Glucose production, gluconeogenesis, and hepatic tricarboxylic acid cycle fluxes measured by nuclear magnetic resonance analysis of a single glucose derivative
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/3869 https://doi.org/10.1016/j.ab.2003.12.036 |
Resumo: | A triple-tracer method was developed to provide absolute fluxes contributing to endogenous glucose production and hepatic tricarboxylic acid (TCA) cycle fluxes in 24-h-fasted rats by 2H and 13C nuclear magnetic resonance (NMR) analysis of a single glucose derivative. A primed, intravenous [3,4-13C2]glucose infusion was used to measure endogenous glucose production; intraperitoneal 2H2O (to enrich total body water) was used to quantify sources of glucose (TCA cycle, glycerol, and glycogen), and intraperitoneal [U-13C3] propionate was used to quantify hepatic anaplerosis, pyruvate cycling, and TCA cycle flux. Plasma glucose was converted to monoacetone glucose (MAG), and a single 2H and 13C NMR spectrum of MAG provided the following metabolic data (all in units of [mu]mol/kg/min; n=6): endogenous glucose production (40.4 ± 2.9), gluconeogenesis from glycerol (11.5 ± 3.5), gluconeogenesis from the TCA cycle (67.3 ± 5.6), glycogenolysis (1.0 ± 0.8), pyruvate cycling (154.4 ± 43.4), PEPCK flux (221.7 ± 47.6), and TCA cycle flux (49.1 ± 16.8). In a separate group of rats, glucose production was not different in the absence of 2H2O and [U-13C]propionate, demonstrating that these tracers do not alter the measurement of glucose turnover. |
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Glucose production, gluconeogenesis, and hepatic tricarboxylic acid cycle fluxes measured by nuclear magnetic resonance analysis of a single glucose derivativeLiver metabolismGlucose turnoverGluconeogenesisStable isotope tracersCitric acid cycleA triple-tracer method was developed to provide absolute fluxes contributing to endogenous glucose production and hepatic tricarboxylic acid (TCA) cycle fluxes in 24-h-fasted rats by 2H and 13C nuclear magnetic resonance (NMR) analysis of a single glucose derivative. A primed, intravenous [3,4-13C2]glucose infusion was used to measure endogenous glucose production; intraperitoneal 2H2O (to enrich total body water) was used to quantify sources of glucose (TCA cycle, glycerol, and glycogen), and intraperitoneal [U-13C3] propionate was used to quantify hepatic anaplerosis, pyruvate cycling, and TCA cycle flux. Plasma glucose was converted to monoacetone glucose (MAG), and a single 2H and 13C NMR spectrum of MAG provided the following metabolic data (all in units of [mu]mol/kg/min; n=6): endogenous glucose production (40.4 ± 2.9), gluconeogenesis from glycerol (11.5 ± 3.5), gluconeogenesis from the TCA cycle (67.3 ± 5.6), glycogenolysis (1.0 ± 0.8), pyruvate cycling (154.4 ± 43.4), PEPCK flux (221.7 ± 47.6), and TCA cycle flux (49.1 ± 16.8). In a separate group of rats, glucose production was not different in the absence of 2H2O and [U-13C]propionate, demonstrating that these tracers do not alter the measurement of glucose turnover.http://www.sciencedirect.com/science/article/B6W9V-4BWYNW7-2/1/140b73c9df39bb7829a8519979c37a6e2004info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleaplication/PDFhttp://hdl.handle.net/10316/3869http://hdl.handle.net/10316/3869https://doi.org/10.1016/j.ab.2003.12.036engAnalytical Biochemistry. 327:2 (2004) 149-155Jin, Eunsook S.Jones, John G.Merritt, MatthewBurgess, Shawn C.Malloy, Craig R.Sherry, A. Deaninfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2021-10-26T13:39:35Zoai:estudogeral.uc.pt:10316/3869Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:55:42.658129Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Glucose production, gluconeogenesis, and hepatic tricarboxylic acid cycle fluxes measured by nuclear magnetic resonance analysis of a single glucose derivative |
