Analysis of pro-apoptotic protein trafficking to and from mitochondria

Detalhes bibliográficos
Autor(a) principal: Vega-Naredo, Ignacio
Data de Publicação: 2015
Outros Autores: Cunha-Oliveira, Teresa, Serafim, Teresa L., Sardão, Vilma A., Oliveira, Paulo J.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10316/41110
https://doi.org/10.1007/978-1-4939-1875-1_14
Resumo: Mitochondria play a key role in cell death and its regulation. The permeabilization of the outer mitochondrial membrane which is mainly controlled by proteins of the BCL-2 family, is a key event that can be directly induced by p53 and results in the release of pro-apoptotic factors to the cytosol, such as cytochrome c, second mitochondria derived activator of caspases/direct inhibitor-of-apoptosis (IAP) binding protein with low pI (SMAC/Diablo), Omi serine protease (Omi/HtrA2), apoptosis inducing factor (AIF), or endonuclease G (Endo-G). Hence, the determination of subcellular localization of these proteins is extremely important to predict cell fate and elucidate the specific mechanism of apoptosis. Here we describe the procedures that can be used to study the subcellular location of different pro-apoptotic proteins to be used in basic cell biology and toxicology studies.
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spelling Analysis of pro-apoptotic protein trafficking to and from mitochondriaAnimalsApoptosis Regulatory ProteinsCell FractionationCell LineCell NucleusCytosolElectrophoresis, Polyacrylamide GelFluorescent DyesHumansImmunoblottingImmunohistochemistryImmunoprecipitationMiceMitochondriaProtein TransportRatsTissue FixationMitochondria play a key role in cell death and its regulation. The permeabilization of the outer mitochondrial membrane which is mainly controlled by proteins of the BCL-2 family, is a key event that can be directly induced by p53 and results in the release of pro-apoptotic factors to the cytosol, such as cytochrome c, second mitochondria derived activator of caspases/direct inhibitor-of-apoptosis (IAP) binding protein with low pI (SMAC/Diablo), Omi serine protease (Omi/HtrA2), apoptosis inducing factor (AIF), or endonuclease G (Endo-G). Hence, the determination of subcellular localization of these proteins is extremely important to predict cell fate and elucidate the specific mechanism of apoptosis. Here we describe the procedures that can be used to study the subcellular location of different pro-apoptotic proteins to be used in basic cell biology and toxicology studies.2015info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/41110http://hdl.handle.net/10316/41110https://doi.org/10.1007/978-1-4939-1875-1_14https://doi.org/10.1007/978-1-4939-1875-1_14engVega-Naredo, IgnacioCunha-Oliveira, TeresaSerafim, Teresa L.Sardão, Vilma A.Oliveira, Paulo J.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2021-10-26T12:38:58Zoai:estudogeral.uc.pt:10316/41110Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:53:37.632633Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Analysis of pro-apoptotic protein trafficking to and from mitochondria
title Analysis of pro-apoptotic protein trafficking to and from mitochondria
spellingShingle Analysis of pro-apoptotic protein trafficking to and from mitochondria
Vega-Naredo, Ignacio
Animals
Apoptosis Regulatory Proteins
Cell Fractionation
Cell Line
Cell Nucleus
Cytosol
Electrophoresis, Polyacrylamide Gel
Fluorescent Dyes
Humans
Immunoblotting
Immunohistochemistry
Immunoprecipitation
Mice
Mitochondria
Protein Transport
Rats
Tissue Fixation
title_short Analysis of pro-apoptotic protein trafficking to and from mitochondria
title_full Analysis of pro-apoptotic protein trafficking to and from mitochondria
title_fullStr Analysis of pro-apoptotic protein trafficking to and from mitochondria
title_full_unstemmed Analysis of pro-apoptotic protein trafficking to and from mitochondria
title_sort Analysis of pro-apoptotic protein trafficking to and from mitochondria
author Vega-Naredo, Ignacio
author_facet Vega-Naredo, Ignacio
Cunha-Oliveira, Teresa
Serafim, Teresa L.
Sardão, Vilma A.
Oliveira, Paulo J.
author_role author
author2 Cunha-Oliveira, Teresa
Serafim, Teresa L.
Sardão, Vilma A.
Oliveira, Paulo J.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Vega-Naredo, Ignacio
Cunha-Oliveira, Teresa
Serafim, Teresa L.
Sardão, Vilma A.
Oliveira, Paulo J.
dc.subject.por.fl_str_mv Animals
Apoptosis Regulatory Proteins
Cell Fractionation
Cell Line
Cell Nucleus
Cytosol
Electrophoresis, Polyacrylamide Gel
Fluorescent Dyes
Humans
Immunoblotting
Immunohistochemistry
Immunoprecipitation
Mice
Mitochondria
Protein Transport
Rats
Tissue Fixation
topic Animals
Apoptosis Regulatory Proteins
Cell Fractionation
Cell Line
Cell Nucleus
Cytosol
Electrophoresis, Polyacrylamide Gel
Fluorescent Dyes
Humans
Immunoblotting
Immunohistochemistry
Immunoprecipitation
Mice
Mitochondria
Protein Transport
Rats
Tissue Fixation
description Mitochondria play a key role in cell death and its regulation. The permeabilization of the outer mitochondrial membrane which is mainly controlled by proteins of the BCL-2 family, is a key event that can be directly induced by p53 and results in the release of pro-apoptotic factors to the cytosol, such as cytochrome c, second mitochondria derived activator of caspases/direct inhibitor-of-apoptosis (IAP) binding protein with low pI (SMAC/Diablo), Omi serine protease (Omi/HtrA2), apoptosis inducing factor (AIF), or endonuclease G (Endo-G). Hence, the determination of subcellular localization of these proteins is extremely important to predict cell fate and elucidate the specific mechanism of apoptosis. Here we describe the procedures that can be used to study the subcellular location of different pro-apoptotic proteins to be used in basic cell biology and toxicology studies.
publishDate 2015
dc.date.none.fl_str_mv 2015
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10316/41110
http://hdl.handle.net/10316/41110
https://doi.org/10.1007/978-1-4939-1875-1_14
https://doi.org/10.1007/978-1-4939-1875-1_14
url http://hdl.handle.net/10316/41110
https://doi.org/10.1007/978-1-4939-1875-1_14
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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