Whole-Genome Sequence Analysis of Multidrug-Resistant Campylobacter Isolates: a Focus on Aminoglycoside Resistance Determinants

Detalhes bibliográficos
Autor(a) principal: Fabre, Adrien
Data de Publicação: 2018
Outros Autores: Oleastro, Monica, Nunes, Alexandra, Santos, Andrea, Sifré, Elodie, Ducournau, Astrid, Bénéjat, Lucie, Buissonnière, Alice, Floch, Pauline, Mégraud, Francis, Dubois, Véronique, Lehours, Philippe
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.18/6257
Resumo: Free PMC Article: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6113490/
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spelling Whole-Genome Sequence Analysis of Multidrug-Resistant Campylobacter Isolates: a Focus on Aminoglycoside Resistance DeterminantsCampylobacterAntibiotic ResistanceAminoglycoside ResistanceAminoglycoside-modifying EnzymesMultidrug ResistanceWhole-genome SequencingInfecções GastrointestinaisFree PMC Article: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6113490/A (WGS) approach was conducted in order to identify the molecular determinants associated with antimicrobial resistance in 12 multidrug-resistant Campylobacter jejuni and Campylobacter coli isolates, with a focus on aminoglycoside resistance determinants. Two variants of a new aminoglycoside phosphotransferase gene [aph(2″)-Ii1 and aph(2″)-Ii2 ] putatively associated with gentamicin resistance were found. In addition, the following new genes were identified for the first time in Campylobacter: a lincosamide nucleotidyltransferase gene [lnu(G)], likely associated with lincomycin resistance, and two resistance enzyme genes (spw and apmA) similar to those found in Staphylococcus aureus, which may confer spectinomycin and gentamicin resistance, respectively. A C1192T mutation of the 16S rRNA gene that may be involved in spectinomycin resistance was also found in a C. coli isolate. Genes identified in the present study were located either on the bacterial chromosome or on plasmids that could be transferred naturally. Their role in aminoglycoside resistance remains to be supported by genetic studies. Regarding the other antimicrobial agents studied, i.e., ampicillin, ciprofloxacin, erythromycin, and tetracycline, a perfect correlation between antimicrobial phenotypes and genotypes was found. Overall, our data suggest that WGS analysis is a powerful tool for identifying resistance determinants in Campylobacter and can disclose the full genetic elements associated with resistance, including antimicrobial compounds not tested routinely in antimicrobial susceptibility testing.This work was supported by internal funding of the Ricardo Jorge National Institute of Health. WGS was performed at the Unidade de Tecnologia e Inovação (Departamento de Genética Humana, Instituto Nacional de Saúde Ricardo Jorge, Lisbon, Portugal).American Society for MicrobiologyRepositório Científico do Instituto Nacional de SaúdeFabre, AdrienOleastro, MonicaNunes, AlexandraSantos, AndreaSifré, ElodieDucournau, AstridBénéjat, LucieBuissonnière, AliceFloch, PaulineMégraud, FrancisDubois, VéroniqueLehours, Philippe2019-03-20T18:22:07Z2018-08-272018-08-27T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/6257engJ Clin Microbiol. 2018 Aug 27;56(9). pii: e00390-18. doi: 10.1128/JCM.00390-180095-113710.1128/JCM.00390-18info:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:41:17Zoai:repositorio.insa.pt:10400.18/6257Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:40:53.025388Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Whole-Genome Sequence Analysis of Multidrug-Resistant Campylobacter Isolates: a Focus on Aminoglycoside Resistance Determinants
title Whole-Genome Sequence Analysis of Multidrug-Resistant Campylobacter Isolates: a Focus on Aminoglycoside Resistance Determinants
spellingShingle Whole-Genome Sequence Analysis of Multidrug-Resistant Campylobacter Isolates: a Focus on Aminoglycoside Resistance Determinants
Fabre, Adrien
Campylobacter
Antibiotic Resistance
Aminoglycoside Resistance
Aminoglycoside-modifying Enzymes
Multidrug Resistance
Whole-genome Sequencing
Infecções Gastrointestinais
title_short Whole-Genome Sequence Analysis of Multidrug-Resistant Campylobacter Isolates: a Focus on Aminoglycoside Resistance Determinants
title_full Whole-Genome Sequence Analysis of Multidrug-Resistant Campylobacter Isolates: a Focus on Aminoglycoside Resistance Determinants
title_fullStr Whole-Genome Sequence Analysis of Multidrug-Resistant Campylobacter Isolates: a Focus on Aminoglycoside Resistance Determinants
title_full_unstemmed Whole-Genome Sequence Analysis of Multidrug-Resistant Campylobacter Isolates: a Focus on Aminoglycoside Resistance Determinants
title_sort Whole-Genome Sequence Analysis of Multidrug-Resistant Campylobacter Isolates: a Focus on Aminoglycoside Resistance Determinants
author Fabre, Adrien
author_facet Fabre, Adrien
Oleastro, Monica
Nunes, Alexandra
Santos, Andrea
Sifré, Elodie
Ducournau, Astrid
Bénéjat, Lucie
Buissonnière, Alice
Floch, Pauline
Mégraud, Francis
Dubois, Véronique
Lehours, Philippe
author_role author
author2 Oleastro, Monica
Nunes, Alexandra
Santos, Andrea
Sifré, Elodie
Ducournau, Astrid
Bénéjat, Lucie
Buissonnière, Alice
Floch, Pauline
Mégraud, Francis
Dubois, Véronique
Lehours, Philippe
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Nacional de Saúde
dc.contributor.author.fl_str_mv Fabre, Adrien
Oleastro, Monica
Nunes, Alexandra
Santos, Andrea
Sifré, Elodie
Ducournau, Astrid
Bénéjat, Lucie
Buissonnière, Alice
Floch, Pauline
Mégraud, Francis
Dubois, Véronique
Lehours, Philippe
dc.subject.por.fl_str_mv Campylobacter
Antibiotic Resistance
Aminoglycoside Resistance
Aminoglycoside-modifying Enzymes
Multidrug Resistance
Whole-genome Sequencing
Infecções Gastrointestinais
topic Campylobacter
Antibiotic Resistance
Aminoglycoside Resistance
Aminoglycoside-modifying Enzymes
Multidrug Resistance
Whole-genome Sequencing
Infecções Gastrointestinais
description Free PMC Article: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6113490/
publishDate 2018
dc.date.none.fl_str_mv 2018-08-27
2018-08-27T00:00:00Z
2019-03-20T18:22:07Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.18/6257
url http://hdl.handle.net/10400.18/6257
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv J Clin Microbiol. 2018 Aug 27;56(9). pii: e00390-18. doi: 10.1128/JCM.00390-18
0095-1137
10.1128/JCM.00390-18
dc.rights.driver.fl_str_mv info:eu-repo/semantics/embargoedAccess
eu_rights_str_mv embargoedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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