A novelty system for biotization of plant microshoots and collection of natural compounds
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.13/4042 |
Resumo: | An in vitro plant microshoot culture system composed of two phases; a liquid phase overlaid by a floating solid phase, which is described in detail herein. This system is designed to enable the extraction of natural compounds released/disseminated into the liquid phase during root growth, thus facilitating their processing and biochemical characterization. The solid phase holds the plant afloat and enables the simultaneous culture of a microorganism, yet avoiding its penetration into the liquid phase, where the roots are submerged. Both phases can be independently formulated as required for growth optimization of both organisms. Considering the closed system and known variables described in this patent, applications of the described method include testing with pesticides, herbicides, and other similar products. |
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A novelty system for biotization of plant microshoots and collection of natural compoundsBiotechnologyBiotizationChemical analysisDouble-phase mediumPhenolic compoundsLiquid chromatographyDiode array detectorMass spectrometry.Escola Superior de Tecnologias e GestãoAn in vitro plant microshoot culture system composed of two phases; a liquid phase overlaid by a floating solid phase, which is described in detail herein. This system is designed to enable the extraction of natural compounds released/disseminated into the liquid phase during root growth, thus facilitating their processing and biochemical characterization. The solid phase holds the plant afloat and enables the simultaneous culture of a microorganism, yet avoiding its penetration into the liquid phase, where the roots are submerged. Both phases can be independently formulated as required for growth optimization of both organisms. Considering the closed system and known variables described in this patent, applications of the described method include testing with pesticides, herbicides, and other similar products.MDPIDigitUMaCastro, Mário Rui da Costa Basílio eRagonezi, CarlaOliveira, Paulo Guilherme Leandro deZavattieri, Maria Amely2022-02-04T11:50:25Z2019-01-01T00:00:00Z2019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.13/4042engCastro, M., Ragonezi, C., de Oliveira, P., & Zavattieri, M. (2019). A novelty system for biotization of plant microshoots and collection of natural compounds. Methods and Protocols, 2(1). http://dx.doi.org/10.3390/mps201000510.3390/mps2010005info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-09-05T12:57:10Zoai:digituma.uma.pt:10400.13/4042Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:07:43.685048Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
A novelty system for biotization of plant microshoots and collection of natural compounds |
title |
A novelty system for biotization of plant microshoots and collection of natural compounds |
spellingShingle |
A novelty system for biotization of plant microshoots and collection of natural compounds Castro, Mário Rui da Costa Basílio e Biotechnology Biotization Chemical analysis Double-phase medium Phenolic compounds Liquid chromatography Diode array detector Mass spectrometry . Escola Superior de Tecnologias e Gestão |
title_short |
A novelty system for biotization of plant microshoots and collection of natural compounds |
title_full |
A novelty system for biotization of plant microshoots and collection of natural compounds |
title_fullStr |
A novelty system for biotization of plant microshoots and collection of natural compounds |
title_full_unstemmed |
A novelty system for biotization of plant microshoots and collection of natural compounds |
title_sort |
A novelty system for biotization of plant microshoots and collection of natural compounds |
author |
Castro, Mário Rui da Costa Basílio e |
author_facet |
Castro, Mário Rui da Costa Basílio e Ragonezi, Carla Oliveira, Paulo Guilherme Leandro de Zavattieri, Maria Amely |
author_role |
author |
author2 |
Ragonezi, Carla Oliveira, Paulo Guilherme Leandro de Zavattieri, Maria Amely |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
DigitUMa |
dc.contributor.author.fl_str_mv |
Castro, Mário Rui da Costa Basílio e Ragonezi, Carla Oliveira, Paulo Guilherme Leandro de Zavattieri, Maria Amely |
dc.subject.por.fl_str_mv |
Biotechnology Biotization Chemical analysis Double-phase medium Phenolic compounds Liquid chromatography Diode array detector Mass spectrometry . Escola Superior de Tecnologias e Gestão |
topic |
Biotechnology Biotization Chemical analysis Double-phase medium Phenolic compounds Liquid chromatography Diode array detector Mass spectrometry . Escola Superior de Tecnologias e Gestão |
description |
An in vitro plant microshoot culture system composed of two phases; a liquid phase overlaid by a floating solid phase, which is described in detail herein. This system is designed to enable the extraction of natural compounds released/disseminated into the liquid phase during root growth, thus facilitating their processing and biochemical characterization. The solid phase holds the plant afloat and enables the simultaneous culture of a microorganism, yet avoiding its penetration into the liquid phase, where the roots are submerged. Both phases can be independently formulated as required for growth optimization of both organisms. Considering the closed system and known variables described in this patent, applications of the described method include testing with pesticides, herbicides, and other similar products. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-01-01T00:00:00Z 2019-01-01T00:00:00Z 2022-02-04T11:50:25Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.13/4042 |
url |
http://hdl.handle.net/10400.13/4042 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Castro, M., Ragonezi, C., de Oliveira, P., & Zavattieri, M. (2019). A novelty system for biotization of plant microshoots and collection of natural compounds. Methods and Protocols, 2(1). http://dx.doi.org/10.3390/mps2010005 10.3390/mps2010005 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799129945576833024 |