Flexible nanofilms coated with aligned piezoelectric microfibers preserve the contractility of cardiomyocytes
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/92388 https://doi.org/10.1016/j.biomaterials.2017.05.048 |
Resumo: | The use of engineered cardiac tissue for high-throughput drug screening/toxicology assessment remains largely unexplored. Here we propose a scaffold that mimics aspects of cardiac extracellular matrix while preserving the contractility of cardiomyocytes. The scaffold is based on a poly(caprolactone) (PCL) nanofilm with magnetic properties (MNF, standing for magnetic nanofilm) coated with a layer of piezoelectric (PIEZO) microfibers of poly(vinylidene fluoride-trifluoroethylene) (MNF+PIEZO). The nanofilm creates a flexible support for cell contraction and the aligned PIEZO microfibers deposited on top of the nanofilm creates conditions for cell alignment and electrical stimulation of the seeded cells. Our results indicate that MNF+PIEZO scaffold promotes rat and human cardiac cell attachment and alignment, maintains the ratio of cell populations overtime, promotes cell-cell communication and metabolic maturation, and preserves cardiomyocyte (CM) contractility for at least 12 days. The engineered cardiac construct showed high toxicity against doxorubicin, a cardiotoxic molecule, and responded to compounds that modulate CM contraction such as epinephrine, propranolol and heptanol. |
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Flexible nanofilms coated with aligned piezoelectric microfibers preserve the contractility of cardiomyocytesCardiac tissue engineering; Cardiotoxicity; Electrospun fibers; Nanofilms; Piezoelectric materialsAnimalsAnti-Arrhythmia AgentsCell CommunicationCells, CulturedCoculture TechniquesDoxorubicinDrug Evaluation, PreclinicalElectric StimulationExtracellular MatrixHumansHydrocarbons, FluorinatedMagnetic PhenomenaMyocardial ContractionMyocytes, CardiacNanostructuresPolyestersRatsRats, WistarTime FactorsTissue EngineeringTissue ScaffoldsVasoconstrictor AgentsVinyl CompoundsThe use of engineered cardiac tissue for high-throughput drug screening/toxicology assessment remains largely unexplored. Here we propose a scaffold that mimics aspects of cardiac extracellular matrix while preserving the contractility of cardiomyocytes. The scaffold is based on a poly(caprolactone) (PCL) nanofilm with magnetic properties (MNF, standing for magnetic nanofilm) coated with a layer of piezoelectric (PIEZO) microfibers of poly(vinylidene fluoride-trifluoroethylene) (MNF+PIEZO). The nanofilm creates a flexible support for cell contraction and the aligned PIEZO microfibers deposited on top of the nanofilm creates conditions for cell alignment and electrical stimulation of the seeded cells. Our results indicate that MNF+PIEZO scaffold promotes rat and human cardiac cell attachment and alignment, maintains the ratio of cell populations overtime, promotes cell-cell communication and metabolic maturation, and preserves cardiomyocyte (CM) contractility for at least 12 days. The engineered cardiac construct showed high toxicity against doxorubicin, a cardiotoxic molecule, and responded to compounds that modulate CM contraction such as epinephrine, propranolol and heptanol.This work was supported by funds from FEDER through COMPETE program and Fundação para a Ciência e a Tecnologia (FCT) (EXPL/DTP-FTO/0570/2012, MITPTB/ ECE/0013/2013 to S. R. and L.F., and PTDC/SAU-ENB/113696/2009 to R.P.N.; SFRH/BD/51197/2010 and SFRH/BPD/79323/2011 to P.G. and S.R., respectively) as well as COMPETE program for the project “Stem cell based platforms for Regenerative and Therapeutic Medicine” (Centro-07-ST24-FEDER-002008). The authors also would like to acknowledge the help of Tommaso Mazzochi for the finite model simulations.Elsevier2017-09info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/92388http://hdl.handle.net/10316/92388https://doi.org/10.1016/j.biomaterials.2017.05.048eng01429612Gouveia, P JoséRosa, S.Ricotti, L.Abecasis, B.Almeida, H. V.Monteiro, L. R.Nunes, J.Carvalho, F. SofiaSerra, M.Luchkin, S.Kholkin, A. LeonidovitchAlves, P. MarquesOliveira, P. JorgeCarvalho, R.Menciassi, A.Neves, R. Pires dasFerreira, L. Silvainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-04-06T10:20:15Zoai:estudogeral.uc.pt:10316/92388Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:11:29.759922Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Flexible nanofilms coated with aligned piezoelectric microfibers preserve the contractility of cardiomyocytes |
title |
Flexible nanofilms coated with aligned piezoelectric microfibers preserve the contractility of cardiomyocytes |
spellingShingle |
