Prolonged exposure of Stenotrophomonas maltophilia biofilms to trace levels of clofibric acid alters antimicrobial tolerance and virulence
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.18/6626 |
Resumo: | The presence of pharmaceuticals in water sources, including in drinking water (DW), is increasingly being recognized as an emerging and global concern for the environment and public health. Based on the principles of the "One Health" initiative, the present work aims to understand the effects of clofibric acid (CA), a lipid regulator, on the behavior of a selected bacterium isolated from drinking water (DW). Biofilms of the opportunistic pathogen Stenotrophomonas maltophilia were exposed to CA for 12 weeks at 170 and 17000 ng/L. The effects of CA were evaluated on planktonic S. maltophilia susceptibility to chlorine and antibiotics (amoxicillin, ciprofloxacin, erythromycin, kanamycin, levofloxacin, oxacillin, spectinomycin, tetracycline and trimethoprim-sulfamethoxazole), biofilm formation, motility, siderophores production and on the adhesion and internalization of the human colon adenocarcinoma cell line (HT-29). It was found that CA did not affect planktonic S. maltophilia tolerance to chlorine exposure. Additionally, no effects were observed on biofilm formation, motility and siderophores production. However, biofilms formed after CA exposure were more tolerant to chlorine disinfection and lower CFU reductions were obtained. Of additional concern was the effect of CA exposure on S. maltophilia increased tolerance to erythromycin. CA exposure also slightly reduced S. maltophilia ability to invade HT-29 cells. In conclusion, this work reinforces the importance of studying the effects of non-antibiotic contaminants on the behavior of environmental microorganisms, particularly their role as drivers affecting resistance evolution and selection. |
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Prolonged exposure of Stenotrophomonas maltophilia biofilms to trace levels of clofibric acid alters antimicrobial tolerance and virulenceAnti-Bacterial AgentsAnti-Infective AgentsBiofilmsCiprofloxacinClofibric AcidDrinking WaterDrug ToleranceHT29 CellsHumansStenotrophomonas maltophiliaVirulenceÁgua e SoloThe presence of pharmaceuticals in water sources, including in drinking water (DW), is increasingly being recognized as an emerging and global concern for the environment and public health. Based on the principles of the "One Health" initiative, the present work aims to understand the effects of clofibric acid (CA), a lipid regulator, on the behavior of a selected bacterium isolated from drinking water (DW). Biofilms of the opportunistic pathogen Stenotrophomonas maltophilia were exposed to CA for 12 weeks at 170 and 17000 ng/L. The effects of CA were evaluated on planktonic S. maltophilia susceptibility to chlorine and antibiotics (amoxicillin, ciprofloxacin, erythromycin, kanamycin, levofloxacin, oxacillin, spectinomycin, tetracycline and trimethoprim-sulfamethoxazole), biofilm formation, motility, siderophores production and on the adhesion and internalization of the human colon adenocarcinoma cell line (HT-29). It was found that CA did not affect planktonic S. maltophilia tolerance to chlorine exposure. Additionally, no effects were observed on biofilm formation, motility and siderophores production. However, biofilms formed after CA exposure were more tolerant to chlorine disinfection and lower CFU reductions were obtained. Of additional concern was the effect of CA exposure on S. maltophilia increased tolerance to erythromycin. CA exposure also slightly reduced S. maltophilia ability to invade HT-29 cells. In conclusion, this work reinforces the importance of studying the effects of non-antibiotic contaminants on the behavior of environmental microorganisms, particularly their role as drivers affecting resistance evolution and selection.Highlights: Non-antibiotic drugs, including clofibric acid (CA) can affect bacterial behavior; The presence of CA in water increased S. maltophilia tolerance to erythromycin; CA exposure increased biofilm tolerance to chorine; CA exposure decreased S. maltophilia ability to adhere and internalize HT-29 cells.This work was financially supported by: project UID/EQU/ 00511/2019 - Laboratory for Process Engineering, Environment, Biotechnology and Energy e LEPABE funded by national funds through FCT/MCTES (PIDDAC); Projects POCI-01-0145-FEDER- 030219, POCI-01-0145-FEDER-028397 and POCI-01-0247-FEDER- 035234 funded by FEDER funds through COMPETE2020 e Programa Operacional Competitividade e Internacionalizaç~ao (POCI) and by national funds (PIDDAC) through FCT/MCTES; Project “LEPABE-2-ECO-INNOVATION” e NORTE-01-0145-FEDER-000005, funded by Norte Portugal Regional Operational Programme (NORTE, 2020), under PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF). Grants attributed by Portuguese Foundation for Science and Technology e FCT e to In^es Gomes (SFRH/BD/103810/2014) and to Micaela Machado Querido (SFRH/BD/130203/2017).Elsevier/ PergamonRepositório Científico do Instituto Nacional de SaúdeGomes, I.B.Querido, M.M.Teixeira, J.P.Pereira, C.C.Simões, L.C.Simões, M.2020-05-09T11:07:46Z2019-112019-11-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/6626engChemosphere. 2019 Nov;235:327-335. doi: 10.1016/j.chemosphere.2019.06.184. Epub 2019 Jun 250045-653510.1016/j.chemosphere.2019.06.184info:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:41:46Zoai:repositorio.insa.pt:10400.18/6626Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:41:42.805949Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Prolonged exposure of Stenotrophomonas maltophilia biofilms to trace levels of clofibric acid alters antimicrobial tolerance and virulence |
