Slaughterhouses Fungal Burden Assessment: A Contribution for the Pursuit of a Better Assessment Strategy

Bibliographic Details
Main Author: Viegas, Carla
Publication Date: 2016
Other Authors: Faria, Tiago, dos Santos, Mateus, Carolino, Elisabete, Sabino, Raquel, Quintal Gomes, Anita, Viegas, Susana
Format: Article
Language: eng
Source: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Download full: http://hdl.handle.net/10400.18/4277
Summary: Free PMC Article: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4808960/
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spelling Slaughterhouses Fungal Burden Assessment: A Contribution for the Pursuit of a Better Assessment StrategyFungal BurdenAssessment StrategySlaughterhousesInfecções Sistémicas e ZoonosesFree PMC Article: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4808960/In slaughterhouses, the biological risk is present not only from the direct or indirect contact with animal matter, but also from the exposure to bioaerosols. Fungal contamination was already reported from the floors and walls of slaughterhouses. This study intends to assess fungal contamination by cultural and molecular methods in poultry, swine/bovine and large animal slaughterhouses. Air samples were collected through an impaction method, while surface samples were collected by the swabbing method and subjected to further macro- and micro-scopic observations. In addition, we collected air samples using the impinger method in order to perform real-time quantitative PCR (qPCR) amplification of genes from specific fungal species, namely A. flavus, A. fumigatus and A. ochraceus complexes. Poultry and swine/bovine slaughterhouses presented each two sampling sites that surpass the guideline of 150 CFU/m3. Scopulariopsis candida was the most frequently isolated (59.5%) in poultry slaughterhouse air; Cladosporium sp. (45.7%) in the swine/bovine slaughterhouse; and Penicillium sp. (80.8%) in the large animal slaughterhouse. Molecular tools successfully amplified DNA from the A. fumigatus complex in six sampling sites where the presence of this fungal species was not identified by conventional methods. This study besides suggesting the indicators that are representative of harmful fungal contamination, also indicates a strategy as a protocol to ensure a proper characterization of fungal occupational exposure.This study was supported by the Environment & Health Research Group from Lisbon School of Health Technology and Associação Para o Desenvolvimento de Conhecimento e Inovação (POLITEC & ID).MDPIRepositório Científico do Instituto Nacional de SaúdeViegas, CarlaFaria, Tiagodos Santos, MateusCarolino, ElisabeteSabino, RaquelQuintal Gomes, AnitaViegas, Susana2017-02-17T16:05:24Z2016-03-082016-03-08T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/4277engInt J Environ Res Public Health. 2016 Mar 8;13(3). pii: E297. doi: 10.3390/ijerph130302971660-460110.3390/ijerph13030297info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:40:22Zoai:repositorio.insa.pt:10400.18/4277Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:39:16.558622Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Slaughterhouses Fungal Burden Assessment: A Contribution for the Pursuit of a Better Assessment Strategy
title Slaughterhouses Fungal Burden Assessment: A Contribution for the Pursuit of a Better Assessment Strategy
spellingShingle Slaughterhouses Fungal Burden Assessment: A Contribution for the Pursuit of a Better Assessment Strategy
Viegas, Carla
Fungal Burden
Assessment Strategy
Slaughterhouses
Infecções Sistémicas e Zoonoses
title_short Slaughterhouses Fungal Burden Assessment: A Contribution for the Pursuit of a Better Assessment Strategy
title_full Slaughterhouses Fungal Burden Assessment: A Contribution for the Pursuit of a Better Assessment Strategy
title_fullStr Slaughterhouses Fungal Burden Assessment: A Contribution for the Pursuit of a Better Assessment Strategy
title_full_unstemmed Slaughterhouses Fungal Burden Assessment: A Contribution for the Pursuit of a Better Assessment Strategy
title_sort Slaughterhouses Fungal Burden Assessment: A Contribution for the Pursuit of a Better Assessment Strategy
author Viegas, Carla
author_facet Viegas, Carla
Faria, Tiago
dos Santos, Mateus
Carolino, Elisabete
Sabino, Raquel
Quintal Gomes, Anita
Viegas, Susana
author_role author
author2 Faria, Tiago
dos Santos, Mateus
Carolino, Elisabete
Sabino, Raquel
Quintal Gomes, Anita
Viegas, Susana
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Nacional de Saúde
dc.contributor.author.fl_str_mv Viegas, Carla
Faria, Tiago
dos Santos, Mateus
Carolino, Elisabete
Sabino, Raquel
Quintal Gomes, Anita
Viegas, Susana
dc.subject.por.fl_str_mv Fungal Burden
Assessment Strategy
Slaughterhouses
Infecções Sistémicas e Zoonoses
topic Fungal Burden
Assessment Strategy
Slaughterhouses
Infecções Sistémicas e Zoonoses
description Free PMC Article: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4808960/
publishDate 2016
dc.date.none.fl_str_mv 2016-03-08
2016-03-08T00:00:00Z
2017-02-17T16:05:24Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.18/4277
url http://hdl.handle.net/10400.18/4277
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Int J Environ Res Public Health. 2016 Mar 8;13(3). pii: E297. doi: 10.3390/ijerph13030297
1660-4601
10.3390/ijerph13030297
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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