New insights into the cell cycle profile of Paracoccidioides brasiliensis
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/5184 |
Resumo: | The present work focuses on the analysis of cell cycle progression of Paracoccidioides brasiliensis yeast cells under diVerent environmental conditions. We optimized a Xow cytometric technique for cell cycle proWle analysis based on high resolution measurements of nuclear DNA. Exponentially growing cells in poor-deWned or rich-complex nutritional environments showed an increased percentage of daughter cells in accordance with the fungus’ multiple budding and high growth rate. During the stationary growth-phase cell cycle progression in rich-complex medium was characterized by an accumulation of cells with higher DNA content or pseudohyphae-like structures, whereas in poor-deWned medium arrested cells mainly displayed two DNA contents. Furthermore, the fungicide benomyl induced an arrest of the cell cycle with accumulation of cells presenting high and varying DNA contents, consistent with this fungus’ unique pattern of cellular division. Altogether, our Wndings seem to indicate that P. brasiliensis may possess alternative control mechanisms during cell growth to manage multiple budding and its multinucleate nature. |
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New insights into the cell cycle profile of Paracoccidioides brasiliensisParacoccidioides brasiliensisFlow cytometryMultiple buddingCell cycle profileCell cycle arrestScience & TechnologyThe present work focuses on the analysis of cell cycle progression of Paracoccidioides brasiliensis yeast cells under diVerent environmental conditions. We optimized a Xow cytometric technique for cell cycle proWle analysis based on high resolution measurements of nuclear DNA. Exponentially growing cells in poor-deWned or rich-complex nutritional environments showed an increased percentage of daughter cells in accordance with the fungus’ multiple budding and high growth rate. During the stationary growth-phase cell cycle progression in rich-complex medium was characterized by an accumulation of cells with higher DNA content or pseudohyphae-like structures, whereas in poor-deWned medium arrested cells mainly displayed two DNA contents. Furthermore, the fungicide benomyl induced an arrest of the cell cycle with accumulation of cells presenting high and varying DNA contents, consistent with this fungus’ unique pattern of cellular division. Altogether, our Wndings seem to indicate that P. brasiliensis may possess alternative control mechanisms during cell growth to manage multiple budding and its multinucleate nature.Fundação para a Ciência e a Tecnologia (FCT) - Contract SFRH/BD/8655/2002, Grant No. POCTI/ESP/45327/ 2002.ElsevierUniversidade do MinhoAlmeida, A. J.Martins, M.Carmona, J. A.Cano, L. E.Restrepo, A.Leão, CecíliaRodrigues, Fernando José dos Santos20062006-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/5184eng"Fungal Genetics and Biology". ISSN 1087-1845. 43:6 (2006) 401-409.1087-184510.1016/j.fgb.2006.01.01216631397http://www.elsevier.com/wps/find/journaldescription.cws_home/622835/description#descriptioninfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:46:19Zoai:repositorium.sdum.uminho.pt:1822/5184Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:44:18.396492Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
New insights into the cell cycle profile of Paracoccidioides brasiliensis |
title |
New insights into the cell cycle profile of Paracoccidioides brasiliensis |
spellingShingle |
New insights into the cell cycle profile of Paracoccidioides brasiliensis Almeida, A. J. Paracoccidioides brasiliensis Flow cytometry Multiple budding Cell cycle profile Cell cycle arrest Science & Technology |
title_short |
New insights into the cell cycle profile of Paracoccidioides brasiliensis |
title_full |
New insights into the cell cycle profile of Paracoccidioides brasiliensis |
title_fullStr |
New insights into the cell cycle profile of Paracoccidioides brasiliensis |
title_full_unstemmed |
New insights into the cell cycle profile of Paracoccidioides brasiliensis |
title_sort |
New insights into the cell cycle profile of Paracoccidioides brasiliensis |
author |
Almeida, A. J. |
author_facet |
Almeida, A. J. Martins, M. Carmona, J. A. Cano, L. E. Restrepo, A. Leão, Cecília Rodrigues, Fernando José dos Santos |
author_role |
author |
author2 |
Martins, M. Carmona, J. A. Cano, L. E. Restrepo, A. Leão, Cecília Rodrigues, Fernando José dos Santos |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Almeida, A. J. Martins, M. Carmona, J. A. Cano, L. E. Restrepo, A. Leão, Cecília Rodrigues, Fernando José dos Santos |
dc.subject.por.fl_str_mv |
Paracoccidioides brasiliensis Flow cytometry Multiple budding Cell cycle profile Cell cycle arrest Science & Technology |
topic |
Paracoccidioides brasiliensis Flow cytometry Multiple budding Cell cycle profile Cell cycle arrest Science & Technology |
description |
The present work focuses on the analysis of cell cycle progression of Paracoccidioides brasiliensis yeast cells under diVerent environmental conditions. We optimized a Xow cytometric technique for cell cycle proWle analysis based on high resolution measurements of nuclear DNA. Exponentially growing cells in poor-deWned or rich-complex nutritional environments showed an increased percentage of daughter cells in accordance with the fungus’ multiple budding and high growth rate. During the stationary growth-phase cell cycle progression in rich-complex medium was characterized by an accumulation of cells with higher DNA content or pseudohyphae-like structures, whereas in poor-deWned medium arrested cells mainly displayed two DNA contents. Furthermore, the fungicide benomyl induced an arrest of the cell cycle with accumulation of cells presenting high and varying DNA contents, consistent with this fungus’ unique pattern of cellular division. Altogether, our Wndings seem to indicate that P. brasiliensis may possess alternative control mechanisms during cell growth to manage multiple budding and its multinucleate nature. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006 2006-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/5184 |
url |
http://hdl.handle.net/1822/5184 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
"Fungal Genetics and Biology". ISSN 1087-1845. 43:6 (2006) 401-409. 1087-1845 10.1016/j.fgb.2006.01.012 16631397 http://www.elsevier.com/wps/find/journaldescription.cws_home/622835/description#description |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133003710988288 |