Suitability of the In Vitro Cytokinesis-Block Micronucleus Test for Genotoxicity Assessment of TiO2 Nanoparticles on SH-SY5Y Cells

Detalhes bibliográficos
Autor(a) principal: Fernández-Bertólez, Natalia
Data de Publicação: 2021
Outros Autores: Brandão, Fátima, Costa, Carla, Pásaro, Eduardo, Teixeira, João Paulo, Laffon, Blanca, Valdiglesias, Vanessa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.18/8077
Resumo: (This article belongs to the Special Issue Toxicity of Nanomaterials and Legacy Contaminants: Risks to the Environment and Human Health)
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spelling Suitability of the In Vitro Cytokinesis-Block Micronucleus Test for Genotoxicity Assessment of TiO2 Nanoparticles on SH-SY5Y CellsSH-SY5Y CellsTiO2 NanoparticlesCytochalasin-BCytokinesis-Block Micronucleus TestFlow Cytometry Micronucleus TestNanomaterialsUptakeGenotoxicidade Ambiental(This article belongs to the Special Issue Toxicity of Nanomaterials and Legacy Contaminants: Risks to the Environment and Human Health)Standard toxicity tests might not be fully adequate for evaluating nanomaterials since their unique features are also responsible for unexpected interactions. The in vitro cytokinesis-block micronucleus (CBMN) test is recommended for genotoxicity testing, but cytochalasin-B (Cyt-B) may interfere with nanoparticles (NP), leading to inaccurate results. Our objective was to determine whether Cyt-B could interfere with MN induction by TiO2 NP in human SH-SY5Y cells, as assessed by CBMN test. Cells were treated for 6 or 24 h, according to three treatment options: co-treatment with Cyt-B, post-treatment, and delayed co-treatment. Influence of Cyt-B on TiO2 NP cellular uptake and MN induction as evaluated by flow cytometry (FCMN) were also assessed. TiO2 NP were significantly internalized by cells, both in the absence and presence of Cyt-B, indicating that this chemical does not interfere with NP uptake. Dose-dependent increases in MN rates were observed in CBMN test after co-treatment. However, FCMN assay only showed a positive response when Cyt-B was added simultaneously with TiO2 NP, suggesting that Cyt-B might alter CBMN assay results. No differences were observed in the comparisons between the treatment options assessed, suggesting they are not adequate alternatives to avoid Cyt-B interference in the specific conditions tested.This research was funded by Ministerio de Ciencia e Innovación (PID2020-114908GA-I00), Xunta de Galicia (ED431B 2019/02); NanoBioBarriers (PTDC/MED-TOX/31162/2017) funded by Operational Program for Competitiveness and Internationalisation through European Regional Development Funds (FEDER/FNR) and through national funds by the Portuguese Foundation for Science and Technology (FCT); NanoLegaTox (PTDC/SAU-PUB/29651/2017) project co-financed by COMPETE 2020, Portugal 2020 and European Union, through FEDER; Ministerio de Educación, Cultura y Deporte (BEAGAL18/00142, to V.V.); FCT (SFRH/BD/101060/2014, to F.B.); and CA17140 NANO2CLINIC COST Action.MDPIRepositório Científico do Instituto Nacional de SaúdeFernández-Bertólez, NataliaBrandão, FátimaCosta, CarlaPásaro, EduardoTeixeira, João PauloLaffon, BlancaValdiglesias, Vanessa2022-07-06T16:23:31Z2021-08-092021-08-09T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/8077engInt J Mol Sci. 2021 Aug 9;22(16):8558. doi: 10.3390/ijms221685581661-659610.3390/ijms22168558info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:42:24Zoai:repositorio.insa.pt:10400.18/8077Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:42:48.390857Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Suitability of the In Vitro Cytokinesis-Block Micronucleus Test for Genotoxicity Assessment of TiO2 Nanoparticles on SH-SY5Y Cells
title Suitability of the In Vitro Cytokinesis-Block Micronucleus Test for Genotoxicity Assessment of TiO2 Nanoparticles on SH-SY5Y Cells
spellingShingle Suitability of the In Vitro Cytokinesis-Block Micronucleus Test for Genotoxicity Assessment of TiO2 Nanoparticles on SH-SY5Y Cells
Fernández-Bertólez, Natalia
SH-SY5Y Cells
TiO2 Nanoparticles
Cytochalasin-B
Cytokinesis-Block Micronucleus Test
Flow Cytometry Micronucleus Test
Nanomaterials
Uptake
Genotoxicidade Ambiental
title_short Suitability of the In Vitro Cytokinesis-Block Micronucleus Test for Genotoxicity Assessment of TiO2 Nanoparticles on SH-SY5Y Cells
title_full Suitability of the In Vitro Cytokinesis-Block Micronucleus Test for Genotoxicity Assessment of TiO2 Nanoparticles on SH-SY5Y Cells
title_fullStr Suitability of the In Vitro Cytokinesis-Block Micronucleus Test for Genotoxicity Assessment of TiO2 Nanoparticles on SH-SY5Y Cells
title_full_unstemmed Suitability of the In Vitro Cytokinesis-Block Micronucleus Test for Genotoxicity Assessment of TiO2 Nanoparticles on SH-SY5Y Cells
title_sort Suitability of the In Vitro Cytokinesis-Block Micronucleus Test for Genotoxicity Assessment of TiO2 Nanoparticles on SH-SY5Y Cells
author Fernández-Bertólez, Natalia
author_facet Fernández-Bertólez, Natalia
Brandão, Fátima
Costa, Carla
Pásaro, Eduardo
Teixeira, João Paulo
Laffon, Blanca
Valdiglesias, Vanessa
author_role author
author2 Brandão, Fátima
Costa, Carla
Pásaro, Eduardo
Teixeira, João Paulo
Laffon, Blanca
Valdiglesias, Vanessa
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Nacional de Saúde
dc.contributor.author.fl_str_mv Fernández-Bertólez, Natalia
Brandão, Fátima
Costa, Carla
Pásaro, Eduardo
Teixeira, João Paulo
Laffon, Blanca
Valdiglesias, Vanessa
dc.subject.por.fl_str_mv SH-SY5Y Cells
TiO2 Nanoparticles
Cytochalasin-B
Cytokinesis-Block Micronucleus Test
Flow Cytometry Micronucleus Test
Nanomaterials
Uptake
Genotoxicidade Ambiental
topic SH-SY5Y Cells
TiO2 Nanoparticles
Cytochalasin-B
Cytokinesis-Block Micronucleus Test
Flow Cytometry Micronucleus Test
Nanomaterials
Uptake
Genotoxicidade Ambiental
description (This article belongs to the Special Issue Toxicity of Nanomaterials and Legacy Contaminants: Risks to the Environment and Human Health)
publishDate 2021
dc.date.none.fl_str_mv 2021-08-09
2021-08-09T00:00:00Z
2022-07-06T16:23:31Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.18/8077
url http://hdl.handle.net/10400.18/8077
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Int J Mol Sci. 2021 Aug 9;22(16):8558. doi: 10.3390/ijms22168558
1661-6596
10.3390/ijms22168558
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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