Oral mucositis caused by Candida glabrata biofilms: failure of the concomitant use of fluconazole and ascorbic acid
Autor(a) principal: | |
---|---|
Data de Publicação: | 2017 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/44996 |
Resumo: | Objectives: Candida glabrata is becoming one of the most prevalent pathogenic yeasts in cases of oral diseases. Mucositis is an recurrent oral infection in immunocompromised patients, and the actual guidelines recommend the use of fluconazole (Flu) for many cases. However, the azole resistance by C. glabrata is renowned, causing a reduced therapeutic response, especially when it occurs in biofilms. In this study, we performed an in vitro evaluation of an alternative pharmacotherapy for C. glabrata biofilm infections, combining ascorbic acid (AA) with Flu. AA is recognized for degrading -glucans, an important compound of the biofilm matrices, which prevent drug diffusion. Materials and Methods: Thus, routine clinical 30 or 40mg/l doses of Flu were applied to C. glabrata biofilms simultaneously with 200 or 300mg/l of AA. Results: The results showed that this combination effectively promoted the degradation of the biofilm network, but unfortunately, also stimulated the growth of the yeasts population due to release of several glucose monomers during -glucans hydrolysis. Conclusions: As a result, it was proven that, contrary to what happens in treatment of bacterial infection, AA should not be used together with Flu in the treatment of oral mucositis caused by Candida. |
id |
RCAP_68d32595252a8d7068ea87cf1433a256 |
---|---|
oai_identifier_str |
oai:repositorium.sdum.uminho.pt:1822/44996 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Oral mucositis caused by Candida glabrata biofilms: failure of the concomitant use of fluconazole and ascorbic acidCandidaOral mucositisFluconazoleAscorbic acidCandida glabrataAntifungal resistanceBiofilmsbiofilm matrixinfectionresistanceScience & TechnologyObjectives: Candida glabrata is becoming one of the most prevalent pathogenic yeasts in cases of oral diseases. Mucositis is an recurrent oral infection in immunocompromised patients, and the actual guidelines recommend the use of fluconazole (Flu) for many cases. However, the azole resistance by C. glabrata is renowned, causing a reduced therapeutic response, especially when it occurs in biofilms. In this study, we performed an in vitro evaluation of an alternative pharmacotherapy for C. glabrata biofilm infections, combining ascorbic acid (AA) with Flu. AA is recognized for degrading -glucans, an important compound of the biofilm matrices, which prevent drug diffusion. Materials and Methods: Thus, routine clinical 30 or 40mg/l doses of Flu were applied to C. glabrata biofilms simultaneously with 200 or 300mg/l of AA. Results: The results showed that this combination effectively promoted the degradation of the biofilm network, but unfortunately, also stimulated the growth of the yeasts population due to release of several glucose monomers during -glucans hydrolysis. Conclusions: As a result, it was proven that, contrary to what happens in treatment of bacterial infection, AA should not be used together with Flu in the treatment of oral mucositis caused by Candida.The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was supported by the Programa Operacional, Fatores de competitividade – COMPETE and by national funds through FCT – Fundação para a Ciência e a Tecnologia on the scope of the projects FCT PTDC/SAU-MIC/119069/2010, RECI/EBBEBI/0179/2012 and PEst-OE/EQB/LA0023/2013 and Célia F. Rodrigues’ SFRH/BD/93078/2013 PhD grant.SAGEUniversidade do MinhoRodrigues, Célia F.Henriques, Mariana2017-032017-03-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/44996engRodrigues, Célia F.; Henriques, Mariana, Oral mucositis caused by Candida glabrata biofilms: failure of the concomitant use of fluconazole and ascorbic acid. Therapeutic Advances in Infectious Disease, 4(1), 10-17, 20172049-93612049-937X10.1177/2049936116684477http://journals.sagepub.com/home/taiinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:45:02Zoai:repositorium.sdum.uminho.pt:1822/44996Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:42:50.138103Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Oral mucositis caused by Candida glabrata biofilms: failure of the concomitant use of fluconazole and ascorbic acid |
title |
Oral mucositis caused by Candida glabrata biofilms: failure of the concomitant use of fluconazole and ascorbic acid |
spellingShingle |
Oral mucositis caused by Candida glabrata biofilms: failure of the concomitant use of fluconazole and ascorbic acid Rodrigues, Célia F. Candida Oral mucositis Fluconazole Ascorbic acid Candida glabrata Antifungal resistance Biofilms biofilm matrix infection resistance Science & Technology |
title_short |
Oral mucositis caused by Candida glabrata biofilms: failure of the concomitant use of fluconazole and ascorbic acid |
title_full |
Oral mucositis caused by Candida glabrata biofilms: failure of the concomitant use of fluconazole and ascorbic acid |
title_fullStr |
Oral mucositis caused by Candida glabrata biofilms: failure of the concomitant use of fluconazole and ascorbic acid |
title_full_unstemmed |
Oral mucositis caused by Candida glabrata biofilms: failure of the concomitant use of fluconazole and ascorbic acid |
title_sort |
Oral mucositis caused by Candida glabrata biofilms: failure of the concomitant use of fluconazole and ascorbic acid |
author |
Rodrigues, Célia F. |
author_facet |
Rodrigues, Célia F. Henriques, Mariana |
author_role |
author |
author2 |
Henriques, Mariana |
author2_role |
author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Rodrigues, Célia F. Henriques, Mariana |
dc.subject.por.fl_str_mv |
Candida Oral mucositis Fluconazole Ascorbic acid Candida glabrata Antifungal resistance Biofilms biofilm matrix infection resistance Science & Technology |
topic |
Candida Oral mucositis Fluconazole Ascorbic acid Candida glabrata Antifungal resistance Biofilms biofilm matrix infection resistance Science & Technology |
description |
Objectives: Candida glabrata is becoming one of the most prevalent pathogenic yeasts in cases of oral diseases. Mucositis is an recurrent oral infection in immunocompromised patients, and the actual guidelines recommend the use of fluconazole (Flu) for many cases. However, the azole resistance by C. glabrata is renowned, causing a reduced therapeutic response, especially when it occurs in biofilms. In this study, we performed an in vitro evaluation of an alternative pharmacotherapy for C. glabrata biofilm infections, combining ascorbic acid (AA) with Flu. AA is recognized for degrading -glucans, an important compound of the biofilm matrices, which prevent drug diffusion. Materials and Methods: Thus, routine clinical 30 or 40mg/l doses of Flu were applied to C. glabrata biofilms simultaneously with 200 or 300mg/l of AA. Results: The results showed that this combination effectively promoted the degradation of the biofilm network, but unfortunately, also stimulated the growth of the yeasts population due to release of several glucose monomers during -glucans hydrolysis. Conclusions: As a result, it was proven that, contrary to what happens in treatment of bacterial infection, AA should not be used together with Flu in the treatment of oral mucositis caused by Candida. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-03 2017-03-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/44996 |
url |
http://hdl.handle.net/1822/44996 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Rodrigues, Célia F.; Henriques, Mariana, Oral mucositis caused by Candida glabrata biofilms: failure of the concomitant use of fluconazole and ascorbic acid. Therapeutic Advances in Infectious Disease, 4(1), 10-17, 2017 2049-9361 2049-937X 10.1177/2049936116684477 http://journals.sagepub.com/home/tai |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
SAGE |
publisher.none.fl_str_mv |
SAGE |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799132983062429696 |