Anti-Inflammatory properties of injectable Betamethasone-Loaded Tyramine-Modified Gellan Gum/Silk Fibroin Hydrogels
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/67962 |
Resumo: | Rheumatoid arthritis is a rheumatic disease for which a healing treatment does not presently exist. Silk fibroin has been extensively studied for use in drug delivery systems due to its uniqueness, versatility and strong clinical track record in medicine. However, in general, natural polymeric materials are not mechanically stable enough, and have high rates of biodegradation. Thus, synthetic materials such as gellan gum can be used to produce composite structures with biological signals to promote tissue-specific interactions while providing the desired mechanical properties. In this work, we aimed to produce hydrogels of tyramine-modified gellan gum with silk fibroin (Tyâ GG/SF) via horseradish peroxidase (HRP), with encapsulated betamethasone, to improve the biocompatibility and mechanical properties, and further increase therapeutic efficacy to treat rheumatoid arthritis (RA). The Tyâ GG/SF hydrogels presented a β-sheet secondary structure, with gelation time around 2â 5 min, good resistance to enzymatic degradation, a suitable injectability profile, viscoelastic capacity with a significant solid component and a betamethasone-controlled release profile over time. In vitro studies showed that Tyâ GG/SF hydrogels did not produce a deleterious effect on cellular metabolic activity, morphology or proliferation. Furthermore, Tyâ GG/SF hydrogels with encapsulated betamethasone revealed greater therapeutic efficacy than the drug applied alone. Therefore, this strategy can provide an improvement in therapeutic efficacy when compared to the traditional use of drugs for the treatment of rheumatoid arthritis. |
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Anti-Inflammatory properties of injectable Betamethasone-Loaded Tyramine-Modified Gellan Gum/Silk Fibroin HydrogelsBetamethasoneEnzymatic crosslinkingInflammationRheumatoid arthritisTy–GG/SF hydrogelsCiências Médicas::Biotecnologia MédicaScience & TechnologyRheumatoid arthritis is a rheumatic disease for which a healing treatment does not presently exist. Silk fibroin has been extensively studied for use in drug delivery systems due to its uniqueness, versatility and strong clinical track record in medicine. However, in general, natural polymeric materials are not mechanically stable enough, and have high rates of biodegradation. Thus, synthetic materials such as gellan gum can be used to produce composite structures with biological signals to promote tissue-specific interactions while providing the desired mechanical properties. In this work, we aimed to produce hydrogels of tyramine-modified gellan gum with silk fibroin (Tyâ GG/SF) via horseradish peroxidase (HRP), with encapsulated betamethasone, to improve the biocompatibility and mechanical properties, and further increase therapeutic efficacy to treat rheumatoid arthritis (RA). The Tyâ GG/SF hydrogels presented a β-sheet secondary structure, with gelation time around 2â 5 min, good resistance to enzymatic degradation, a suitable injectability profile, viscoelastic capacity with a significant solid component and a betamethasone-controlled release profile over time. In vitro studies showed that Tyâ GG/SF hydrogels did not produce a deleterious effect on cellular metabolic activity, morphology or proliferation. Furthermore, Tyâ GG/SF hydrogels with encapsulated betamethasone revealed greater therapeutic efficacy than the drug applied alone. Therefore, this strategy can provide an improvement in therapeutic efficacy when compared to the traditional use of drugs for the treatment of rheumatoid arthritis.Norte2020 project (“NORTE-08-5369-FSE000044”), REMIX project (GA 778078—REMIX—H2020-MSCA-RISE-2017) Gilson Lab, Chonbuk National University, Republic of Korea and the Frontiers of technology for theranostics of cancer, metabolic and neurodegenerative diseases (FROnTHERA), Structural Project NORTE-01-0145-FEDER-000023. The FCT distinction attributed to J.M.O. under the Investigator FCT program (number IF/01285/2015) is also greatly acknowledged. C.G. also wishes to acknowledge FCT for supporting her research (number SFRH/BPD/94277/2013MDPI PublishingUniversidade do MinhoOliveira, Isabel Maria Lopes MatosGonçalves, C.Shin, M. E.Lee, S.Reis, R. L.Khang, G.Oliveira, J. M.2020-102020-10-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/67962engOliveira I. M., Gonçalves C., Shin M. E., Lee S., Reis R. L., Khang G., Oliveira J. M. Anti-Inflammatory Properties of Injectable Betamethasone-Loaded Tyramine-Modified Gellan Gum/Silk Fibroin Hydrogels, Biomolecules, Vol. 10, Issue 1456, doi:10.3390/biom10101456, 20202218-273X10.3390/biom1010145633080875https://www.mdpi.com/2218-273X/10/10/1456info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:35:07Zoai:repositorium.sdum.uminho.pt:1822/67962Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:30:56.513820Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Anti-Inflammatory properties of injectable Betamethasone-Loaded Tyramine-Modified Gellan Gum/Silk Fibroin Hydrogels |
