Transcriptional profiling of HOXA9-regulated genes in human glioblastoma cell models
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/35778 |
Resumo: | The data here described pertain to the article by Pojo et al. (2015) titled A transcriptomic signature mediated by HOXA9 promotes human glioblastoma initiation, aggressiveness and resistance to temozolomide (Pojo, Goncalves et al. 2015). HOX genes are part of the homeobox genes family, which encodes transcription factors crucial during embryonic development [6] and [9] and also in postdevelopmental regulation [8], [14], [13] and [7]. Alterations interfering with the regulation of these genes may lead to tumorigenesis in adults. Due to their contributions in the control of important cellular processes, the deregulation of HOX genes are ultimately correlated with cancer treatment failure and patients poor prognosis ( [5] and [1]; Costa, Smith et al. 2010; Pojo, Goncalves et al. 2015). Recently, our studies showed that HOXA9 overexpression is associated with poor prognosis in patients with glioblastoma (GBM), the most common and most malignant primary brain tumor. Mechanistically, HOXA9 is associated with resistance to chemotherapy and with pro-proliferative, pro-invasive and anti-apoptotic features (Costa, Smith et al. 2010; Pojo, Goncalves et al. 2015) in GBM in vitro models. Since HOXA9 is a transcription factor, its target genes can be the true biological effectors of its aggressiveness. In this context, whole genome Agilents microarrays were used to obtain the full transcriptome of HOXA9 in a variety of GBM cell models, including human immortalized astrocytes, established GBM cell lines, and GBM patient-derived cell cultures. Here, we provide detailed methods, including experimental design and microarray data analyses, which can be accessed in Gene Expression Omnibus (GEO) under the accession number GSE56517. Additional interpretation of the data is included and supplemented in (Pojo, Goncalves et al. 2015). |
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Transcriptional profiling of HOXA9-regulated genes in human glioblastoma cell modelsHOXA9TranscriptomeGBMMicroarraysR/bioconductorScience & TechnologyThe data here described pertain to the article by Pojo et al. (2015) titled A transcriptomic signature mediated by HOXA9 promotes human glioblastoma initiation, aggressiveness and resistance to temozolomide (Pojo, Goncalves et al. 2015). HOX genes are part of the homeobox genes family, which encodes transcription factors crucial during embryonic development [6] and [9] and also in postdevelopmental regulation [8], [14], [13] and [7]. Alterations interfering with the regulation of these genes may lead to tumorigenesis in adults. Due to their contributions in the control of important cellular processes, the deregulation of HOX genes are ultimately correlated with cancer treatment failure and patients poor prognosis ( [5] and [1]; Costa, Smith et al. 2010; Pojo, Goncalves et al. 2015). Recently, our studies showed that HOXA9 overexpression is associated with poor prognosis in patients with glioblastoma (GBM), the most common and most malignant primary brain tumor. Mechanistically, HOXA9 is associated with resistance to chemotherapy and with pro-proliferative, pro-invasive and anti-apoptotic features (Costa, Smith et al. 2010; Pojo, Goncalves et al. 2015) in GBM in vitro models. Since HOXA9 is a transcription factor, its target genes can be the true biological effectors of its aggressiveness. In this context, whole genome Agilents microarrays were used to obtain the full transcriptome of HOXA9 in a variety of GBM cell models, including human immortalized astrocytes, established GBM cell lines, and GBM patient-derived cell cultures. Here, we provide detailed methods, including experimental design and microarray data analyses, which can be accessed in Gene Expression Omnibus (GEO) under the accession number GSE56517. Additional interpretation of the data is included and supplemented in (Pojo, Goncalves et al. 2015).Elsevier Inc.Universidade do MinhoGonçalves, Céline S.Magalhães, Ana XavierPojo, MartaOliveira, Ana IsabelReis, R. M.Sousa, NunoRocha, MiguelCosta, Bruno Marques20152015-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/35778engGonçalves, Céline S.; Xavier-Magalhães, Ana; Pojo, Marta; Oliveira, Ana Isabel; ; Reis, Rui M.; Sousa, Nuno; Rocha, Miguel; Costa, Bruno M., Transcriptional profiling of HOXA9-regulated genes in human glioblastoma cell models. Genomics Data, 5, 54-58, 20152213-596010.1016/j.gdata.2015.05.010http://www.journals.elsevier.com/genomics-datainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:27:42Zoai:repositorium.sdum.uminho.pt:1822/35778Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:22:24.863439Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Transcriptional profiling of HOXA9-regulated genes in human glioblastoma cell models |