title |
Glucose production, gluconeogenesis, and hepatic tricarboxylic acid cycle fluxes measured by nuclear magnetic resonance analysis of a single glucose derivative |
spellingShingle |
Glucose production, gluconeogenesis, and hepatic tricarboxylic acid cycle fluxes measured by nuclear magnetic resonance analysis of a single glucose derivative Jin, Eunsook S. Liver metabolism Glucose turnover Gluconeogenesis Stable isotope tracers Citric acid cycle |
title_short |
Glucose production, gluconeogenesis, and hepatic tricarboxylic acid cycle fluxes measured by nuclear magnetic resonance analysis of a single glucose derivative |
title_full |
Glucose production, gluconeogenesis, and hepatic tricarboxylic acid cycle fluxes measured by nuclear magnetic resonance analysis of a single glucose derivative |
title_fullStr |
Glucose production, gluconeogenesis, and hepatic tricarboxylic acid cycle fluxes measured by nuclear magnetic resonance analysis of a single glucose derivative |
title_full_unstemmed |
Glucose production, gluconeogenesis, and hepatic tricarboxylic acid cycle fluxes measured by nuclear magnetic resonance analysis of a single glucose derivative |
title_sort |
Glucose production, gluconeogenesis, and hepatic tricarboxylic acid cycle fluxes measured by nuclear magnetic resonance analysis of a single glucose derivative |
author |
Jin, Eunsook S. |
author_facet |
Jin, Eunsook S. Jones, John G. Merritt, Matthew Burgess, Shawn C. Malloy, Craig R. Sherry, A. Dean |
author_role |
author |
author2 |
Jones, John G. Merritt, Matthew Burgess, Shawn C. Malloy, Craig R. Sherry, A. Dean |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Jin, Eunsook S. Jones, John G. Merritt, Matthew Burgess, Shawn C. Malloy, Craig R. Sherry, A. Dean |
dc.subject.por.fl_str_mv |
Liver metabolism Glucose turnover Gluconeogenesis Stable isotope tracers Citric acid cycle |
topic |
Liver metabolism Glucose turnover Gluconeogenesis Stable isotope tracers Citric acid cycle |
description |
A triple-tracer method was developed to provide absolute fluxes contributing to endogenous glucose production and hepatic tricarboxylic acid (TCA) cycle fluxes in 24-h-fasted rats by 2H and 13C nuclear magnetic resonance (NMR) analysis of a single glucose derivative. A primed, intravenous [3,4-13C2]glucose infusion was used to measure endogenous glucose production; intraperitoneal 2H2O (to enrich total body water) was used to quantify sources of glucose (TCA cycle, glycerol, and glycogen), and intraperitoneal [U-13C3] propionate was used to quantify hepatic anaplerosis, pyruvate cycling, and TCA cycle flux. Plasma glucose was converted to monoacetone glucose (MAG), and a single 2H and 13C NMR spectrum of MAG provided the following metabolic data (all in units of [mu]mol/kg/min; n=6): endogenous glucose production (40.4 ± 2.9), gluconeogenesis from glycerol (11.5 ± 3.5), gluconeogenesis from the TCA cycle (67.3 ± 5.6), glycogenolysis (1.0 ± 0.8), pyruvate cycling (154.4 ± 43.4), PEPCK flux (221.7 ± 47.6), and TCA cycle flux (49.1 ± 16.8). In a separate group of rats, glucose production was not different in the absence of 2H2O and [U-13C]propionate, demonstrating that these tracers do not alter the measurement of glucose turnover. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/3869 http://hdl.handle.net/10316/3869 https://doi.org/10.1016/j.ab.2003.12.036 |
url |
http://hdl.handle.net/10316/3869 https://doi.org/10.1016/j.ab.2003.12.036 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Analytical Biochemistry. 327:2 (2004) 149-155 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
aplication/PDF |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133843696910336 |