Micropropagation of Melissa officinalis L. through proliferation of axillary shoots
Autor(a) principal: | |
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Data de Publicação: | 1996 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/15687 https://doi.org/10.1007/BF00232072 |
Resumo: | Multiple shoots were differentiated in cotyledonary nodes of 10 d old seedlings of Melissa officinalis, cultured on MS medium supplemented with BAP (0-4 mg/l). The production of shoots was further induced in subcultures of the original explant, after the first harvest of shoots (stumps), using similar conditions. The highest average number of shoots in the two inoculations was obtained with 2 mg/l of BAP: 24 axillary shoots per explant, 7 in the first inoculation and 17 in the second one. The maximum elongation of shoots was achieved with BAP at 0.2 mg/l, and higher concentrations of the hormone induced a decrease in their size. A range of BAP concentrations between 0.2 - 0.5 mg/l allowed the production of more shoots with a size suitable for rooting. Roots were induced in 30d old shoots, transferred to MS medium individually supplemented with IBA or NAA (0 - 4 mg/l). Micropropagated plants were successfully transferred to soil. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Micropropagation of Melissa officinalis L. through proliferation of axillary shootsMicropropagaçãoMelissa officinalisMultiple shoots were differentiated in cotyledonary nodes of 10 d old seedlings of Melissa officinalis, cultured on MS medium supplemented with BAP (0-4 mg/l). The production of shoots was further induced in subcultures of the original explant, after the first harvest of shoots (stumps), using similar conditions. The highest average number of shoots in the two inoculations was obtained with 2 mg/l of BAP: 24 axillary shoots per explant, 7 in the first inoculation and 17 in the second one. The maximum elongation of shoots was achieved with BAP at 0.2 mg/l, and higher concentrations of the hormone induced a decrease in their size. A range of BAP concentrations between 0.2 - 0.5 mg/l allowed the production of more shoots with a size suitable for rooting. Roots were induced in 30d old shoots, transferred to MS medium individually supplemented with IBA or NAA (0 - 4 mg/l). Micropropagated plants were successfully transferred to soil.Springer - Verlag1996info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/15687http://hdl.handle.net/10316/15687https://doi.org/10.1007/BF00232072engTavares, A. C.Pimenta, M. C.Gonçalves, M. T.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2021-09-03T08:29:51ZPortal AgregadorONG |
dc.title.none.fl_str_mv |
Micropropagation of Melissa officinalis L. through proliferation of axillary shoots |
title |
Micropropagation of Melissa officinalis L. through proliferation of axillary shoots |
spellingShingle |
Micropropagation of Melissa officinalis L. through proliferation of axillary shoots Tavares, A. C. Micropropagação Melissa officinalis |
title_short |
Micropropagation of Melissa officinalis L. through proliferation of axillary shoots |
title_full |
Micropropagation of Melissa officinalis L. through proliferation of axillary shoots |
title_fullStr |
Micropropagation of Melissa officinalis L. through proliferation of axillary shoots |
title_full_unstemmed |
Micropropagation of Melissa officinalis L. through proliferation of axillary shoots |
title_sort |
Micropropagation of Melissa officinalis L. through proliferation of axillary shoots |
author |
Tavares, A. C. |
author_facet |
Tavares, A. C. Pimenta, M. C. Gonçalves, M. T. |
author_role |
author |
author2 |
Pimenta, M. C. Gonçalves, M. T. |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Tavares, A. C. Pimenta, M. C. Gonçalves, M. T. |
dc.subject.por.fl_str_mv |
Micropropagação Melissa officinalis |
topic |
Micropropagação Melissa officinalis |
description |
Multiple shoots were differentiated in cotyledonary nodes of 10 d old seedlings of Melissa officinalis, cultured on MS medium supplemented with BAP (0-4 mg/l). The production of shoots was further induced in subcultures of the original explant, after the first harvest of shoots (stumps), using similar conditions. The highest average number of shoots in the two inoculations was obtained with 2 mg/l of BAP: 24 axillary shoots per explant, 7 in the first inoculation and 17 in the second one. The maximum elongation of shoots was achieved with BAP at 0.2 mg/l, and higher concentrations of the hormone induced a decrease in their size. A range of BAP concentrations between 0.2 - 0.5 mg/l allowed the production of more shoots with a size suitable for rooting. Roots were induced in 30d old shoots, transferred to MS medium individually supplemented with IBA or NAA (0 - 4 mg/l). Micropropagated plants were successfully transferred to soil. |
publishDate |
1996 |
dc.date.none.fl_str_mv |
1996 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/15687 http://hdl.handle.net/10316/15687 https://doi.org/10.1007/BF00232072 |
url |
http://hdl.handle.net/10316/15687 https://doi.org/10.1007/BF00232072 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Springer - Verlag |
publisher.none.fl_str_mv |
Springer - Verlag |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
|
repository.mail.fl_str_mv |
|
_version_ |
1777302653299064832 |