A bi-layer electrospun nanofiltration membrane for plasmid DNA recovery from fermentation broths
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/26707 |
Resumo: | The demanding ever-increasing quantities of highly purified biomolecules by bio-industries, has triggered the development of new, more efficient, purification techniques. The application of membrane-based technologies has become very attractive in this field, for their high throughput capability, simplicity of operation and scale-up. Herein we report the production of a bi-layer membrane by electrospinning (ES), in which a support of poly ε-caprolactone nanofibers was coated with a polyethylene oxide/sodium alginate layer, and subsequently cross-linked with calcium chloride. The membranes were characterized by SEM, ATR-FTIR, contact angle measurements, and were applied in the recovery process of a plasmid. The results show that membranes retained the suspended solids while allowing the permeation of plasmid DNA, with high recovery yields and improved RNA retention. Moreover, they also showed a very low fouling tendency. To the best of our knowledge it is the first time that ES membranes are applied in this type of bioprocess. |
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A bi-layer electrospun nanofiltration membrane for plasmid DNA recovery from fermentation brothsMicrofiltrationElectrospinningBi-layer membraneLysatePlasmid DNADNAPlasmidScience & TechnologyThe demanding ever-increasing quantities of highly purified biomolecules by bio-industries, has triggered the development of new, more efficient, purification techniques. The application of membrane-based technologies has become very attractive in this field, for their high throughput capability, simplicity of operation and scale-up. Herein we report the production of a bi-layer membrane by electrospinning (ES), in which a support of poly ε-caprolactone nanofibers was coated with a polyethylene oxide/sodium alginate layer, and subsequently cross-linked with calcium chloride. The membranes were characterized by SEM, ATR-FTIR, contact angle measurements, and were applied in the recovery process of a plasmid. The results show that membranes retained the suspended solids while allowing the permeation of plasmid DNA, with high recovery yields and improved RNA retention. Moreover, they also showed a very low fouling tendency. To the best of our knowledge it is the first time that ES membranes are applied in this type of bioprocess.This work was supported by the Portuguese Foundation for Science and Technology (FCT), (PTDC/EME-TME/103375/2008 and PTDC/EBB-BIO/114320/2009). To Ricardo Fradique for helping in the production of the graphical abstract.ElsevierUniversidade do MinhoCorreia, Tiago R.Antunes, Bernardo P.Castilho, Pedro H.Nunes, José C.Amorim, M. T. Pessoa deEscobar, I. C.Correia, I. J.Morão, A.20132013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/26707eng1383-586610.1016/j.seppur.2013.03.049http://dx.doi.org/10.1016/j.seppur.2013.03.049info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:48:48ZPortal AgregadorONG |
dc.title.none.fl_str_mv |
A bi-layer electrospun nanofiltration membrane for plasmid DNA recovery from fermentation broths |
title |
A bi-layer electrospun nanofiltration membrane for plasmid DNA recovery from fermentation broths |
spellingShingle |
A bi-layer electrospun nanofiltration membrane for plasmid DNA recovery from fermentation broths Correia, Tiago R. Microfiltration Electrospinning Bi-layer membrane Lysate Plasmid DNA DNA Plasmid Science & Technology |
title_short |
A bi-layer electrospun nanofiltration membrane for plasmid DNA recovery from fermentation broths |
title_full |
A bi-layer electrospun nanofiltration membrane for plasmid DNA recovery from fermentation broths |
title_fullStr |
A bi-layer electrospun nanofiltration membrane for plasmid DNA recovery from fermentation broths |
title_full_unstemmed |
A bi-layer electrospun nanofiltration membrane for plasmid DNA recovery from fermentation broths |
title_sort |
A bi-layer electrospun nanofiltration membrane for plasmid DNA recovery from fermentation broths |
author |
Correia, Tiago R. |
author_facet |
Correia, Tiago R. Antunes, Bernardo P. Castilho, Pedro H. Nunes, José C. Amorim, M. T. Pessoa de Escobar, I. C. Correia, I. J. Morão, A. |
author_role |
author |
author2 |
Antunes, Bernardo P. Castilho, Pedro H. Nunes, José C. Amorim, M. T. Pessoa de Escobar, I. C. Correia, I. J. Morão, A. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Correia, Tiago R. Antunes, Bernardo P. Castilho, Pedro H. Nunes, José C. Amorim, M. T. Pessoa de Escobar, I. C. Correia, I. J. Morão, A. |
dc.subject.por.fl_str_mv |
Microfiltration Electrospinning Bi-layer membrane Lysate Plasmid DNA DNA Plasmid Science & Technology |
topic |
Microfiltration Electrospinning Bi-layer membrane Lysate Plasmid DNA DNA Plasmid Science & Technology |
description |
The demanding ever-increasing quantities of highly purified biomolecules by bio-industries, has triggered the development of new, more efficient, purification techniques. The application of membrane-based technologies has become very attractive in this field, for their high throughput capability, simplicity of operation and scale-up. Herein we report the production of a bi-layer membrane by electrospinning (ES), in which a support of poly ε-caprolactone nanofibers was coated with a polyethylene oxide/sodium alginate layer, and subsequently cross-linked with calcium chloride. The membranes were characterized by SEM, ATR-FTIR, contact angle measurements, and were applied in the recovery process of a plasmid. The results show that membranes retained the suspended solids while allowing the permeation of plasmid DNA, with high recovery yields and improved RNA retention. Moreover, they also showed a very low fouling tendency. To the best of our knowledge it is the first time that ES membranes are applied in this type of bioprocess. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013 2013-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/26707 |
url |
http://hdl.handle.net/1822/26707 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1383-5866 10.1016/j.seppur.2013.03.049 http://dx.doi.org/10.1016/j.seppur.2013.03.049 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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repository.mail.fl_str_mv |
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1777303849740009472 |