Flexible nanofilms coated with aligned piezoelectric microfibers preserve the contractility of cardiomyocytes Gouveia, P José Cardiac tissue engineering; Cardiotoxicity; Electrospun fibers; Nanofilms; Piezoelectric materials Animals Anti-Arrhythmia Agents Cell Communication Cells, Cultured Coculture Techniques Doxorubicin Drug Evaluation, Preclinical Electric Stimulation Extracellular Matrix Humans Hydrocarbons, Fluorinated Magnetic Phenomena Myocardial Contraction Myocytes, Cardiac Nanostructures Polyesters Rats Rats, Wistar Time Factors Tissue Engineering Tissue Scaffolds Vasoconstrictor Agents Vinyl Compounds |
title_short |
Flexible nanofilms coated with aligned piezoelectric microfibers preserve the contractility of cardiomyocytes |
title_full |
Flexible nanofilms coated with aligned piezoelectric microfibers preserve the contractility of cardiomyocytes |
title_fullStr |
Flexible nanofilms coated with aligned piezoelectric microfibers preserve the contractility of cardiomyocytes |
title_full_unstemmed |
Flexible nanofilms coated with aligned piezoelectric microfibers preserve the contractility of cardiomyocytes |
title_sort |
Flexible nanofilms coated with aligned piezoelectric microfibers preserve the contractility of cardiomyocytes |
author |
Gouveia, P José |
author_facet |
Gouveia, P José Rosa, S. Ricotti, L. Abecasis, B. Almeida, H. V. Monteiro, L. R. Nunes, J. Carvalho, F. Sofia Serra, M. Luchkin, S. Kholkin, A. Leonidovitch Alves, P. Marques Oliveira, P. Jorge Carvalho, R. Menciassi, A. Neves, R. Pires das Ferreira, L. Silva |
author_role |
author |
author2 |
Rosa, S. Ricotti, L. Abecasis, B. Almeida, H. V. Monteiro, L. R. Nunes, J. Carvalho, F. Sofia Serra, M. Luchkin, S. Kholkin, A. Leonidovitch Alves, P. Marques Oliveira, P. Jorge Carvalho, R. Menciassi, A. Neves, R. Pires das Ferreira, L. Silva |
author2_role |
author author author author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Gouveia, P José Rosa, S. Ricotti, L. Abecasis, B. Almeida, H. V. Monteiro, L. R. Nunes, J. Carvalho, F. Sofia Serra, M. Luchkin, S. Kholkin, A. Leonidovitch Alves, P. Marques Oliveira, P. Jorge Carvalho, R. Menciassi, A. Neves, R. Pires das Ferreira, L. Silva |
dc.subject.por.fl_str_mv |
Cardiac tissue engineering; Cardiotoxicity; Electrospun fibers; Nanofilms; Piezoelectric materials Animals Anti-Arrhythmia Agents Cell Communication Cells, Cultured Coculture Techniques Doxorubicin Drug Evaluation, Preclinical Electric Stimulation Extracellular Matrix Humans Hydrocarbons, Fluorinated Magnetic Phenomena Myocardial Contraction Myocytes, Cardiac Nanostructures Polyesters Rats Rats, Wistar Time Factors Tissue Engineering Tissue Scaffolds Vasoconstrictor Agents Vinyl Compounds |
topic |
Cardiac tissue engineering; Cardiotoxicity; Electrospun fibers; Nanofilms; Piezoelectric materials Animals Anti-Arrhythmia Agents Cell Communication Cells, Cultured Coculture Techniques Doxorubicin Drug Evaluation, Preclinical Electric Stimulation Extracellular Matrix Humans Hydrocarbons, Fluorinated Magnetic Phenomena Myocardial Contraction Myocytes, Cardiac Nanostructures Polyesters Rats Rats, Wistar Time Factors Tissue Engineering Tissue Scaffolds Vasoconstrictor Agents Vinyl Compounds |
description |
The use of engineered cardiac tissue for high-throughput drug screening/toxicology assessment remains largely unexplored. Here we propose a scaffold that mimics aspects of cardiac extracellular matrix while preserving the contractility of cardiomyocytes. The scaffold is based on a poly(caprolactone) (PCL) nanofilm with magnetic properties (MNF, standing for magnetic nanofilm) coated with a layer of piezoelectric (PIEZO) microfibers of poly(vinylidene fluoride-trifluoroethylene) (MNF+PIEZO). The nanofilm creates a flexible support for cell contraction and the aligned PIEZO microfibers deposited on top of the nanofilm creates conditions for cell alignment and electrical stimulation of the seeded cells. Our results indicate that MNF+PIEZO scaffold promotes rat and human cardiac cell attachment and alignment, maintains the ratio of cell populations overtime, promotes cell-cell communication and metabolic maturation, and preserves cardiomyocyte (CM) contractility for at least 12 days. The engineered cardiac construct showed high toxicity against doxorubicin, a cardiotoxic molecule, and responded to compounds that modulate CM contraction such as epinephrine, propranolol and heptanol. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-09 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/92388 http://hdl.handle.net/10316/92388 https://doi.org/10.1016/j.biomaterials.2017.05.048 |
url |
http://hdl.handle.net/10316/92388 https://doi.org/10.1016/j.biomaterials.2017.05.048 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
01429612 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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