Generation and characterization of a human iPS cell line from a patient-related control to study disease mechanisms associated with DAND5 p.R152H alteration

Detalhes bibliográficos
Autor(a) principal: Pars, Selin
Data de Publicação: 2018
Outros Autores: Cristo, Fernando, Inácio, José M., Rosas, Graça, Carreira, Isabel Marques, Melo, Joana Barbosa, Mendes, Patrícia, Martins, Duarte Saraiva, Almeida, Luís Pereira de, Maio, José, Anjos, Rui, Belo, José A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10316/108062
https://doi.org/10.1016/j.scr.2018.04.015
Resumo: A DAND5-control human iPSC line was generated from the urinary cells of a phenotypically normal donor. Exfoliated renal epithelial (RE) cells were collected and reprogrammed into iPSCs using Sendai virus reprogramming system. The pluripotency, in vitro differentiation potential, karyotype stability, and the transgene-free status of generated iPSC line were analyzed and confirmed. This cell line can be exploited as a control iPSC line to better understand the mechanisms involved in DAND5-associated cardiac disease.
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spelling Generation and characterization of a human iPS cell line from a patient-related control to study disease mechanisms associated with DAND5 p.R152H alterationCell LineHumansMaleCellular Reprogramming TechniquesHeart DiseasesInduced Pluripotent Stem CellsIntercellular Signaling Peptides and ProteinsA DAND5-control human iPSC line was generated from the urinary cells of a phenotypically normal donor. Exfoliated renal epithelial (RE) cells were collected and reprogrammed into iPSCs using Sendai virus reprogramming system. The pluripotency, in vitro differentiation potential, karyotype stability, and the transgene-free status of generated iPSC line were analyzed and confirmed. This cell line can be exploited as a control iPSC line to better understand the mechanisms involved in DAND5-associated cardiac disease.Elsevier2018-05info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/108062http://hdl.handle.net/10316/108062https://doi.org/10.1016/j.scr.2018.04.015eng18735061Pars, SelinCristo, FernandoInácio, José M.Rosas, GraçaCarreira, Isabel MarquesMelo, Joana BarbosaMendes, PatríciaMartins, Duarte SaraivaAlmeida, Luís Pereira deMaio, JoséAnjos, RuiBelo, José A.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-08-09T08:34:52Zoai:estudogeral.uc.pt:10316/108062Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:24:19.914531Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Generation and characterization of a human iPS cell line from a patient-related control to study disease mechanisms associated with DAND5 p.R152H alteration
title Generation and characterization of a human iPS cell line from a patient-related control to study disease mechanisms associated with DAND5 p.R152H alteration
spellingShingle Generation and characterization of a human iPS cell line from a patient-related control to study disease mechanisms associated with DAND5 p.R152H alteration
Pars, Selin
Cell Line
Humans
Male
Cellular Reprogramming Techniques
Heart Diseases
Induced Pluripotent Stem Cells
Intercellular Signaling Peptides and Proteins
title_short Generation and characterization of a human iPS cell line from a patient-related control to study disease mechanisms associated with DAND5 p.R152H alteration
title_full Generation and characterization of a human iPS cell line from a patient-related control to study disease mechanisms associated with DAND5 p.R152H alteration
title_fullStr Generation and characterization of a human iPS cell line from a patient-related control to study disease mechanisms associated with DAND5 p.R152H alteration
title_full_unstemmed Generation and characterization of a human iPS cell line from a patient-related control to study disease mechanisms associated with DAND5 p.R152H alteration
title_sort Generation and characterization of a human iPS cell line from a patient-related control to study disease mechanisms associated with DAND5 p.R152H alteration
author Pars, Selin
author_facet Pars, Selin
Cristo, Fernando
Inácio, José M.
Rosas, Graça
Carreira, Isabel Marques
Melo, Joana Barbosa
Mendes, Patrícia
Martins, Duarte Saraiva
Almeida, Luís Pereira de
Maio, José
Anjos, Rui
Belo, José A.
author_role author
author2 Cristo, Fernando
Inácio, José M.
Rosas, Graça
Carreira, Isabel Marques
Melo, Joana Barbosa
Mendes, Patrícia
Martins, Duarte Saraiva
Almeida, Luís Pereira de
Maio, José
Anjos, Rui
Belo, José A.
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Pars, Selin
Cristo, Fernando
Inácio, José M.
Rosas, Graça
Carreira, Isabel Marques
Melo, Joana Barbosa
Mendes, Patrícia
Martins, Duarte Saraiva
Almeida, Luís Pereira de
Maio, José
Anjos, Rui
Belo, José A.
dc.subject.por.fl_str_mv Cell Line
Humans
Male
Cellular Reprogramming Techniques
Heart Diseases
Induced Pluripotent Stem Cells
Intercellular Signaling Peptides and Proteins
topic Cell Line
Humans
Male
Cellular Reprogramming Techniques
Heart Diseases
Induced Pluripotent Stem Cells
Intercellular Signaling Peptides and Proteins
description A DAND5-control human iPSC line was generated from the urinary cells of a phenotypically normal donor. Exfoliated renal epithelial (RE) cells were collected and reprogrammed into iPSCs using Sendai virus reprogramming system. The pluripotency, in vitro differentiation potential, karyotype stability, and the transgene-free status of generated iPSC line were analyzed and confirmed. This cell line can be exploited as a control iPSC line to better understand the mechanisms involved in DAND5-associated cardiac disease.
publishDate 2018
dc.date.none.fl_str_mv 2018-05
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10316/108062
http://hdl.handle.net/10316/108062
https://doi.org/10.1016/j.scr.2018.04.015
url http://hdl.handle.net/10316/108062
https://doi.org/10.1016/j.scr.2018.04.015
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 18735061
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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