Long-term acclimation of anaerobic sludges for high-rate methanogenesis from LCFA
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/31886 |
Resumo: | Inhibition of methanogens by long chain fatty acids (LCFA) and the low numbers of LCFA-degrading bacteria are limitations to exploit biogas production from fat-rich wastewaters. Generally reactors fail due to excessive LCFA accumulation onto the sludge. Here, long-term acclimation and bioaugmentation with a LCFA-degrading coculture were hypothesized as strategies to enhance methanogenic conversion of these compounds. Anaerobic sludges previously exposed to LCFA for more than 100 days converted a specific biomass-associated substrate of (3.2 ± 0.1) kg·kg−1 with very short lag phases (<1 day), whereas non-acclimated sludges showed lag phases of 11–15 days for metabolizing (1.6–1.8) kg·kg−1. Addition of a coculture of Syntrophomonas zehnderi and Methanobacterium formicicum to sludges previously loaded with LCFA and containing different amounts of biomass-associated substrate (from (0.5–3.2) kg·kg−1) did not improve methane production neither lag phases were shortened, indicating that the endogenous microbiota are not a limiting factor. Clearly, we show that long-term sludge acclimation to LCFA is essential for high rate methanogenesis from LCFA. |
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Long-term acclimation of anaerobic sludges for high-rate methanogenesis from LCFAHigh-rate methanogenesisLong term acclimationSyntrophomonas zehnderiMethanobacterium formicicumLCFAScience & TechnologyInhibition of methanogens by long chain fatty acids (LCFA) and the low numbers of LCFA-degrading bacteria are limitations to exploit biogas production from fat-rich wastewaters. Generally reactors fail due to excessive LCFA accumulation onto the sludge. Here, long-term acclimation and bioaugmentation with a LCFA-degrading coculture were hypothesized as strategies to enhance methanogenic conversion of these compounds. Anaerobic sludges previously exposed to LCFA for more than 100 days converted a specific biomass-associated substrate of (3.2 ± 0.1) kg·kg−1 with very short lag phases (<1 day), whereas non-acclimated sludges showed lag phases of 11–15 days for metabolizing (1.6–1.8) kg·kg−1. Addition of a coculture of Syntrophomonas zehnderi and Methanobacterium formicicum to sludges previously loaded with LCFA and containing different amounts of biomass-associated substrate (from (0.5–3.2) kg·kg−1) did not improve methane production neither lag phases were shortened, indicating that the endogenous microbiota are not a limiting factor. Clearly, we show that long-term sludge acclimation to LCFA is essential for high rate methanogenesis from LCFA.The authors acknowledge the financial support by the European Regional Development Fund - ERDF, through the Operational Program Thematic Factors of Competitiveness - COMPETE, and by Portuguese funds, through the Portuguese Foundation for Science and Technology (FCT), in the frame of the project FCOMP-01-0124-FEDER-014784. FCT Strategic Project PEst-OE/EQB/LA0023/2013 is also acknowledged. A.J. Cavaleiro thanks FCT for the post-doctoral fellowship ref. SFRH/BPD/75247/2010. A.J.M. Stams has received funding from the European Research Council under the European Union's Seventh Framework Programme (FP/2007-2013)/ERC Grant Agreement n. [323009].Pergamon Press Ltd.Universidade do MinhoSilva, SérgioCavaleiro, A. J.Pereira, M. A.Stams, Alfons Johannes MariaAlves, M. M.Sousa, D. Z.20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/31886engSilva, Sérgio; Cavaleiro, A. J.; Pereira, M. A.; Stams, A. J. M.; Alves, M. M.; Sousa, D. Z., Long-term acclimation of anaerobic sludges for high-rate methanogenesis from LCFA. Biomass and Bioenergy, 67(31), 297-303, 20140961-953410.1016/j.biombioe.2014.05.012info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T11:59:04Zoai:repositorium.sdum.uminho.pt:1822/31886Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:48:49.255214Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Long-term acclimation of anaerobic sludges for high-rate methanogenesis from LCFA |
title |
Long-term acclimation of anaerobic sludges for high-rate methanogenesis from LCFA |
spellingShingle |
Long-term acclimation of anaerobic sludges for high-rate methanogenesis from LCFA Silva, Sérgio High-rate methanogenesis Long term acclimation Syntrophomonas zehnderi Methanobacterium formicicum LCFA Science & Technology |
title_short |
Long-term acclimation of anaerobic sludges for high-rate methanogenesis from LCFA |
title_full |
Long-term acclimation of anaerobic sludges for high-rate methanogenesis from LCFA |
title_fullStr |
Long-term acclimation of anaerobic sludges for high-rate methanogenesis from LCFA |
title_full_unstemmed |
Long-term acclimation of anaerobic sludges for high-rate methanogenesis from LCFA |
title_sort |
Long-term acclimation of anaerobic sludges for high-rate methanogenesis from LCFA |
author |
Silva, Sérgio |
author_facet |
Silva, Sérgio Cavaleiro, A. J. Pereira, M. A. Stams, Alfons Johannes Maria Alves, M. M. Sousa, D. Z. |
author_role |
author |
author2 |
Cavaleiro, A. J. Pereira, M. A. Stams, Alfons Johannes Maria Alves, M. M. Sousa, D. Z. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Silva, Sérgio Cavaleiro, A. J. Pereira, M. A. Stams, Alfons Johannes Maria Alves, M. M. Sousa, D. Z. |
dc.subject.por.fl_str_mv |
High-rate methanogenesis Long term acclimation Syntrophomonas zehnderi Methanobacterium formicicum LCFA Science & Technology |
topic |
High-rate methanogenesis Long term acclimation Syntrophomonas zehnderi Methanobacterium formicicum LCFA Science & Technology |
description |
Inhibition of methanogens by long chain fatty acids (LCFA) and the low numbers of LCFA-degrading bacteria are limitations to exploit biogas production from fat-rich wastewaters. Generally reactors fail due to excessive LCFA accumulation onto the sludge. Here, long-term acclimation and bioaugmentation with a LCFA-degrading coculture were hypothesized as strategies to enhance methanogenic conversion of these compounds. Anaerobic sludges previously exposed to LCFA for more than 100 days converted a specific biomass-associated substrate of (3.2 ± 0.1) kg·kg−1 with very short lag phases (<1 day), whereas non-acclimated sludges showed lag phases of 11–15 days for metabolizing (1.6–1.8) kg·kg−1. Addition of a coculture of Syntrophomonas zehnderi and Methanobacterium formicicum to sludges previously loaded with LCFA and containing different amounts of biomass-associated substrate (from (0.5–3.2) kg·kg−1) did not improve methane production neither lag phases were shortened, indicating that the endogenous microbiota are not a limiting factor. Clearly, we show that long-term sludge acclimation to LCFA is essential for high rate methanogenesis from LCFA. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014 2014-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/31886 |
url |
http://hdl.handle.net/1822/31886 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Silva, Sérgio; Cavaleiro, A. J.; Pereira, M. A.; Stams, A. J. M.; Alves, M. M.; Sousa, D. Z., Long-term acclimation of anaerobic sludges for high-rate methanogenesis from LCFA. Biomass and Bioenergy, 67(31), 297-303, 2014 0961-9534 10.1016/j.biombioe.2014.05.012 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Pergamon Press Ltd. |
publisher.none.fl_str_mv |
Pergamon Press Ltd. |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799132251096612864 |