Exploring the denaturation of whey proteins upon application of moderate electric fields : a kinetic and thermodynamic study
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/16727 |
Resumo: | Thermal processing often results in disruption of the native conformation of whey proteins, thus affecting functional properties. The aim of this work was to evaluate the effects of moderate electric fields on denaturation kinetics and thermodynamic properties of whey protein dispersions at temperatures ranging from 75 to 90 °C. Application of electric fields led to a lower denaturation of whey proteins, kinetically traduced by lower values of reaction order (n) and rate constant (k) (p < 0.05), when compared to those from conventional heating under equivalent heating rates and holding times. Furthermore, the application of electric fields combined with short come-up times has reduced considerably the denaturation of proteins during early stages of heating (>30% of native soluble protein than conventional heating) and has determined also considerable changes in calculated thermodynamic properties (such as Ea, ΔH, ΔS). In general, denaturation reactions during moderate electric fields processing were less dependent on temperature increase. |
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Exploring the denaturation of whey proteins upon application of moderate electric fields : a kinetic and thermodynamic studyWhey proteinsoOhmic heatingDenaturationThermodynamic propertieswhey proteinsScience & TechnologyThermal processing often results in disruption of the native conformation of whey proteins, thus affecting functional properties. The aim of this work was to evaluate the effects of moderate electric fields on denaturation kinetics and thermodynamic properties of whey protein dispersions at temperatures ranging from 75 to 90 °C. Application of electric fields led to a lower denaturation of whey proteins, kinetically traduced by lower values of reaction order (n) and rate constant (k) (p < 0.05), when compared to those from conventional heating under equivalent heating rates and holding times. Furthermore, the application of electric fields combined with short come-up times has reduced considerably the denaturation of proteins during early stages of heating (>30% of native soluble protein than conventional heating) and has determined also considerable changes in calculated thermodynamic properties (such as Ea, ΔH, ΔS). In general, denaturation reactions during moderate electric fields processing were less dependent on temperature increase.RNP gratefully acknowledges a doctoral grant (SFRH/BD/31635/2006) from the Fundacao para Ciencia e Tecnologia (FCT, Portugal)American Chemical SocietyUniversidade do MinhoPereira, RicardoTeixeira, J. A.Vicente, A. A.2011-07-042011-07-04T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/16727eng0021-856110.1021/jf201727s21932854info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:38:05Zoai:repositorium.sdum.uminho.pt:1822/16727Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:34:28.080187Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Exploring the denaturation of whey proteins upon application of moderate electric fields : a kinetic and thermodynamic study |
title |
Exploring the denaturation of whey proteins upon application of moderate electric fields : a kinetic and thermodynamic study |
spellingShingle |
Exploring the denaturation of whey proteins upon application of moderate electric fields : a kinetic and thermodynamic study Pereira, Ricardo Whey proteinso Ohmic heating Denaturation Thermodynamic properties whey proteins Science & Technology |
title_short |
Exploring the denaturation of whey proteins upon application of moderate electric fields : a kinetic and thermodynamic study |
title_full |
Exploring the denaturation of whey proteins upon application of moderate electric fields : a kinetic and thermodynamic study |
title_fullStr |
Exploring the denaturation of whey proteins upon application of moderate electric fields : a kinetic and thermodynamic study |
title_full_unstemmed |
Exploring the denaturation of whey proteins upon application of moderate electric fields : a kinetic and thermodynamic study |
title_sort |
Exploring the denaturation of whey proteins upon application of moderate electric fields : a kinetic and thermodynamic study |
author |
Pereira, Ricardo |
author_facet |
Pereira, Ricardo Teixeira, J. A. Vicente, A. A. |
author_role |
author |
author2 |
Teixeira, J. A. Vicente, A. A. |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Pereira, Ricardo Teixeira, J. A. Vicente, A. A. |
dc.subject.por.fl_str_mv |
Whey proteinso Ohmic heating Denaturation Thermodynamic properties whey proteins Science & Technology |
topic |
Whey proteinso Ohmic heating Denaturation Thermodynamic properties whey proteins Science & Technology |
description |
Thermal processing often results in disruption of the native conformation of whey proteins, thus affecting functional properties. The aim of this work was to evaluate the effects of moderate electric fields on denaturation kinetics and thermodynamic properties of whey protein dispersions at temperatures ranging from 75 to 90 °C. Application of electric fields led to a lower denaturation of whey proteins, kinetically traduced by lower values of reaction order (n) and rate constant (k) (p < 0.05), when compared to those from conventional heating under equivalent heating rates and holding times. Furthermore, the application of electric fields combined with short come-up times has reduced considerably the denaturation of proteins during early stages of heating (>30% of native soluble protein than conventional heating) and has determined also considerable changes in calculated thermodynamic properties (such as Ea, ΔH, ΔS). In general, denaturation reactions during moderate electric fields processing were less dependent on temperature increase. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-07-04 2011-07-04T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/16727 |
url |
http://hdl.handle.net/1822/16727 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0021-8561 10.1021/jf201727s 21932854 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
American Chemical Society |
publisher.none.fl_str_mv |
American Chemical Society |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132866535227392 |