Molecular cloning of the gene encoding flavoredoxin, a flavoprotein from Desulfovibrio gigas

Detalhes bibliográficos
Autor(a) principal: Agostinho, Marta
Data de Publicação: 2000
Outros Autores: Oliveira, Solange, Broco, Manuela, Liu, M.Y., LeGall, Jean, Rodrigues-Pousada, Claudina
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10174/2112
Resumo: Sulfate-reducing bacteria are rich in unique redox proteins and electron carriers that participate in a variety of essential pathways. Several studies have been carried out to characterize these proteins, but the structure and function of many are poorly understood. Many Desulfovibrio species can grow using hydrogen as the sole energy source, indicating that the oxidation of hydrogen with sulfite as the terminal electron acceptor is an energy-conserving mechanism. Flavoredoxin is an FMN-binding protein isolated from the sulfate-reducing bacteria Desulfovibrio gigas that participates in the reduction of bisulfite from hydrogen. Here we report the cloning and sequencing of the flavoredoxin gene. The derived amino acid sequence exhibits similarity to several flavoproteins which are members of a new family of flavin reductases suggested to bind FMN in a novel mode. (C) 2000 Academic Press
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spelling Molecular cloning of the gene encoding flavoredoxin, a flavoprotein from Desulfovibrio gigasDesulfovibrio gigasflavoredoxinSulfate-reducing bacteria are rich in unique redox proteins and electron carriers that participate in a variety of essential pathways. Several studies have been carried out to characterize these proteins, but the structure and function of many are poorly understood. Many Desulfovibrio species can grow using hydrogen as the sole energy source, indicating that the oxidation of hydrogen with sulfite as the terminal electron acceptor is an energy-conserving mechanism. Flavoredoxin is an FMN-binding protein isolated from the sulfate-reducing bacteria Desulfovibrio gigas that participates in the reduction of bisulfite from hydrogen. Here we report the cloning and sequencing of the flavoredoxin gene. The derived amino acid sequence exhibits similarity to several flavoproteins which are members of a new family of flavin reductases suggested to bind FMN in a novel mode. (C) 2000 Academic Press2010-10-07T08:58:44Z2010-10-072000-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article86047 bytesapplication/pdfhttp://hdl.handle.net/10174/2112http://hdl.handle.net/10174/2112engpag 653-656272BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONSlivrendndndndndnd552Agostinho, MartaOliveira, SolangeBroco, ManuelaLiu, M.Y.LeGall, JeanRodrigues-Pousada, Claudinainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T18:38:17Zoai:dspace.uevora.pt:10174/2112Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:57:52.890575Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Molecular cloning of the gene encoding flavoredoxin, a flavoprotein from Desulfovibrio gigas
title Molecular cloning of the gene encoding flavoredoxin, a flavoprotein from Desulfovibrio gigas
spellingShingle Molecular cloning of the gene encoding flavoredoxin, a flavoprotein from Desulfovibrio gigas
Agostinho, Marta
Desulfovibrio gigas
flavoredoxin
title_short Molecular cloning of the gene encoding flavoredoxin, a flavoprotein from Desulfovibrio gigas
title_full Molecular cloning of the gene encoding flavoredoxin, a flavoprotein from Desulfovibrio gigas
title_fullStr Molecular cloning of the gene encoding flavoredoxin, a flavoprotein from Desulfovibrio gigas
title_full_unstemmed Molecular cloning of the gene encoding flavoredoxin, a flavoprotein from Desulfovibrio gigas
title_sort Molecular cloning of the gene encoding flavoredoxin, a flavoprotein from Desulfovibrio gigas
author Agostinho, Marta
author_facet Agostinho, Marta
Oliveira, Solange
Broco, Manuela
Liu, M.Y.
LeGall, Jean
Rodrigues-Pousada, Claudina
author_role author
author2 Oliveira, Solange
Broco, Manuela
Liu, M.Y.
LeGall, Jean
Rodrigues-Pousada, Claudina
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Agostinho, Marta
Oliveira, Solange
Broco, Manuela
Liu, M.Y.
LeGall, Jean
Rodrigues-Pousada, Claudina
dc.subject.por.fl_str_mv Desulfovibrio gigas
flavoredoxin
topic Desulfovibrio gigas
flavoredoxin
description Sulfate-reducing bacteria are rich in unique redox proteins and electron carriers that participate in a variety of essential pathways. Several studies have been carried out to characterize these proteins, but the structure and function of many are poorly understood. Many Desulfovibrio species can grow using hydrogen as the sole energy source, indicating that the oxidation of hydrogen with sulfite as the terminal electron acceptor is an energy-conserving mechanism. Flavoredoxin is an FMN-binding protein isolated from the sulfate-reducing bacteria Desulfovibrio gigas that participates in the reduction of bisulfite from hydrogen. Here we report the cloning and sequencing of the flavoredoxin gene. The derived amino acid sequence exhibits similarity to several flavoproteins which are members of a new family of flavin reductases suggested to bind FMN in a novel mode. (C) 2000 Academic Press
publishDate 2000
dc.date.none.fl_str_mv 2000-01-01T00:00:00Z
2010-10-07T08:58:44Z
2010-10-07
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10174/2112
http://hdl.handle.net/10174/2112
url http://hdl.handle.net/10174/2112
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv pag 653-656
272
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
livre
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nd
nd
nd
nd
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552
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