title |
Transcriptional profiling of HOXA9-regulated genes in human glioblastoma cell models |
spellingShingle |
Transcriptional profiling of HOXA9-regulated genes in human glioblastoma cell models Gonçalves, Céline S. HOXA9 Transcriptome GBM Microarrays R/bioconductor Science & Technology |
title_short |
Transcriptional profiling of HOXA9-regulated genes in human glioblastoma cell models |
title_full |
Transcriptional profiling of HOXA9-regulated genes in human glioblastoma cell models |
title_fullStr |
Transcriptional profiling of HOXA9-regulated genes in human glioblastoma cell models |
title_full_unstemmed |
Transcriptional profiling of HOXA9-regulated genes in human glioblastoma cell models |
title_sort |
Transcriptional profiling of HOXA9-regulated genes in human glioblastoma cell models |
author |
Gonçalves, Céline S. |
author_facet |
Gonçalves, Céline S. Magalhães, Ana Xavier Pojo, Marta Oliveira, Ana Isabel Reis, R. M. Sousa, Nuno Rocha, Miguel Costa, Bruno Marques |
author_role |
author |
author2 |
Magalhães, Ana Xavier Pojo, Marta Oliveira, Ana Isabel Reis, R. M. Sousa, Nuno Rocha, Miguel Costa, Bruno Marques |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Gonçalves, Céline S. Magalhães, Ana Xavier Pojo, Marta Oliveira, Ana Isabel Reis, R. M. Sousa, Nuno Rocha, Miguel Costa, Bruno Marques |
dc.subject.por.fl_str_mv |
HOXA9 Transcriptome GBM Microarrays R/bioconductor Science & Technology |
topic |
HOXA9 Transcriptome GBM Microarrays R/bioconductor Science & Technology |
description |
The data here described pertain to the article by Pojo et al. (2015) titled A transcriptomic signature mediated by HOXA9 promotes human glioblastoma initiation, aggressiveness and resistance to temozolomide (Pojo, Goncalves et al. 2015). HOX genes are part of the homeobox genes family, which encodes transcription factors crucial during embryonic development [6] and [9] and also in postdevelopmental regulation [8], [14], [13] and [7]. Alterations interfering with the regulation of these genes may lead to tumorigenesis in adults. Due to their contributions in the control of important cellular processes, the deregulation of HOX genes are ultimately correlated with cancer treatment failure and patients poor prognosis ( [5] and [1]; Costa, Smith et al. 2010; Pojo, Goncalves et al. 2015). Recently, our studies showed that HOXA9 overexpression is associated with poor prognosis in patients with glioblastoma (GBM), the most common and most malignant primary brain tumor. Mechanistically, HOXA9 is associated with resistance to chemotherapy and with pro-proliferative, pro-invasive and anti-apoptotic features (Costa, Smith et al. 2010; Pojo, Goncalves et al. 2015) in GBM in vitro models. Since HOXA9 is a transcription factor, its target genes can be the true biological effectors of its aggressiveness. In this context, whole genome Agilents microarrays were used to obtain the full transcriptome of HOXA9 in a variety of GBM cell models, including human immortalized astrocytes, established GBM cell lines, and GBM patient-derived cell cultures. Here, we provide detailed methods, including experimental design and microarray data analyses, which can be accessed in Gene Expression Omnibus (GEO) under the accession number GSE56517. Additional interpretation of the data is included and supplemented in (Pojo, Goncalves et al. 2015). |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015 2015-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/35778 |
url |
http://hdl.handle.net/1822/35778 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Gonçalves, Céline S.; Xavier-Magalhães, Ana; Pojo, Marta; Oliveira, Ana Isabel; ; Reis, Rui M.; Sousa, Nuno; Rocha, Miguel; Costa, Bruno M., Transcriptional profiling of HOXA9-regulated genes in human glioblastoma cell models. Genomics Data, 5, 54-58, 2015 2213-5960 10.1016/j.gdata.2015.05.010 http://www.journals.elsevier.com/genomics-data |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier Inc. |
publisher.none.fl_str_mv |
Elsevier Inc. |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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