Characterization of the wood mycobiome of Vitis vinifera in a vineyard affected by Esca. Spatial distribution of fungal communities and their putative relation with leaf symptoms

Detalhes bibliográficos
Autor(a) principal: Del Frari, Giovanni
Data de Publicação: 2019
Outros Autores: Gobbi, Alex, Aggerbeck, Marie Ronne, Oliveira, Helena, Hansen, Lars Hestbjerg, Ferreira, Ricardo Boavida
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/18220
Resumo: Original Research
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spelling Characterization of the wood mycobiome of Vitis vinifera in a vineyard affected by Esca. Spatial distribution of fungal communities and their putative relation with leaf symptomsVitis viniferamicrobial ecologymatabarcodingmycobiomeEsca diseasegrapevine trunk diseasesOriginal ResearchEsca is a disease complex belonging to the grapevine trunk diseases cluster. It comprises five syndromes, three main fungal pathogenic agents and several symptoms, both internal (i.e., affecting woody tissue) and external (e.g., affecting leaves and bunches). The etiology and epidemiology of this disease complex remain, in part, unclear. Some of the points that are still under discussion concern the sudden rise in disease incidence, the simultaneous presence of multiple wood pathogens in affected grapevines, the causal agents and the discontinuity in time of leaf symptoms manifestation. The standard approach to the study of esca has been mostly through culture-dependent studies, yet, leaving many questions unanswered. In this study, we used Illumina R next-generation amplicon sequencing to investigate the mycobiome of grapevines wood in a vineyard with history of esca. We characterized the wood mycobiome composition, investigated the spatial dynamics of the fungal communities in different areas of the stem and in canes, and assessed the putative link between mycobiome and leaf symptoms. An unprecedented diversity of fungi is presented (289 taxa), including five genera reported for the first time in association with grapevines wood (Debaryomyces, Trematosphaeria, Biatriospora, Lopadostoma, and Malassezia) and numerous hitherto unreported species. Esca-associated fungi Phaeomoniella chlamydospora and Fomitiporia sp. dominate the fungal community, and numerous other fungi associated with wood syndromes are also encountered (e.g., Eutypa spp., Inonotus hispidus). The spatial analysis revealed differences in diversity, evenness and taxa abundances, the unique presence of certain fungi in specific areas of the plants, and tissue specificity. Lastly, the mycobiome composition of the woody tissue in proximity to leaves manifesting ‘tiger stripes’ symptoms of esca, as well as in leaf-symptomatic canes, was highly similar to that of plants not exhibiting any leaf symptomatology. This observation supports the current understanding that leaf symptoms are not directly linked with the fungal communities in the wood. This work builds to the understanding of the microbial ecology of the grapevines wood, offering insights and a critical view on the current knowledge of the etiology of escaMichele Perazzoli, Fondazione Edmundo Mach, ItalyRepositório da Universidade de LisboaDel Frari, GiovanniGobbi, AlexAggerbeck, Marie RonneOliveira, HelenaHansen, Lars HestbjergFerreira, Ricardo Boavida2019-08-08T13:09:42Z20192019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/18220engDel Frari G, Gobbi A, Aggerbeck MR, Oliveira H, Hansen LH and Ferreira RB (2019) Characterization of the Wood Mycobiome of Vitis vinifera in a Vineyard Affected by Esca. Spatial Distribution of Fungal Communities and Their Putative Relation With Leaf Symptoms. Front. Plant Sci. 10:91010.3389/fpls.2019.00910info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:47:50Zoai:www.repository.utl.pt:10400.5/18220Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:03:18.578821Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Characterization of the wood mycobiome of Vitis vinifera in a vineyard affected by Esca. Spatial distribution of fungal communities and their putative relation with leaf symptoms
title Characterization of the wood mycobiome of Vitis vinifera in a vineyard affected by Esca. Spatial distribution of fungal communities and their putative relation with leaf symptoms
spellingShingle Characterization of the wood mycobiome of Vitis vinifera in a vineyard affected by Esca. Spatial distribution of fungal communities and their putative relation with leaf symptoms
Del Frari, Giovanni
Vitis vinifera
microbial ecology
matabarcoding
mycobiome
Esca disease
grapevine trunk diseases
title_short Characterization of the wood mycobiome of Vitis vinifera in a vineyard affected by Esca. Spatial distribution of fungal communities and their putative relation with leaf symptoms
title_full Characterization of the wood mycobiome of Vitis vinifera in a vineyard affected by Esca. Spatial distribution of fungal communities and their putative relation with leaf symptoms
title_fullStr Characterization of the wood mycobiome of Vitis vinifera in a vineyard affected by Esca. Spatial distribution of fungal communities and their putative relation with leaf symptoms
title_full_unstemmed Characterization of the wood mycobiome of Vitis vinifera in a vineyard affected by Esca. Spatial distribution of fungal communities and their putative relation with leaf symptoms
title_sort Characterization of the wood mycobiome of Vitis vinifera in a vineyard affected by Esca. Spatial distribution of fungal communities and their putative relation with leaf symptoms
author Del Frari, Giovanni
author_facet Del Frari, Giovanni
Gobbi, Alex
Aggerbeck, Marie Ronne
Oliveira, Helena
Hansen, Lars Hestbjerg
Ferreira, Ricardo Boavida
author_role author
author2 Gobbi, Alex
Aggerbeck, Marie Ronne
Oliveira, Helena
Hansen, Lars Hestbjerg
Ferreira, Ricardo Boavida
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Del Frari, Giovanni
Gobbi, Alex
Aggerbeck, Marie Ronne
Oliveira, Helena
Hansen, Lars Hestbjerg
Ferreira, Ricardo Boavida
dc.subject.por.fl_str_mv Vitis vinifera
microbial ecology
matabarcoding
mycobiome
Esca disease
grapevine trunk diseases
topic Vitis vinifera
microbial ecology
matabarcoding
mycobiome
Esca disease
grapevine trunk diseases
description Original Research
publishDate 2019
dc.date.none.fl_str_mv 2019-08-08T13:09:42Z
2019
2019-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/18220
url http://hdl.handle.net/10400.5/18220
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Del Frari G, Gobbi A, Aggerbeck MR, Oliveira H, Hansen LH and Ferreira RB (2019) Characterization of the Wood Mycobiome of Vitis vinifera in a Vineyard Affected by Esca. Spatial Distribution of Fungal Communities and Their Putative Relation With Leaf Symptoms. Front. Plant Sci. 10:910
10.3389/fpls.2019.00910
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Michele Perazzoli, Fondazione Edmundo Mach, Italy
publisher.none.fl_str_mv Michele Perazzoli, Fondazione Edmundo Mach, Italy
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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