A novelty system for biotization of plant microshoots and collection of natural compounds

Detalhes bibliográficos
Autor(a) principal: Castro, Mário Rui da Costa Basílio e
Data de Publicação: 2019
Outros Autores: Ragonezi, Carla, Oliveira, Paulo Guilherme Leandro de, Zavattieri, Maria Amely
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.13/4042
Resumo: An in vitro plant microshoot culture system composed of two phases; a liquid phase overlaid by a floating solid phase, which is described in detail herein. This system is designed to enable the extraction of natural compounds released/disseminated into the liquid phase during root growth, thus facilitating their processing and biochemical characterization. The solid phase holds the plant afloat and enables the simultaneous culture of a microorganism, yet avoiding its penetration into the liquid phase, where the roots are submerged. Both phases can be independently formulated as required for growth optimization of both organisms. Considering the closed system and known variables described in this patent, applications of the described method include testing with pesticides, herbicides, and other similar products.
id RCAP_899d6d3d821c2c6c9a84b76f9aa1c990
oai_identifier_str oai:digituma.uma.pt:10400.13/4042
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling A novelty system for biotization of plant microshoots and collection of natural compoundsBiotechnologyBiotizationChemical analysisDouble-phase mediumPhenolic compoundsLiquid chromatographyDiode array detectorMass spectrometry.Escola Superior de Tecnologias e GestãoAn in vitro plant microshoot culture system composed of two phases; a liquid phase overlaid by a floating solid phase, which is described in detail herein. This system is designed to enable the extraction of natural compounds released/disseminated into the liquid phase during root growth, thus facilitating their processing and biochemical characterization. The solid phase holds the plant afloat and enables the simultaneous culture of a microorganism, yet avoiding its penetration into the liquid phase, where the roots are submerged. Both phases can be independently formulated as required for growth optimization of both organisms. Considering the closed system and known variables described in this patent, applications of the described method include testing with pesticides, herbicides, and other similar products.MDPIDigitUMaCastro, Mário Rui da Costa Basílio eRagonezi, CarlaOliveira, Paulo Guilherme Leandro deZavattieri, Maria Amely2022-02-04T11:50:25Z2019-01-01T00:00:00Z2019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.13/4042engCastro, M., Ragonezi, C., de Oliveira, P., & Zavattieri, M. (2019). A novelty system for biotization of plant microshoots and collection of natural compounds. Methods and Protocols, 2(1). http://dx.doi.org/10.3390/mps201000510.3390/mps2010005info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-09-05T12:57:10Zoai:digituma.uma.pt:10400.13/4042Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:07:43.685048Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv A novelty system for biotization of plant microshoots and collection of natural compounds
title A novelty system for biotization of plant microshoots and collection of natural compounds
spellingShingle A novelty system for biotization of plant microshoots and collection of natural compounds
Castro, Mário Rui da Costa Basílio e
Biotechnology
Biotization
Chemical analysis
Double-phase medium
Phenolic compounds
Liquid chromatography
Diode array detector
Mass spectrometry
.
Escola Superior de Tecnologias e Gestão
title_short A novelty system for biotization of plant microshoots and collection of natural compounds
title_full A novelty system for biotization of plant microshoots and collection of natural compounds
title_fullStr A novelty system for biotization of plant microshoots and collection of natural compounds
title_full_unstemmed A novelty system for biotization of plant microshoots and collection of natural compounds
title_sort A novelty system for biotization of plant microshoots and collection of natural compounds
author Castro, Mário Rui da Costa Basílio e
author_facet Castro, Mário Rui da Costa Basílio e
Ragonezi, Carla
Oliveira, Paulo Guilherme Leandro de
Zavattieri, Maria Amely
author_role author
author2 Ragonezi, Carla
Oliveira, Paulo Guilherme Leandro de
Zavattieri, Maria Amely
author2_role author
author
author
dc.contributor.none.fl_str_mv DigitUMa
dc.contributor.author.fl_str_mv Castro, Mário Rui da Costa Basílio e
Ragonezi, Carla
Oliveira, Paulo Guilherme Leandro de
Zavattieri, Maria Amely
dc.subject.por.fl_str_mv Biotechnology
Biotization
Chemical analysis
Double-phase medium
Phenolic compounds
Liquid chromatography
Diode array detector
Mass spectrometry
.
Escola Superior de Tecnologias e Gestão
topic Biotechnology
Biotization
Chemical analysis
Double-phase medium
Phenolic compounds
Liquid chromatography
Diode array detector
Mass spectrometry
.
Escola Superior de Tecnologias e Gestão
description An in vitro plant microshoot culture system composed of two phases; a liquid phase overlaid by a floating solid phase, which is described in detail herein. This system is designed to enable the extraction of natural compounds released/disseminated into the liquid phase during root growth, thus facilitating their processing and biochemical characterization. The solid phase holds the plant afloat and enables the simultaneous culture of a microorganism, yet avoiding its penetration into the liquid phase, where the roots are submerged. Both phases can be independently formulated as required for growth optimization of both organisms. Considering the closed system and known variables described in this patent, applications of the described method include testing with pesticides, herbicides, and other similar products.
publishDate 2019
dc.date.none.fl_str_mv 2019-01-01T00:00:00Z
2019-01-01T00:00:00Z
2022-02-04T11:50:25Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.13/4042
url http://hdl.handle.net/10400.13/4042
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Castro, M., Ragonezi, C., de Oliveira, P., & Zavattieri, M. (2019). A novelty system for biotization of plant microshoots and collection of natural compounds. Methods and Protocols, 2(1). http://dx.doi.org/10.3390/mps2010005
10.3390/mps2010005
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799129945576833024