Detection and localization of osteocalcin (BGP or Bone Gla Protein) in teleosts by in situ hybridization and immunohistochemistry

Detalhes bibliográficos
Autor(a) principal: Gavaia, Paulo J.
Data de Publicação: 2001
Outros Autores: Viegas, C. A., Pinto, Jorge, Sarasquete, C., Cancela, Leonor
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.1/4468
Resumo: Although the presence of osteocalcin (BGP) has been known for a number years, little knowledge on the regulation of expression and tissue localization of this protein in lower vertebrate organisms. In this site we have investigated the site of BGP mRNA expression by in situ hybridization using radiolabeled riboprobes and the tissue localization of the mature protein by immunohistochemistry in the Senegal sole (Solea senegalensis), zebrafish (Danio rerio), and gilthead sea bream (Sparus aurata) using fish BGP polyclonal antibodies.
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spelling Detection and localization of osteocalcin (BGP or Bone Gla Protein) in teleosts by in situ hybridization and immunohistochemistryAlthough the presence of osteocalcin (BGP) has been known for a number years, little knowledge on the regulation of expression and tissue localization of this protein in lower vertebrate organisms. In this site we have investigated the site of BGP mRNA expression by in situ hybridization using radiolabeled riboprobes and the tissue localization of the mature protein by immunohistochemistry in the Senegal sole (Solea senegalensis), zebrafish (Danio rerio), and gilthead sea bream (Sparus aurata) using fish BGP polyclonal antibodies.ElsevierSapientiaGavaia, Paulo J.Viegas, C. A.Pinto, JorgeSarasquete, C.Cancela, Leonor2014-06-24T11:27:12Z20012001-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/4468engGavaia, P.J., Viegas, C.A., Pinto, J.P. Sarasquete, C., Cancela, M.L. 2001. Detection and localization of osteocalcin (BGP or Bone Gla Protein) in teleosts by in situ hybridization and immunohistochemistry. Bone, 28 (5) S103, P9.8756-3282AUT: LCA00739info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:15:30Zoai:sapientia.ualg.pt:10400.1/4468Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:57:41.657208Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Detection and localization of osteocalcin (BGP or Bone Gla Protein) in teleosts by in situ hybridization and immunohistochemistry
title Detection and localization of osteocalcin (BGP or Bone Gla Protein) in teleosts by in situ hybridization and immunohistochemistry
spellingShingle Detection and localization of osteocalcin (BGP or Bone Gla Protein) in teleosts by in situ hybridization and immunohistochemistry
Gavaia, Paulo J.
title_short Detection and localization of osteocalcin (BGP or Bone Gla Protein) in teleosts by in situ hybridization and immunohistochemistry
title_full Detection and localization of osteocalcin (BGP or Bone Gla Protein) in teleosts by in situ hybridization and immunohistochemistry
title_fullStr Detection and localization of osteocalcin (BGP or Bone Gla Protein) in teleosts by in situ hybridization and immunohistochemistry
title_full_unstemmed Detection and localization of osteocalcin (BGP or Bone Gla Protein) in teleosts by in situ hybridization and immunohistochemistry
title_sort Detection and localization of osteocalcin (BGP or Bone Gla Protein) in teleosts by in situ hybridization and immunohistochemistry
author Gavaia, Paulo J.
author_facet Gavaia, Paulo J.
Viegas, C. A.
Pinto, Jorge
Sarasquete, C.
Cancela, Leonor
author_role author
author2 Viegas, C. A.
Pinto, Jorge
Sarasquete, C.
Cancela, Leonor
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Sapientia
dc.contributor.author.fl_str_mv Gavaia, Paulo J.
Viegas, C. A.
Pinto, Jorge
Sarasquete, C.
Cancela, Leonor
description Although the presence of osteocalcin (BGP) has been known for a number years, little knowledge on the regulation of expression and tissue localization of this protein in lower vertebrate organisms. In this site we have investigated the site of BGP mRNA expression by in situ hybridization using radiolabeled riboprobes and the tissue localization of the mature protein by immunohistochemistry in the Senegal sole (Solea senegalensis), zebrafish (Danio rerio), and gilthead sea bream (Sparus aurata) using fish BGP polyclonal antibodies.
publishDate 2001
dc.date.none.fl_str_mv 2001
2001-01-01T00:00:00Z
2014-06-24T11:27:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.1/4468
url http://hdl.handle.net/10400.1/4468
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Gavaia, P.J., Viegas, C.A., Pinto, J.P. Sarasquete, C., Cancela, M.L. 2001. Detection and localization of osteocalcin (BGP or Bone Gla Protein) in teleosts by in situ hybridization and immunohistochemistry. Bone, 28 (5) S103, P9.
8756-3282
AUT: LCA00739
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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