl_str_mv |
Pseudomonas aeruginosa Bacteriophages timekill Motility Resistance Science & Technology |
topic |
Pseudomonas aeruginosa Bacteriophages timekill Motility Resistance Science & Technology |
description |
Pseudomonas aeruginosa is responsible for nosocomial and chronic infections in healthcare settings. The major challenge in treating P. aeruginosa-related diseases is its remarkable capacity for antibiotic resistance development. Bacteriophage (phage) therapy is regarded as a possible alternative that has, for years, attracted attention for fighting multidrug-resistant infections. In this work, we characterized five phages showing different lytic spectrums towards clinical isolates. Two of these phages were isolated from the Russian Microgen Sextaphage formulation and belong to the Phikmvviruses, while three Pbunaviruses were isolated from sewage. Different phage formulations for the treatment of P. aeruginosa PAO1 resulted in diversified timekill outcomes. The best result was obtained with a formulation with all phages, prompting a lower frequency of resistant variants and considerable alterations in cell motility, resulting in a loss of 73.7% in swimming motility and a 79% change in swarming motility. These alterations diminished the virulence of the phage-resisting phenotypes but promoted their growth since most became insensitive to a single or even all phages. However, not all combinations drove to enhanced cell killings due to the competition and loss of receptors. This study highlights that more caution is needed when developing cocktail formulations to maximize phage therapy efficacy. Selecting phages for formulations should consider the emergence of phage-resistant bacteria and whether the formulations are intended for short-term or extended antibacterial application. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-07-19 2021-07-19T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/73778 |
url |
http://hdl.handle.net/1822/73778 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pinto, Ana; Faustino, Alberta; Pastrana, Lorenzo M.; Bañobre-López, Manuel; Sillankorva, Sanna, Pseudomonas aeruginosa PAO 1 in vitro timekill kinetics using single phages and phage formulationsmodulating death, adaptation, and resistance. Antibiotics, 10(7), 877, 2021 2079-6382 2079-6382 10.3390/antibiotics10070877 https://www.mdpi.com/2079-6382/10/7/877 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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