title |
Anti-Inflammatory properties of injectable Betamethasone-Loaded Tyramine-Modified Gellan Gum/Silk Fibroin Hydrogels |
spellingShingle |
Anti-Inflammatory properties of injectable Betamethasone-Loaded Tyramine-Modified Gellan Gum/Silk Fibroin Hydrogels Oliveira, Isabel Maria Lopes Matos Betamethasone Enzymatic crosslinking Inflammation Rheumatoid arthritis Ty–GG/SF hydrogels Ciências Médicas::Biotecnologia Médica Science & Technology |
title_short |
Anti-Inflammatory properties of injectable Betamethasone-Loaded Tyramine-Modified Gellan Gum/Silk Fibroin Hydrogels |
title_full |
Anti-Inflammatory properties of injectable Betamethasone-Loaded Tyramine-Modified Gellan Gum/Silk Fibroin Hydrogels |
title_fullStr |
Anti-Inflammatory properties of injectable Betamethasone-Loaded Tyramine-Modified Gellan Gum/Silk Fibroin Hydrogels |
title_full_unstemmed |
Anti-Inflammatory properties of injectable Betamethasone-Loaded Tyramine-Modified Gellan Gum/Silk Fibroin Hydrogels |
title_sort |
Anti-Inflammatory properties of injectable Betamethasone-Loaded Tyramine-Modified Gellan Gum/Silk Fibroin Hydrogels |
author |
Oliveira, Isabel Maria Lopes Matos |
author_facet |
Oliveira, Isabel Maria Lopes Matos Gonçalves, C. Shin, M. E. Lee, S. Reis, R. L. Khang, G. Oliveira, J. M. |
author_role |
author |
author2 |
Gonçalves, C. Shin, M. E. Lee, S. Reis, R. L. Khang, G. Oliveira, J. M. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Oliveira, Isabel Maria Lopes Matos Gonçalves, C. Shin, M. E. Lee, S. Reis, R. L. Khang, G. Oliveira, J. M. |
dc.subject.por.fl_str_mv |
Betamethasone Enzymatic crosslinking Inflammation Rheumatoid arthritis Ty–GG/SF hydrogels Ciências Médicas::Biotecnologia Médica Science & Technology |
topic |
Betamethasone Enzymatic crosslinking Inflammation Rheumatoid arthritis Ty–GG/SF hydrogels Ciências Médicas::Biotecnologia Médica Science & Technology |
description |
Rheumatoid arthritis is a rheumatic disease for which a healing treatment does not presently exist. Silk fibroin has been extensively studied for use in drug delivery systems due to its uniqueness, versatility and strong clinical track record in medicine. However, in general, natural polymeric materials are not mechanically stable enough, and have high rates of biodegradation. Thus, synthetic materials such as gellan gum can be used to produce composite structures with biological signals to promote tissue-specific interactions while providing the desired mechanical properties. In this work, we aimed to produce hydrogels of tyramine-modified gellan gum with silk fibroin (Tyâ GG/SF) via horseradish peroxidase (HRP), with encapsulated betamethasone, to improve the biocompatibility and mechanical properties, and further increase therapeutic efficacy to treat rheumatoid arthritis (RA). The Tyâ GG/SF hydrogels presented a β-sheet secondary structure, with gelation time around 2â 5 min, good resistance to enzymatic degradation, a suitable injectability profile, viscoelastic capacity with a significant solid component and a betamethasone-controlled release profile over time. In vitro studies showed that Tyâ GG/SF hydrogels did not produce a deleterious effect on cellular metabolic activity, morphology or proliferation. Furthermore, Tyâ GG/SF hydrogels with encapsulated betamethasone revealed greater therapeutic efficacy than the drug applied alone. Therefore, this strategy can provide an improvement in therapeutic efficacy when compared to the traditional use of drugs for the treatment of rheumatoid arthritis. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-10 2020-10-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/67962 |
url |
http://hdl.handle.net/1822/67962 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Oliveira I. M., Gonçalves C., Shin M. E., Lee S., Reis R. L., Khang G., Oliveira J. M. Anti-Inflammatory Properties of Injectable Betamethasone-Loaded Tyramine-Modified Gellan Gum/Silk Fibroin Hydrogels, Biomolecules, Vol. 10, Issue 1456, doi:10.3390/biom10101456, 2020 2218-273X 10.3390/biom10101456 33080875 https://www.mdpi.com/2218-273X/10/10/1456 |
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info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI Publishing |
publisher.none.fl_str_mv |
MDPI Publishing |
dc.source.none.fl_str_mv |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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