Chitosan-reinforced alginate microspheres obtained through the emulsification/internal gelation technique

Detalhes bibliográficos
Autor(a) principal: Ribeiro, António J.
Data de Publicação: 2005
Outros Autores: Silva, Catarina, Ferreira, Domingos, Veiga, Francisco
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10316/5752
https://doi.org/10.1016/j.ejps.2005.01.016
Resumo: Alginate microspheres prepared by emulsification/internal gelation were chosen as carriers for a model protein, hemoglobin (Hb). Reinforced chitosan-coated microspheres were obtained by an uninterrupted method, in order to simplify the coating process, minimize protein losses during production and to avoid Hb escape under acidic conditions. Microspheres recovery was evaluated as well as its morphology by determination of Hb encapsulation efficiency and microscopic observation, respectively. The formation of chitosan membrane made of it interaction with alginate was assessed by DSC (differential scanning calorimetry) and FT-IR (Fourier-transform infrared spectrometry) studies. Spherical uncoated microspheres with a mean diameter of 20 [mu]m and encapsulation efficiency above 89% were obtained. Coated microspheres provided similar encapsulation efficiency but a higher mean diameter was obtained due to microspheres clumping during the coating step. Protein loss occurred mainly during emulsification rather than recovery. FT-IR and DSC together indicated electrostatic interactions between alginate carboxylate and chitosan ammonium groups as the main forces for complex formation.
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spelling Chitosan-reinforced alginate microspheres obtained through the emulsification/internal gelation techniqueAlginateChitosanInternal gelationMicrospheresOral protein deliveryAlginate microspheres prepared by emulsification/internal gelation were chosen as carriers for a model protein, hemoglobin (Hb). Reinforced chitosan-coated microspheres were obtained by an uninterrupted method, in order to simplify the coating process, minimize protein losses during production and to avoid Hb escape under acidic conditions. Microspheres recovery was evaluated as well as its morphology by determination of Hb encapsulation efficiency and microscopic observation, respectively. The formation of chitosan membrane made of it interaction with alginate was assessed by DSC (differential scanning calorimetry) and FT-IR (Fourier-transform infrared spectrometry) studies. Spherical uncoated microspheres with a mean diameter of 20 [mu]m and encapsulation efficiency above 89% were obtained. Coated microspheres provided similar encapsulation efficiency but a higher mean diameter was obtained due to microspheres clumping during the coating step. Protein loss occurred mainly during emulsification rather than recovery. FT-IR and DSC together indicated electrostatic interactions between alginate carboxylate and chitosan ammonium groups as the main forces for complex formation.http://www.sciencedirect.com/science/article/B6T25-4FM5CXS-1/1/2db2be842f728a0541def21ac050a44e2005info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleaplication/PDFhttp://hdl.handle.net/10316/5752http://hdl.handle.net/10316/5752https://doi.org/10.1016/j.ejps.2005.01.016engEuropean Journal of Pharmaceutical Sciences. 25:1 (2005) 31-40Ribeiro, António J.Silva, CatarinaFerreira, DomingosVeiga, Franciscoinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2020-11-06T16:49:01ZPortal AgregadorONG
dc.title.none.fl_str_mv Chitosan-reinforced alginate microspheres obtained through the emulsification/internal gelation technique
title Chitosan-reinforced alginate microspheres obtained through the emulsification/internal gelation technique
spellingShingle Chitosan-reinforced alginate microspheres obtained through the emulsification/internal gelation technique
Ribeiro, António J.
Alginate
Chitosan
Internal gelation
Microspheres
Oral protein delivery
title_short Chitosan-reinforced alginate microspheres obtained through the emulsification/internal gelation technique
title_full Chitosan-reinforced alginate microspheres obtained through the emulsification/internal gelation technique
title_fullStr Chitosan-reinforced alginate microspheres obtained through the emulsification/internal gelation technique
title_full_unstemmed Chitosan-reinforced alginate microspheres obtained through the emulsification/internal gelation technique
title_sort Chitosan-reinforced alginate microspheres obtained through the emulsification/internal gelation technique
author Ribeiro, António J.
author_facet Ribeiro, António J.
Silva, Catarina
Ferreira, Domingos
Veiga, Francisco
author_role author
author2 Silva, Catarina
Ferreira, Domingos
Veiga, Francisco
author2_role author
author
author
dc.contributor.author.fl_str_mv Ribeiro, António J.
Silva, Catarina
Ferreira, Domingos
Veiga, Francisco
dc.subject.por.fl_str_mv Alginate
Chitosan
Internal gelation
Microspheres
Oral protein delivery
topic Alginate
Chitosan
Internal gelation
Microspheres
Oral protein delivery
description Alginate microspheres prepared by emulsification/internal gelation were chosen as carriers for a model protein, hemoglobin (Hb). Reinforced chitosan-coated microspheres were obtained by an uninterrupted method, in order to simplify the coating process, minimize protein losses during production and to avoid Hb escape under acidic conditions. Microspheres recovery was evaluated as well as its morphology by determination of Hb encapsulation efficiency and microscopic observation, respectively. The formation of chitosan membrane made of it interaction with alginate was assessed by DSC (differential scanning calorimetry) and FT-IR (Fourier-transform infrared spectrometry) studies. Spherical uncoated microspheres with a mean diameter of 20 [mu]m and encapsulation efficiency above 89% were obtained. Coated microspheres provided similar encapsulation efficiency but a higher mean diameter was obtained due to microspheres clumping during the coating step. Protein loss occurred mainly during emulsification rather than recovery. FT-IR and DSC together indicated electrostatic interactions between alginate carboxylate and chitosan ammonium groups as the main forces for complex formation.
publishDate 2005
dc.date.none.fl_str_mv 2005
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10316/5752
http://hdl.handle.net/10316/5752
https://doi.org/10.1016/j.ejps.2005.01.016
url http://hdl.handle.net/10316/5752
https://doi.org/10.1016/j.ejps.2005.01.016
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv European Journal of Pharmaceutical Sciences. 25:1 (2005) 31-40
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv aplication/PDF
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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