Modification of microfiltration membranes by hydrogel impregnation for pDNA purification
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/39023 |
Resumo: | The huge efforts for the achievement of highly purified biomolecules are growing every day. A great number of efficient techniques, such as chromatography, are already available in laboratory for separation processes. However, membrane-based technologies are the best match to assure simplicity, efficiency and easy scale-up procedures. Herein we report the modification of a commercial microfiltration membrane for plasmid DNA purification by agarose gel impregnation. The membrane was characterized by SEM, ATR-FTIR, EDS, contact angle, and porosity measurements. Additionally, the membrane pore radius was estimated from observed rejections of different proteins and with that information the rejection of a 6050 bp plasmid DNA (pDNA) molecule was estimated for different values of flux using a theoretical model of large flexible molecules in membranes with parallel cylindrical pores, which is applicable to pDNA ultrafiltration in conventional membranes, as recently shown in the literature. The experimental results show that the modified membrane has higher pDNA rejections than the predicted by the model, suggesting that the different type of porous structure that a hydrogel has, may have a positive effect on pDNA rejections as compared to other biomolecules with more rigid structures, making this type of modified membranes potential better candidates to be used for the selective recovery of pDNA in this type of bioprocesses. |
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Modification of microfiltration membranes by hydrogel impregnation for pDNA purificationMembranesNucleic acidsPolysaccharidesEngenharia e Tecnologia::Engenharia dos MateriaisScience & TechnologyThe huge efforts for the achievement of highly purified biomolecules are growing every day. A great number of efficient techniques, such as chromatography, are already available in laboratory for separation processes. However, membrane-based technologies are the best match to assure simplicity, efficiency and easy scale-up procedures. Herein we report the modification of a commercial microfiltration membrane for plasmid DNA purification by agarose gel impregnation. The membrane was characterized by SEM, ATR-FTIR, EDS, contact angle, and porosity measurements. Additionally, the membrane pore radius was estimated from observed rejections of different proteins and with that information the rejection of a 6050 bp plasmid DNA (pDNA) molecule was estimated for different values of flux using a theoretical model of large flexible molecules in membranes with parallel cylindrical pores, which is applicable to pDNA ultrafiltration in conventional membranes, as recently shown in the literature. The experimental results show that the modified membrane has higher pDNA rejections than the predicted by the model, suggesting that the different type of porous structure that a hydrogel has, may have a positive effect on pDNA rejections as compared to other biomolecules with more rigid structures, making this type of modified membranes potential better candidates to be used for the selective recovery of pDNA in this type of bioprocesses.Fundação para a Ciência e a Tecnologia (FCT), Portugal, for the grant awarded to António Morão (SFRH/BPD/88838/2012). Pest-C/SAU/UI0709/2011 and Pest-OE/SAU/UI0709/2014WileyUniversidade do MinhoCastilho, Pedro H.Correia, Tiago R.Amorim, M. T. Pessoa deEscobar, Isabel C.Queiroz, João A.Correia, Ilídio J.Morão, António M.20152015-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/39023engCastilho, P. H., Correia, T. R., Pessoa De Amorim, M. T., Escobar, I. C., Queiroz, J. A., Correia, I. J., & Morão, A. M. (2015). Modification of microfiltration membranes by hydrogel impregnation for pDNA purification. Journal of Applied Polymer Science, 132(21). doi: 10.1002/app.416100021-899510.1002/app.41610info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:12:06ZPortal AgregadorONG |
dc.title.none.fl_str_mv |
Modification of microfiltration membranes by hydrogel impregnation for pDNA purification |
title |
Modification of microfiltration membranes by hydrogel impregnation for pDNA purification |
spellingShingle |
Modification of microfiltration membranes by hydrogel impregnation for pDNA purification Castilho, Pedro H. Membranes Nucleic acids Polysaccharides Engenharia e Tecnologia::Engenharia dos Materiais Science & Technology |
title_short |
Modification of microfiltration membranes by hydrogel impregnation for pDNA purification |
title_full |
Modification of microfiltration membranes by hydrogel impregnation for pDNA purification |
title_fullStr |
Modification of microfiltration membranes by hydrogel impregnation for pDNA purification |
title_full_unstemmed |
Modification of microfiltration membranes by hydrogel impregnation for pDNA purification |
title_sort |
Modification of microfiltration membranes by hydrogel impregnation for pDNA purification |
author |
Castilho, Pedro H. |
author_facet |
Castilho, Pedro H. Correia, Tiago R. Amorim, M. T. Pessoa de Escobar, Isabel C. Queiroz, João A. Correia, Ilídio J. Morão, António M. |
author_role |
author |
author2 |
Correia, Tiago R. Amorim, M. T. Pessoa de Escobar, Isabel C. Queiroz, João A. Correia, Ilídio J. Morão, António M. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Castilho, Pedro H. Correia, Tiago R. Amorim, M. T. Pessoa de Escobar, Isabel C. Queiroz, João A. Correia, Ilídio J. Morão, António M. |
dc.subject.por.fl_str_mv |
Membranes Nucleic acids Polysaccharides Engenharia e Tecnologia::Engenharia dos Materiais Science & Technology |
topic |
Membranes Nucleic acids Polysaccharides Engenharia e Tecnologia::Engenharia dos Materiais Science & Technology |
description |
The huge efforts for the achievement of highly purified biomolecules are growing every day. A great number of efficient techniques, such as chromatography, are already available in laboratory for separation processes. However, membrane-based technologies are the best match to assure simplicity, efficiency and easy scale-up procedures. Herein we report the modification of a commercial microfiltration membrane for plasmid DNA purification by agarose gel impregnation. The membrane was characterized by SEM, ATR-FTIR, EDS, contact angle, and porosity measurements. Additionally, the membrane pore radius was estimated from observed rejections of different proteins and with that information the rejection of a 6050 bp plasmid DNA (pDNA) molecule was estimated for different values of flux using a theoretical model of large flexible molecules in membranes with parallel cylindrical pores, which is applicable to pDNA ultrafiltration in conventional membranes, as recently shown in the literature. The experimental results show that the modified membrane has higher pDNA rejections than the predicted by the model, suggesting that the different type of porous structure that a hydrogel has, may have a positive effect on pDNA rejections as compared to other biomolecules with more rigid structures, making this type of modified membranes potential better candidates to be used for the selective recovery of pDNA in this type of bioprocesses. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015 2015-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/39023 |
url |
http://hdl.handle.net/1822/39023 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Castilho, P. H., Correia, T. R., Pessoa De Amorim, M. T., Escobar, I. C., Queiroz, J. A., Correia, I. J., & Morão, A. M. (2015). Modification of microfiltration membranes by hydrogel impregnation for pDNA purification. Journal of Applied Polymer Science, 132(21). doi: 10.1002/app.41610 0021-8995 10.1002/app.41610 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Wiley |
publisher.none.fl_str_mv |
Wiley |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
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repository.mail.fl_str_mv |
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1777303703916642304 |