Metalaxyl degradation by Mucorales strains Gongronella sp. and Rhizopus oryzae

Detalhes bibliográficos
Autor(a) principal: Martins, M. Rosário
Data de Publicação: 2017
Outros Autores: Santos, Cledir, Pereira, Pablo, Cruz-Morais, Júlio, Lima, Nelson
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/48441
Resumo: In this study, the degradation of metalaxyl was investigated in the presence of two Mucorales strains, previously isolated from soil subjected to repeated treatments with this fungicide and selected after enrichment technique. Fungal strains were characterised by a polyphasic approach using phylogenetic analysis of the Internal Transcribed Spacer (ITS) gene region, phenotypic characterisation by Matrix-Assisted Laser Desorption Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) spectral analysis, and growth kinetics experiments. The strains were identified as Gongronella sp. and Rhizopus oryzae. The fungal growth kinetics in liquid cultures containing metalaxyl fits with Haldane model. Under laboratory conditions, the ability of Gongronella sp. and R. oryzae cultures to degrade metalaxyl was evaluated in liquid cultures and soil experiments. Both species were able to: (a) use metalaxyl as the main carbon and energy source; and (b) degrade metalaxyl in polluted soils, with rates around 1.0 mg kg1 d1. This suggests these strains could degrade metalaxyl in soils contaminated with this fungicide.
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spelling Metalaxyl degradation by Mucorales strains Gongronella sp. and Rhizopus oryzaeAcylanilide fungicideBioremediationContaminated soilsFungiITS phylogenyMALDI-TOF MSScience & TechnologyIn this study, the degradation of metalaxyl was investigated in the presence of two Mucorales strains, previously isolated from soil subjected to repeated treatments with this fungicide and selected after enrichment technique. Fungal strains were characterised by a polyphasic approach using phylogenetic analysis of the Internal Transcribed Spacer (ITS) gene region, phenotypic characterisation by Matrix-Assisted Laser Desorption Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) spectral analysis, and growth kinetics experiments. The strains were identified as Gongronella sp. and Rhizopus oryzae. The fungal growth kinetics in liquid cultures containing metalaxyl fits with Haldane model. Under laboratory conditions, the ability of Gongronella sp. and R. oryzae cultures to degrade metalaxyl was evaluated in liquid cultures and soil experiments. Both species were able to: (a) use metalaxyl as the main carbon and energy source; and (b) degrade metalaxyl in polluted soils, with rates around 1.0 mg kg1 d1. This suggests these strains could degrade metalaxyl in soils contaminated with this fungicide.This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of “HIT3CH-HERCULES Interface for Technology Transfer and Teaming in Cultural Heritage”, (ALT20-03-0246-FEDER-000004) under the scope of ALENTEJO 2020- Regional Operational Program of Alentejo and BioTecNorte operation (NORTE-01-0145-FEDER-000004) under the scope of Norte 2020 Regional Operational Program of Norte, funded by European Regional Development Fund. C. S. thanks to the University of La Frontera (Temuco, Chile) for the partial funding from Project DIUFRO DI16-0135.info:eu-repo/semantics/publishedVersionMultidisciplinary Digital Publishing Institute (MDPI)Universidade do MinhoMartins, M. RosárioSantos, CledirPereira, PabloCruz-Morais, JúlioLima, Nelson20172017-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/48441engMartins, M. Rosário; Santos, Cledir; Pereira, Pablo; Cruz-Morais, Júlio; Lima, Nelson, Metalaxyl degradation by Mucorales strains Gongronella sp. and Rhizopus oryzae. Biomolecules, 22(2225), 1-12, 20171420-30492218-273X10.3390/molecules2212222529240696info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:18:16Zoai:repositorium.sdum.uminho.pt:1822/48441Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:11:04.617884Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Metalaxyl degradation by Mucorales strains Gongronella sp. and Rhizopus oryzae
title Metalaxyl degradation by Mucorales strains Gongronella sp. and Rhizopus oryzae
spellingShingle Metalaxyl degradation by Mucorales strains Gongronella sp. and Rhizopus oryzae
Martins, M. Rosário
Acylanilide fungicide
Bioremediation
Contaminated soils
Fungi
ITS phylogeny
MALDI-TOF MS
Science & Technology
title_short Metalaxyl degradation by Mucorales strains Gongronella sp. and Rhizopus oryzae
title_full Metalaxyl degradation by Mucorales strains Gongronella sp. and Rhizopus oryzae
title_fullStr Metalaxyl degradation by Mucorales strains Gongronella sp. and Rhizopus oryzae
title_full_unstemmed Metalaxyl degradation by Mucorales strains Gongronella sp. and Rhizopus oryzae
title_sort Metalaxyl degradation by Mucorales strains Gongronella sp. and Rhizopus oryzae
author Martins, M. Rosário
author_facet Martins, M. Rosário
Santos, Cledir
Pereira, Pablo
Cruz-Morais, Júlio
Lima, Nelson
author_role author
author2 Santos, Cledir
Pereira, Pablo
Cruz-Morais, Júlio
Lima, Nelson
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Martins, M. Rosário
Santos, Cledir
Pereira, Pablo
Cruz-Morais, Júlio
Lima, Nelson
dc.subject.por.fl_str_mv Acylanilide fungicide
Bioremediation
Contaminated soils
Fungi
ITS phylogeny
MALDI-TOF MS
Science & Technology
topic Acylanilide fungicide
Bioremediation
Contaminated soils
Fungi
ITS phylogeny
MALDI-TOF MS
Science & Technology
description In this study, the degradation of metalaxyl was investigated in the presence of two Mucorales strains, previously isolated from soil subjected to repeated treatments with this fungicide and selected after enrichment technique. Fungal strains were characterised by a polyphasic approach using phylogenetic analysis of the Internal Transcribed Spacer (ITS) gene region, phenotypic characterisation by Matrix-Assisted Laser Desorption Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) spectral analysis, and growth kinetics experiments. The strains were identified as Gongronella sp. and Rhizopus oryzae. The fungal growth kinetics in liquid cultures containing metalaxyl fits with Haldane model. Under laboratory conditions, the ability of Gongronella sp. and R. oryzae cultures to degrade metalaxyl was evaluated in liquid cultures and soil experiments. Both species were able to: (a) use metalaxyl as the main carbon and energy source; and (b) degrade metalaxyl in polluted soils, with rates around 1.0 mg kg1 d1. This suggests these strains could degrade metalaxyl in soils contaminated with this fungicide.
publishDate 2017
dc.date.none.fl_str_mv 2017
2017-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/48441
url http://hdl.handle.net/1822/48441
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Martins, M. Rosário; Santos, Cledir; Pereira, Pablo; Cruz-Morais, Júlio; Lima, Nelson, Metalaxyl degradation by Mucorales strains Gongronella sp. and Rhizopus oryzae. Biomolecules, 22(2225), 1-12, 2017
1420-3049
2218-273X
10.3390/molecules22122225
29240696
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute (MDPI)
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute (MDPI)
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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