Understanding how capping of actin filaments prevents epithelium to mesenchymal like transitions in genetically defined epithelial tissues

Detalhes bibliográficos
Autor(a) principal: Rebelo, Sofia Raquel Paulo
Data de Publicação: 2008
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/1405
Resumo: Tese de mestrado, Biologia (Biologia Evolutiva e do Desenvolvimento), 2007, Universidade de Lisboa, Faculdade de Ciências
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spelling Understanding how capping of actin filaments prevents epithelium to mesenchymal like transitions in genetically defined epithelial tissuesBiologia celularApoptoseCélulas epiteliaisTeses de mestradoTese de mestrado, Biologia (Biologia Evolutiva e do Desenvolvimento), 2007, Universidade de Lisboa, Faculdade de CiênciasThe actin cytoskeleton has a central role in controlling cell shape and mobility. In epithelia, a circumferential band of actin filaments provides the structural support for cell-cell junctions. When the strength of the epithelium is compromised, cells may undergo epithelium to mesenchymal transition, escape size-control mechanisms, evade cell death and finally acquire the ability to migrate. These features recapitulate all of the hallmarks that characterize cancer malignancy. Interestingly, clones of cells mutant for either subunits of the capping protein ab heterodimer (CP), induced in a heterozygous wild-type background, are extruded from the wing blade epithelium and die. However, depleting the subunit (cpb) by RNA interference (RNAi) in the whole blade leads to a different outcome: epithelium polarity is strongly affected, few apoptotic cells can be observed, while many seem to overproliferate. This differential behavior is unlikely to be due to a dosage effect since RNAi induced-cpb depletion in restricted wing blade domains also induces cell extrusion and death. This suggests that CP prevents tumorigenesis of wing blade cells. However, when CP mutant cells are adjacent to wildtype wing blade neighboring cells, the latest eliminate mutant cells by a process of cell competition. The role of CP in preventing tumorigenesis might be related to its major function, preventing excessive actin polimerization, or to additional functions, such as the maintenance of epithelial cell polarity. The tumor suppressor function of CP appears to be tissue specific since the above cell behavior is only observed in restricted epithelia. This suggests that each epithelium has specific cytoskeleton and/or junctional properties, making cells sensitive or not to mutations that cause abnormal tumor growth. Altogether the presented data highlight the crucial impact of tissue context for the activation of a tumoral processResumo alargado em português disponível no documentoJanody, FlorenceRodrigues, Maria GabrielaRepositório da Universidade de LisboaRebelo, Sofia Raquel Paulo2010-07-27T08:58:04Z20082008-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdftext/xmlhttp://hdl.handle.net/10451/1405enghttp://catalogo.ul.pt/F/?func=item-global&doc_library=ULB01&type=03&doc_number=000568794info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T15:40:54Zoai:repositorio.ul.pt:10451/1405Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:28:03.265890Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Understanding how capping of actin filaments prevents epithelium to mesenchymal like transitions in genetically defined epithelial tissues
title Understanding how capping of actin filaments prevents epithelium to mesenchymal like transitions in genetically defined epithelial tissues
spellingShingle Understanding how capping of actin filaments prevents epithelium to mesenchymal like transitions in genetically defined epithelial tissues
Rebelo, Sofia Raquel Paulo
Biologia celular
Apoptose
Células epiteliais
Teses de mestrado
title_short Understanding how capping of actin filaments prevents epithelium to mesenchymal like transitions in genetically defined epithelial tissues
title_full Understanding how capping of actin filaments prevents epithelium to mesenchymal like transitions in genetically defined epithelial tissues
title_fullStr Understanding how capping of actin filaments prevents epithelium to mesenchymal like transitions in genetically defined epithelial tissues
title_full_unstemmed Understanding how capping of actin filaments prevents epithelium to mesenchymal like transitions in genetically defined epithelial tissues
title_sort Understanding how capping of actin filaments prevents epithelium to mesenchymal like transitions in genetically defined epithelial tissues
author Rebelo, Sofia Raquel Paulo
author_facet Rebelo, Sofia Raquel Paulo
author_role author
dc.contributor.none.fl_str_mv Janody, Florence
Rodrigues, Maria Gabriela
Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Rebelo, Sofia Raquel Paulo
dc.subject.por.fl_str_mv Biologia celular
Apoptose
Células epiteliais
Teses de mestrado
topic Biologia celular
Apoptose
Células epiteliais
Teses de mestrado
description Tese de mestrado, Biologia (Biologia Evolutiva e do Desenvolvimento), 2007, Universidade de Lisboa, Faculdade de Ciências
publishDate 2008
dc.date.none.fl_str_mv 2008
2008-01-01T00:00:00Z
2010-07-27T08:58:04Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/1405
url http://hdl.handle.net/10451/1405
dc.language.iso.fl_str_mv eng
language eng
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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