Comparative analysis of the bronchoalveolar microbiome in Portuguese patients with different chronic lung disorders
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10451/49111 |
Resumo: | © The Author(s) 2021. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
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Comparative analysis of the bronchoalveolar microbiome in Portuguese patients with different chronic lung disorders© The Author(s) 2021. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.The lung is inhabited by a diverse microbiome that originates from the oropharynx by a mechanism of micro-aspiration. Its bacterial biomass is usually low; however, this condition shifts in lung cancer (LC), chronic obstructive pulmonary disease (COPD) and interstitial lung disease (ILD). These chronic lung disorders (CLD) may coexist in the same patient as comorbidities and share common risk factors, among which the microbiome is included. We characterized the microbiome of 106 bronchoalveolar lavages. Samples were initially subdivided into cancer and non-cancer and high-throughput sequenced for the 16S rRNA gene. Additionally, we used a cohort of 25 CLD patients where crossed comorbidities were excluded. Firmicutes, Proteobacteria and Bacteroidetes were the most prevalent phyla independently of the analyzed group. Streptococcus and Prevotella were associated with LC and Haemophilus was enhanced in COPD versus ILD. Although no significant discrepancies in microbial diversity were observed between cancer and non-cancer samples, statistical tests suggested a gradient across CLD where COPD and ILD displayed the highest and lowest alpha diversities, respectively. Moreover, COPD and ILD were separated in two clusters by the unweighted UniFrac distance (P value = 0.0068). Our results support the association of Streptoccocus and Prevotella with LC and of Haemophilus with COPD, and advocate for specific CLD signatures.This work was supported by Norte Portugal Regional Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER)—project NORTE-01-0145-FEDER-000029. This work was also financed by FEDER funds through COMPETE 2020 (Operacional Programme for Competitiveness and Internationalization— POCI) and by private funds through the Young Investigator Prize Francisco Augusto da Fonseca Dias and Maria José Melenas da Fonseca Dias to P.I.M. FCT supports P.I.M. through a post-doctoral fellowship (SFRH/BPD/120777/2016), financed by the Portuguese State Budget of the Ministry for Science, Technology and High Education and from the European Social Fund (Programa Operacional do Capital Humano—POCH). M.P-L was partially supported by FCT under the Programa Operacional Potencial Humano—Quadro de Referência Estratégico Nacional funds from the European Social Fund and Portuguese State Budget of the Ministry for Science, Technology and High Education (IF/00764/2013; RX: IF/00359/2015).Springer NatureRepositório da Universidade de LisboaSeixas, SusanaKolbe, Allison R.Gomes, SílviaSucena, MariaSousa, CatarinaVaz Rodrigues, LuísTeixeira, GilbertoPinto, PaulaTavares de Abreu, TiagoBárbara, CristinaSemedo, JúlioMota, LeonorCarvalho, Ana SofiaMatthiesen, RuneMarques, Patrícia IsabelPérez-Losada, Marcos2021-07-26T12:43:52Z20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/49111engSci Rep. 2021 Jul 22;11(1):1504210.1038/s41598-021-94468-y2045-2322info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:52:43Zoai:repositorio.ul.pt:10451/49111Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:00:50.651990Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Comparative analysis of the bronchoalveolar microbiome in Portuguese patients with different chronic lung disorders |
| title |
Comparative analysis of the bronchoalveolar microbiome in Portuguese patients with different chronic lung disorders |
| spellingShingle |
Comparative analysis of the bronchoalveolar microbiome in Portuguese patients with different chronic lung disorders Seixas, Susana |
| title_short |
Comparative analysis of the bronchoalveolar microbiome in Portuguese patients with different chronic lung disorders |
| title_full |
Comparative analysis of the bronchoalveolar microbiome in Portuguese patients with different chronic lung disorders |
| title_fullStr |
Comparative analysis of the bronchoalveolar microbiome in Portuguese patients with different chronic lung disorders |
| title_full_unstemmed |
Comparative analysis of the bronchoalveolar microbiome in Portuguese patients with different chronic lung disorders |
| title_sort |
Comparative analysis of the bronchoalveolar microbiome in Portuguese patients with different chronic lung disorders |
| author |
Seixas, Susana |
| author_facet |
Seixas, Susana Kolbe, Allison R. Gomes, Sílvia Sucena, Maria Sousa, Catarina Vaz Rodrigues, Luís Teixeira, Gilberto Pinto, Paula Tavares de Abreu, Tiago Bárbara, Cristina Semedo, Júlio Mota, Leonor Carvalho, Ana Sofia Matthiesen, Rune Marques, Patrícia Isabel Pérez-Losada, Marcos |
| author_role |
author |
| author2 |
Kolbe, Allison R. Gomes, Sílvia Sucena, Maria Sousa, Catarina Vaz Rodrigues, Luís Teixeira, Gilberto Pinto, Paula Tavares de Abreu, Tiago Bárbara, Cristina Semedo, Júlio Mota, Leonor Carvalho, Ana Sofia Matthiesen, Rune Marques, Patrícia Isabel Pérez-Losada, Marcos |
| author2_role |
author author author author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
| dc.contributor.author.fl_str_mv |
Seixas, Susana Kolbe, Allison R. Gomes, Sílvia Sucena, Maria Sousa, Catarina Vaz Rodrigues, Luís Teixeira, Gilberto Pinto, Paula Tavares de Abreu, Tiago Bárbara, Cristina Semedo, Júlio Mota, Leonor Carvalho, Ana Sofia Matthiesen, Rune Marques, Patrícia Isabel Pérez-Losada, Marcos |
| description |
© The Author(s) 2021. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021-07-26T12:43:52Z 2021 2021-01-01T00:00:00Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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http://hdl.handle.net/10451/49111 |
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http://hdl.handle.net/10451/49111 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
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Sci Rep. 2021 Jul 22;11(1):15042 10.1038/s41598-021-94468-y 2045-2322 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Springer Nature |
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Springer Nature |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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