title |
Prolonged exposure of Stenotrophomonas maltophilia biofilms to trace levels of clofibric acid alters antimicrobial tolerance and virulence |
spellingShingle |
Prolonged exposure of Stenotrophomonas maltophilia biofilms to trace levels of clofibric acid alters antimicrobial tolerance and virulence Gomes, I.B. Anti-Bacterial Agents Anti-Infective Agents Biofilms Ciprofloxacin Clofibric Acid Drinking Water Drug Tolerance HT29 Cells Humans Stenotrophomonas maltophilia Virulence Água e Solo |
title_short |
Prolonged exposure of Stenotrophomonas maltophilia biofilms to trace levels of clofibric acid alters antimicrobial tolerance and virulence |
title_full |
Prolonged exposure of Stenotrophomonas maltophilia biofilms to trace levels of clofibric acid alters antimicrobial tolerance and virulence |
title_fullStr |
Prolonged exposure of Stenotrophomonas maltophilia biofilms to trace levels of clofibric acid alters antimicrobial tolerance and virulence |
title_full_unstemmed |
Prolonged exposure of Stenotrophomonas maltophilia biofilms to trace levels of clofibric acid alters antimicrobial tolerance and virulence |
title_sort |
Prolonged exposure of Stenotrophomonas maltophilia biofilms to trace levels of clofibric acid alters antimicrobial tolerance and virulence |
author |
Gomes, I.B. |
author_facet |
Gomes, I.B. Querido, M.M. Teixeira, J.P. Pereira, C.C. Simões, L.C. Simões, M. |
author_role |
author |
author2 |
Querido, M.M. Teixeira, J.P. Pereira, C.C. Simões, L.C. Simões, M. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Nacional de Saúde |
dc.contributor.author.fl_str_mv |
Gomes, I.B. Querido, M.M. Teixeira, J.P. Pereira, C.C. Simões, L.C. Simões, M. |
dc.subject.por.fl_str_mv |
Anti-Bacterial Agents Anti-Infective Agents Biofilms Ciprofloxacin Clofibric Acid Drinking Water Drug Tolerance HT29 Cells Humans Stenotrophomonas maltophilia Virulence Água e Solo |
topic |
Anti-Bacterial Agents Anti-Infective Agents Biofilms Ciprofloxacin Clofibric Acid Drinking Water Drug Tolerance HT29 Cells Humans Stenotrophomonas maltophilia Virulence Água e Solo |
description |
The presence of pharmaceuticals in water sources, including in drinking water (DW), is increasingly being recognized as an emerging and global concern for the environment and public health. Based on the principles of the "One Health" initiative, the present work aims to understand the effects of clofibric acid (CA), a lipid regulator, on the behavior of a selected bacterium isolated from drinking water (DW). Biofilms of the opportunistic pathogen Stenotrophomonas maltophilia were exposed to CA for 12 weeks at 170 and 17000 ng/L. The effects of CA were evaluated on planktonic S. maltophilia susceptibility to chlorine and antibiotics (amoxicillin, ciprofloxacin, erythromycin, kanamycin, levofloxacin, oxacillin, spectinomycin, tetracycline and trimethoprim-sulfamethoxazole), biofilm formation, motility, siderophores production and on the adhesion and internalization of the human colon adenocarcinoma cell line (HT-29). It was found that CA did not affect planktonic S. maltophilia tolerance to chlorine exposure. Additionally, no effects were observed on biofilm formation, motility and siderophores production. However, biofilms formed after CA exposure were more tolerant to chlorine disinfection and lower CFU reductions were obtained. Of additional concern was the effect of CA exposure on S. maltophilia increased tolerance to erythromycin. CA exposure also slightly reduced S. maltophilia ability to invade HT-29 cells. In conclusion, this work reinforces the importance of studying the effects of non-antibiotic contaminants on the behavior of environmental microorganisms, particularly their role as drivers affecting resistance evolution and selection. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-11 2019-11-01T00:00:00Z 2020-05-09T11:07:46Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.18/6626 |
url |
http://hdl.handle.net/10400.18/6626 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Chemosphere. 2019 Nov;235:327-335. doi: 10.1016/j.chemosphere.2019.06.184. Epub 2019 Jun 25 0045-6535 10.1016/j.chemosphere.2019.06.184 |
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info:eu-repo/semantics/embargoedAccess |
eu_rights_str_mv |
embargoedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier/ Pergamon |
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Elsevier/ Pergamon |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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