Optimal fermentation conditions for maximizing the ethanol production by Kluyveromyces fragilisfrom cheese whey powder

Detalhes bibliográficos
Autor(a) principal: Dragone, Giuliano
Data de Publicação: 2011
Outros Autores: Mussatto, Solange I., Silva, João B. Almeida e, Teixeira, J. A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/16593
Resumo: Cheese whey powder (CWP) is an attractive raw material for ethanol production since it is a dried and concentrated form of CW and contains lactose in addition to nitrogen, phosphate and other essential nutrients. In the present work, deproteinized CWP was utilized as fermentation medium for ethanol production by Kluyveromyces fragilis. The individual and combined effects of initial lactose concentration (50-150 kg m-3), temperature (25-35 ºC) and inoculum concentration (1-3 kg m-3) were investigated through a 23 full factorial central composite design, and the optimal conditions for maximizing the ethanol production were determined. According to the statistical analysis, in the studied range of values, only the initial lactose concentration had a significant effect on ethanol production, resulting in higher product formation as the initial substrate concentration was increased. Assays with initial lactose concentration varying from 150 to 250 kg m-3 were thus performed and revealed that the use of 200 kg m-3 initial lactose concentration, inoculum concentration of 1 kg m-3 and temperature of 35 ºC were the best conditions for maximizing the ethanol production from CWP solution. Under these conditions, 80.95 kg m-3 of ethanol was obtained after 44 h of fermentation.
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spelling Optimal fermentation conditions for maximizing the ethanol production by Kluyveromyces fragilisfrom cheese whey powderBiofuelsCheese wheyEthanolFermentationKluyveromyces fragilisLactoseScience & TechnologyCheese whey powder (CWP) is an attractive raw material for ethanol production since it is a dried and concentrated form of CW and contains lactose in addition to nitrogen, phosphate and other essential nutrients. In the present work, deproteinized CWP was utilized as fermentation medium for ethanol production by Kluyveromyces fragilis. The individual and combined effects of initial lactose concentration (50-150 kg m-3), temperature (25-35 ºC) and inoculum concentration (1-3 kg m-3) were investigated through a 23 full factorial central composite design, and the optimal conditions for maximizing the ethanol production were determined. According to the statistical analysis, in the studied range of values, only the initial lactose concentration had a significant effect on ethanol production, resulting in higher product formation as the initial substrate concentration was increased. Assays with initial lactose concentration varying from 150 to 250 kg m-3 were thus performed and revealed that the use of 200 kg m-3 initial lactose concentration, inoculum concentration of 1 kg m-3 and temperature of 35 ºC were the best conditions for maximizing the ethanol production from CWP solution. Under these conditions, 80.95 kg m-3 of ethanol was obtained after 44 h of fermentation.The authors acknowledge the financial support from "CAPES/Grices (BEX2150/07-7) and Lactogal for supplying cheese whey powder.ElsevierUniversidade do MinhoDragone, GiulianoMussatto, Solange I.Silva, João B. Almeida eTeixeira, J. A.20112011-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/16593eng0961-953410.1016/j.biombioe.2011.01.045http://www.sciencedirect.com/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:06:35Zoai:repositorium.sdum.uminho.pt:1822/16593Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:57:20.387610Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Optimal fermentation conditions for maximizing the ethanol production by Kluyveromyces fragilisfrom cheese whey powder
title Optimal fermentation conditions for maximizing the ethanol production by Kluyveromyces fragilisfrom cheese whey powder
spellingShingle Optimal fermentation conditions for maximizing the ethanol production by Kluyveromyces fragilisfrom cheese whey powder
Dragone, Giuliano
Biofuels
Cheese whey
Ethanol
Fermentation
Kluyveromyces fragilis
Lactose
Science & Technology
title_short Optimal fermentation conditions for maximizing the ethanol production by Kluyveromyces fragilisfrom cheese whey powder
title_full Optimal fermentation conditions for maximizing the ethanol production by Kluyveromyces fragilisfrom cheese whey powder
title_fullStr Optimal fermentation conditions for maximizing the ethanol production by Kluyveromyces fragilisfrom cheese whey powder
title_full_unstemmed Optimal fermentation conditions for maximizing the ethanol production by Kluyveromyces fragilisfrom cheese whey powder
title_sort Optimal fermentation conditions for maximizing the ethanol production by Kluyveromyces fragilisfrom cheese whey powder
author Dragone, Giuliano
author_facet Dragone, Giuliano
Mussatto, Solange I.
Silva, João B. Almeida e
Teixeira, J. A.
author_role author
author2 Mussatto, Solange I.
Silva, João B. Almeida e
Teixeira, J. A.
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Dragone, Giuliano
Mussatto, Solange I.
Silva, João B. Almeida e
Teixeira, J. A.
dc.subject.por.fl_str_mv Biofuels
Cheese whey
Ethanol
Fermentation
Kluyveromyces fragilis
Lactose
Science & Technology
topic Biofuels
Cheese whey
Ethanol
Fermentation
Kluyveromyces fragilis
Lactose
Science & Technology
description Cheese whey powder (CWP) is an attractive raw material for ethanol production since it is a dried and concentrated form of CW and contains lactose in addition to nitrogen, phosphate and other essential nutrients. In the present work, deproteinized CWP was utilized as fermentation medium for ethanol production by Kluyveromyces fragilis. The individual and combined effects of initial lactose concentration (50-150 kg m-3), temperature (25-35 ºC) and inoculum concentration (1-3 kg m-3) were investigated through a 23 full factorial central composite design, and the optimal conditions for maximizing the ethanol production were determined. According to the statistical analysis, in the studied range of values, only the initial lactose concentration had a significant effect on ethanol production, resulting in higher product formation as the initial substrate concentration was increased. Assays with initial lactose concentration varying from 150 to 250 kg m-3 were thus performed and revealed that the use of 200 kg m-3 initial lactose concentration, inoculum concentration of 1 kg m-3 and temperature of 35 ºC were the best conditions for maximizing the ethanol production from CWP solution. Under these conditions, 80.95 kg m-3 of ethanol was obtained after 44 h of fermentation.
publishDate 2011
dc.date.none.fl_str_mv 2011
2011-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/16593
url http://hdl.handle.net/1822/16593
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0961-9534
10.1016/j.biombioe.2011.01.045
http://www.sciencedirect.com/
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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